PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BM6001

serotype 19F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7465

serotype 1

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 15-DEC-2024

⊟Summary

• organism: Streptococcus pneumoniae 70585
• locus tag: SP70585_RS01955 [old locus tag: SP70585_0397 ]
• pan locus tag^?: PNEUPAN001089000
• symbol: rsmH
• pan gene symbol^?: rsmH
• synonym: mraW
• product: 16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SP70585_RS01955 [old locus tag: SP70585_0397 ]
• symbol: rsmH
• product: 16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH
• replicon: chromosome
• strand: +
• coordinates: 349727..350677
• length: 951
• essential: unknown other strains

⊟Accession numbers

• Location: NC_012468 (349727..350677) NCBI
• BioCyc: SP70585_RS01955 BioCyc
• MicrobesOnline: see SP70585_0397
• PneumoBrowse for strain D39V: SPV_0304 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  
  ATGACAAAAGAATTTCATCATGTAACGGTCTTACTCCACGAAACGATTGATATGCTTGAC
  GTAAAACCTGACGGTATCTACGTTGATGCGACTTTGGGTGGAGCAGGCCATAGCGAGTAT
  TTATTAAGTAAATTAAGTGAAAAAGGCCATCTCTATGCCTTTGACCAGGATCAGAATGCC
  ATTGACAATGCGCAAAAACGCTTGGCACCTTACATTGAGAAGGGAATGGTGACCTTTATC
  AAGGACAACTTCCGTCATTTACAGGCACGTTTGCGCGAAGCTGGTGTTCAGGAAATTGAT
  GGAATTTGTTATGACTTGGGAGTGTCTAGTCCTCAATTGGACCAGCGTGAGCGTGGTTTT
  TCTTATAAAAAGGATGCGCCACTGGACATGCGGATGAATCAGGATGCTAGTCTGACAGCC
  TATGAAGTGGTGAACAATTATGACTATCATGACTTGGTTCGTATTTTCTTCAAGTATGGA
  GAGGATAAATTCTCTAAACAGATTGCGCGTAAGATTGAGCAAGCGCGTGAAGTGAAGCCG
  ATTGAGACAACGACTGAGTTAGCAGAGATTATCAAGTTGGTCAAACCTGCCAAGGAACTC
  AAGAAGAAGGGGCATCCTGCTAAGCAGATTTTCCAGGCTATTCGAATTGAAGTCAATGAT
  GAACTGGGAGCGGCAGATGAGTCCATCCAGCAGGCTATGGATATGTTGGCTCTGGATGGT
  AGAATTTCAGTGATTACCTTTCATTCCTTAGAAGACCGCTTGACCAAGCAATTGTTCAAG
  GAAGCTTCAACAGTTGAAGTTCCAAAAGGCTTGCCTTTCATCCCAGATGATCTCAAGCCC
  AAGATGGAATTGGTGTCCCGTAAGCCAATCTTGCCAAGTGCGGAAGAGTTAGAAGCCAAT
  AACCGCTCGCACTCAGCCAAGTTGCGCGTGGCCAGAAAAATTCACAAGTAA
  60
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  840
  900
  951
  

⊟Protein

⊟General

• locus tag: SP70585_RS01955 [old locus tag: SP70585_0397 ]
• symbol: RsmH
• description: 16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH
• length: 316
• theoretical pI: 6.92678
• theoretical MW: 36000
• GRAVY: -0.500633

⊟Function

• reaction:
  EC 2.1.1.199?  ExPASy
  16S rRNA (cytosine¹⁴⁰²-N⁴)-methyltransferase S-adenosyl-L-methionine + cytosine¹⁴⁰² in 16S rRNA = S-adenosyl-L-homocysteine + N⁴-methylcytosine¹⁴⁰² in 16S rRNA
• TIGRFAM:
  Genetic information processing Protein synthesis tRNA and rRNA base modification 16S rRNA (cytosine(1402)-N(4))-methyltransferase (TIGR00006; EC 2.1.1.199; HMM-score: 394.3)
• TheSEED: see SP70585_0397
• PFAM:
  NADP_Rossmann (CL0063) Methyltransf_5; MraW methylase family (PF01795; HMM-score: 451.4)
  and 7 more
  rRNA_methylase; Putative rRNA methylase (PF06962; HMM-score: 22.3)
  GCD14; tRNA methyltransferase complex GCD14 subunit (PF08704; HMM-score: 18.5)
  Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 17.7)
  Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 15.5)
  FtsJ; FtsJ-like methyltransferase (PF01728; HMM-score: 15.3)
  HTH (CL0123) PRC2_HTH_1; Polycomb repressive complex 2 tri-helical domain (PF18118; HMM-score: 13.9)
  no clan defined FlhC; Flagellar transcriptional activator (FlhC) (PF05280; HMM-score: 12)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9984
  • Cytoplasmic Membrane Score: 0.0001
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.0016
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.006045
  • TAT(Tat/SPI): 0.000307
  • LIPO(Sec/SPII): 0.000392
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: WP_000159408 NCBI
• UniProt: see SP70585_0397

⊟Protein sequence

• MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSKLSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGICYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNNYDYHDLVRIFFKYGEDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEVNDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPDDLKPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: rsmH > ftsL > pbp2X > mraY

⊟Regulation

• regulator:

⊟Transcription

• transcription start site:

⊟Expression data

• PneumoExpress for strain D39V: SPV_0304 PneumoExpress

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications