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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 08-SEP-2024

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae BM6001
  • locus tag: ODS73_RS02700 [old locus tag: ODS73_02700 ]
  • pan locus tag?: PNEUPAN001319000
  • symbol: ODS73_RS02700
  • pan gene symbol?: rrgB
  • synonym:
  • product: isopeptide-forming domain-containing fimbrial protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: ODS73_RS02700 [old locus tag: ODS73_02700 ]
  • symbol: ODS73_RS02700
  • product: isopeptide-forming domain-containing fimbrial protein
  • replicon: chromosome
  • strand: +
  • coordinates: 499428..501392
  • length: 1965
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: NZ_CP107038 (499428..501392) NCBI
  • BioCyc:
  • MicrobesOnline:
  • PneumoBrowse for strain D39V:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAAATCAATCAACAAATTTTTAACAATGCTTGCTGCCTTATTACTGACAGCGAGTAGC
    CTGTTCTCAGCTGCAACAGTTTTTGCGGCGGAACAAAAAACTAAGACACTTACAGTTCAT
    AAATTATTGATGACAGATCAAGAGCTTGACGCTTGGAATTCTGATGCGATTACTACTGCA
    GGTTATGACGGTTCGCAAAATTTTGAACAGTTCAAACAACTTCAAGGTGTTCCACAAGGA
    GTAACCGAAATCTCTGGTGTTGCATTCGAGTTACAGAGTTATACGGGTCCTCAAGGAAAA
    GAACAAGAAAATTTAACGAATGATGCGGTTTGGACTGCGGTTAATAAAGGTGTGACGACT
    GAAACGGGTGTTAAATTTGATACTGAAGTTTTACAAGGGACATATCGTCTTGTCGAAGTA
    CGTAAAGAATCGACTTATGTCGGTCCAAATGGTAAAGTTTTAACAGGTATGAAAGCTGTT
    CCTGCTTTAATTACTCTGCCGCTTGTAAACCAAAATGGTGTTGTAGAAAATGCACATGTC
    TATCCAAAGAATTCTGAAGACAAACCTACAGCAACGAAAACATTTGATACTGCAGCAGGT
    TTCGTAGATCCAGGTGAAAAAGGTTTAGCAATTGGCACTAAGGTACCGTATATTGTTACA
    ACAACTATTCCGAAAAACTCAACTCTTGCAACAGCTTTCTGGTCAGATGAAATGACAGAA
    GGTCTAGATTATAATGGTGATGTAGTTGTTAATTATAATGGTCAACCGCTTGATAATTCT
    CATTACACATTAGAAGCAGGTCATAATGGCTTTATCTTGAAGTTAAATGAAAAAGGTCTG
    GAAGCAATCAACGGTAAAGATGCAGAAGCAACAATTACGTTGAAGTATACTGCAACTTTA
    AATGCTCTTGCTGTTGCTGATGTGCCAGAAGCGAATGATGTAACATTCCATTATGGAAAC
    AACCCAGGTCATGGTAACACTCCAAAACCAAACAAACCTAAAAACGGTGAACTTACAATT
    ACTAAAACATGGGCTGATGCTAAAGATGCTCCTATAGCAGGTGTAGAAGTAACTTTTGAT
    TTGGTAAATGCTCAGACAGGTGAGGTCGTTAAAGTACCTGGACATGAAACAGGTATTGTA
    TTGAATCAAACAAATAATTGGACATTTACTGCTACAGGTCTTGATAATAATACAGAATAT
    AAATTTGTTGAACGGACAATTAAGGGATATTCTGCAGATTACCAAACAATTACTGAAACA
    GGAAAAATTGCTGTTAAAAACTGGAAAGATGAAAATCCAGAACCAATAAATCCTGAAGAG
    CCACGTGTAAAAACATATGGTAAAAAATTCGTTAAGGTTGACCAAAAAGACGAACGCTTA
    AAAGAAGCACAATTCGTTGTGAAGAATGAGCAAGGGAAATATCTTGCACTCAAATCTGCA
    GCACAACAAGCTGTAAATGAGAAAGCTGCCGCAGAAGCGAAACAAGCGCTAGATGCAGCG
    ATAGCAGCCTATACAAATGCTGCAGATAAAAATGCAGCACAAGCTGTAGTAGATGCTGCG
    CAAAAAACATATAATGACAATTACAGAGCAGCTAGATTTGGCTATGTAGAAGTAGAGAGA
    AAAGAAGATGCGTTAGTTCTTACTTCTAACACTGATGGTCAATTCCAAATTTCAGGTCTT
    GCTGCTGGAAGCTACACGTTGGAAGAAACAAAAGCTCCAGAAGGCTTTGCAAAACTTGGA
    GATGTGAAGTTTGAGGTTGGAGCAGGTTCTTGGAATCAAGGTGATTTCAATTATTTAAAA
    GATGTTCAGAAGAACGACGCTACAAAAGTAGTCAACAAAAAAATCACGATCCCTCAAACG
    GGTGGTATTGGTACAATTATCTTTGCTGTAGCGGGGGCTGTGATTATGGGTATTGCAGTG
    TACGCATATGTTAAAAACAACAAAGATGAGGATCAACTTGCTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1965

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: ODS73_RS02700 [old locus tag: ODS73_02700 ]
  • symbol: ODS73_RS02700
  • description: isopeptide-forming domain-containing fimbrial protein
  • length: 654
  • theoretical pI: 4.91576
  • theoretical MW: 70738.7
  • GRAVY: -0.392049

⊟Function[edit | edit source]

  • TIGRFAM:
    fimbrial isopeptide formation D2 domain (TIGR04226; HMM-score: 79.8)
    and 1 more
    Cell structure Cell envelope Other LPXTG cell wall anchor domain (TIGR01167; HMM-score: 24.5)
  • TheSEED: data available for Hungary19A-6, TIGR4
  • PFAM:
    Transthyretin (CL0287) GramPos_pilinD3; Gram-positive pilin backbone subunit 3, Cna-B-like domain (PF16570; HMM-score: 109.6)
    and 7 more
    Adhesin (CL0204) GramPos_pilinBB; Gram-positive pilin backbone subunit 2, Cna-B-like domain (PF16569; HMM-score: 75.2)
    Transthyretin (CL0287) GramPos_pilinD1; Gram-positive pilin subunit D1, N-terminal domain (PF16555; HMM-score: 65.2)
    SpaA; Prealbumin-like fold domain (PF17802; HMM-score: 41.6)
    no clan defined Gram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 30.5)
    Transthyretin (CL0287) Cna_B; Cna protein B-type domain (PF05738; HMM-score: 26.1)
    CarboxypepD_reg; Carboxypeptidase regulatory-like domain (PF13620; HMM-score: 18.5)
    Pec_lyase-like (CL0268) AIDA; Adhesin of bacterial autotransporter system, probable stalk (PF16168; HMM-score: 15.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.32
    • Cytoplasmic Membrane Score: 9.55
    • Cellwall Score: 0.12
    • Extracellular Score: 0.01
    • Internal Helices: 2
  • DeepLocPro: Cell wall & surface
    • Cytoplasmic Score: 0.0001
    • Cytoplasmic Membrane Score: 0.1063
    • Cell wall & surface Score: 0.8765
    • Extracellular Score: 0.0172
  • SignalP: Signal peptide SP(Sec/SPI) length 29 aa
    • SP(Sec/SPI): 0.994898
    • TAT(Tat/SPI): 0.002808
    • LIPO(Sec/SPII): 0.001715
    • Cleavage Site: CS pos: 29-30. VFA-AE. Pr: 0.9816
  • predicted transmembrane helices (TMHMM): 2

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_061756053 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKSINKFLTMLAALLLTASSLFSAATVFAAEQKTKTLTVHKLLMTDQELDAWNSDAITTAGYDGSQNFEQFKQLQGVPQGVTEISGVAFELQSYTGPQGKEQENLTNDAVWTAVNKGVTTETGVKFDTEVLQGTYRLVEVRKESTYVGPNGKVLTGMKAVPALITLPLVNQNGVVENAHVYPKNSEDKPTATKTFDTAAGFVDPGEKGLAIGTKVPYIVTTTIPKNSTLATAFWSDEMTEGLDYNGDVVVNYNGQPLDNSHYTLEAGHNGFILKLNEKGLEAINGKDAEATITLKYTATLNALAVADVPEANDVTFHYGNNPGHGNTPKPNKPKNGELTITKTWADAKDAPIAGVEVTFDLVNAQTGEVVKVPGHETGIVLNQTNNWTFTATGLDNNTEYKFVERTIKGYSADYQTITETGKIAVKNWKDENPEPINPEEPRVKTYGKKFVKVDQKDERLKEAQFVVKNEQGKYLALKSAAQQAVNEKAAAEAKQALDAAIAAYTNAADKNAAQAVVDAAQKTYNDNYRAARFGYVEVERKEDALVLTSNTDGQFQISGLAAGSYTLEETKAPEGFAKLGDVKFEVGAGSWNQGDFNYLKDVQKNDATKVVNKKITIPQTGGIGTIIFAVAGAVIMGIAVYAYVKNNKDEDQLA

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription[edit | edit source]

  • transcription start site:

⊟Expression data[edit | edit source]

  • PneumoExpress for strain D39V:

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Julianna LeMieux, David L Hava, Alan Basset, Andrew Camilli
RrgA and RrgB are components of a multisubunit pilus encoded by the Streptococcus pneumoniae rlrA pathogenicity islet.
Infect Immun: 2006, 74(4);2453-6
[PubMed:16552078] [WorldCat.org] [DOI] (P p)Tanja D Becke, Stefan Ness, Benedikt K Kaufmann, Bastian Hartmann, Arndt F Schilling, Stefanie Sudhop, Markus Hilleringmann, Hauke Clausen-Schaumann
Pilus-1 Backbone Protein RrgB of Streptococcus pneumoniae Binds Collagen I in a Force-Dependent Way.
ACS Nano: 2019, 13(6);7155-7165
[PubMed:31184856] [WorldCat.org] [DOI] (I p)

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