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serotype 4
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serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
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670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
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HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
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serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 31-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae 70585
  • locus tag: SP70585_0811 [new locus tag: SP70585_RS03995 ]
  • pan locus tag?: PNEUPAN001707000
  • symbol: SP70585_0811
  • pan gene symbol?: rlmN
  • synonym:
  • product: radical SAM enzyme, Cfr family

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SP70585_0811 [new locus tag: SP70585_RS03995 ]
  • symbol: SP70585_0811
  • product: radical SAM enzyme, Cfr family
  • replicon: chromosome
  • strand: +
  • coordinates: 743921..745006
  • length: 1086
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAAACCCTCAATTCATAGTTTGGCACATCAAACCATGCAGGAATGGGTATTGGAGCAG
    GGAGAAAAGAAATTCCGTGCAGATCAAATCTGGGAATGGCTCTACCGTAAACGTGTGCAG
    TCATTTGAAGAAATGACCAACCTTTCCAAGGATTTGATTGCTAAGCTCAATGACCAGTTT
    GTGGTCAATCCCTTGAAACAACGTATTGTGCAAGAGTCTGCTGATGGTACAGTCAAATAT
    CTCTTTGAATTGCCTGACGGTATGTTAATTGAGACTGTACTCATGTGTCAACACTATGGT
    TTATCTGTCTGTGTGACCACTCAGGTCGGCTGTAATATCGGTTGTACCTTCTGTGCCTCT
    GGTTTGATTAAGAAGCAGCGTGACCTCAATAACGGTGAAATCGTAGCGCAAATCATGCTG
    GTTCAGAAATACTTTGATGAGCGTGGTCAGGATGAACGCGTCAGCCATATCGTTGTCATG
    GGAATCGGTGAGCCCTTTGATAATTACAACAATGTCTTGAATTTCTTTCGTACTATCAAT
    GATGATAAAGGAATGGCTATCGGTGCTCGTCACATCACGGTTTCAACCTCAGGTTTGGCT
    CATAAAATTCGTGACTTTGCTGATGAAGGGGTTCAGGTCAATCTTGCCGTGTCTCTTCAC
    GCACCTAACAATGAACTTCGTTCAAGTATCATGAAGATTAACCGTGCCTTTCCGATTGAA
    AAACTCTTTGCTGCTATTGAATACTACATTGAGACAACAAACCGTGGTGTAACCTTTGAA
    TATATCATGCTTAATGAAGTCAACGACGGTGTAGAACAAGCCTTGGAATTGACTGAATTG
    CTCAAGAACATCAAGAAATTGTCTTATGTAAACTTGATTCCTTATAACCCAGTTAGTGAG
    CATGACCAATATAGCCGTAGTCCCAAAGAGCGCGTGTTGGCTTTCTATGATACGCTTAAG
    AAAAAAGGGGTCAACTGTGTGGTTCGTCAAGAGCATGGTACAGATATTGACGCAGCTTGT
    GGACAATTGCGCTCTAACACAATGAAACGTGACCGCCAGAAGGCAGTCTCAGCGGTAAAT
    CCTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1086

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SP70585_0811 [new locus tag: SP70585_RS03995 ]
  • symbol: SP70585_0811
  • description: radical SAM enzyme, Cfr family
  • length: 361
  • theoretical pI: 6.9906
  • theoretical MW: 41157.9
  • GRAVY: -0.325208

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification 23S rRNA (adenine(2503)-C(2))-methyltransferase (TIGR00048; EC 2.1.1.192; HMM-score: 440.5)
    and 11 more
    Genetic information processing Protein synthesis tRNA and rRNA base modification 23S rRNA (adenine(2503)-C(8))-methyltransferase Cfr (TIGR04432; EC 2.1.1.224; HMM-score: 245.9)
    Genetic information processing Protein fate Protein modification and repair pyruvate formate-lyase 1-activating enzyme (TIGR02493; EC 1.97.1.4; HMM-score: 32.1)
    Metabolism Energy metabolism Anaerobic pyruvate formate-lyase 1-activating enzyme (TIGR02493; EC 1.97.1.4; HMM-score: 32.1)
    tungsten cofactor oxidoreducase radical SAM maturase (TIGR04317; HMM-score: 31.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Molybdopterin molybdenum cofactor biosynthesis protein A (TIGR02666; HMM-score: 28.8)
    radical SAM enzyme, TIGR04100 family (TIGR04100; HMM-score: 18.5)
    His-Xaa-Ser system radical SAM maturase HxsC (TIGR03977; HMM-score: 18.2)
    Genetic information processing Protein fate Protein modification and repair quinohemoprotein amine dehydrogenase maturation protein (TIGR03906; HMM-score: 16.4)
    Genetic information processing Protein fate Protein modification and repair anaerobic ribonucleoside-triphosphate reductase activating protein (TIGR02495; EC 1.97.-.-; HMM-score: 13)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides 2'-Deoxyribonucleotide metabolism anaerobic ribonucleoside-triphosphate reductase activating protein (TIGR02495; EC 1.97.-.-; HMM-score: 13)
    Genetic information processing Protein synthesis tRNA and rRNA base modification MiaB-like tRNA modifying enzyme (TIGR01579; HMM-score: 12.9)
  • TheSEED  :
    • Ribosomal RNA large subunit methyltransferase N (EC 2.1.1.-)
    Conserved gene cluster associated with Met-tRNA formyltransferase  Ribosomal RNA large subunit methyltransferase N (EC 2.1.1.-)
  • PFAM:
    TIM_barrel (CL0036) Radical_SAM; Radical SAM superfamily (PF04055; HMM-score: 57.7)
    and 6 more
    4Fe-4S (CL0344) Fer4_14; 4Fe-4S single cluster domain (PF13394; HMM-score: 34.5)
    Fer4_12; 4Fe-4S single cluster domain (PF13353; HMM-score: 20.5)
    TIM_barrel (CL0036) Radical_SAM_2; Radical SAM-like domain (PF19238; HMM-score: 14.7)
    HTH (CL0123) TFIIE_beta; TFIIE beta subunit core domain (PF02186; HMM-score: 13.2)
    no clan defined P63C; P63C domain (PF10546; HMM-score: 13.1)
    zf-C2HC5; Putative zinc finger motif, C2HC5-type (PF06221; HMM-score: 12.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9784
    • Cytoplasmic Membrane Score: 0.0098
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.0117
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003248
    • TAT(Tat/SPI): 0.000218
    • LIPO(Sec/SPII): 0.00044
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKPSIHSLAHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEEMTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMCQHYGLSVCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFDERGQDERVSHIVVMGIGEPFDNYNNVLNFFRTINDDKGMAIGARHITVSTSGLAHKIRDFADEGVQVNLAVSLHAPNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRGVTFEYIMLNEVNDGVEQALELTELLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGVNCVVRQEHGTDIDAACGQLRSNTMKRDRQKAVSAVNP

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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