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NCBI: 17-JAN-2018

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae AP200
  • locus tag: SPAP_0913 [new locus tag: SPAP_RS04535 ]
  • pan locus tag?: PNEUPAN001849000
  • symbol: SPAP_0913
  • pan gene symbol?: hsdM_2
  • synonym:
  • product: Type I restriction-modification system methyltransferase subunit

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPAP_0913 [new locus tag: SPAP_RS04535 ]
  • symbol: SPAP_0913
  • product: Type I restriction-modification system methyltransferase subunit
  • replicon: chromosome
  • strand: +
  • coordinates: 871308..872099
  • length: 792
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGTTTCATGGAAATGATACGGATACGACTATGTTGAGACTTGGGGCGATGAACATGATG
    CTACATGGAGTAGAAAATCCACAAATCAGTTACCTTGACTCGCTGTCTCAAGATAATGAA
    GAAGCCGATAAATATACTTTGGTTTTAGCAAATCCTCCTTTTAAGGGCTCACTTGACTAC
    AATTCAACCTCTAATGACCTTCTTGCAACCGTAAAAACCAAAAAAACAGAATTACTCTTT
    CTTTCTCTTTTCTTGCGAACTTTAAAACCAGGTGGACGAGCAGCAGTTATCGTACCTGAT
    GGTGTCCTTTTTGGTTCGTCTAAAGCTCATAAAGGAATTCGTCAGGAAATTGTAGAGAAT
    CATAAGCTTGATGCTGTAATCTCAATGCCTAGTGGTGTGTTCAAGCCTTATGCTGGAGTT
    TCAACTGCCATTCTCATCTTTACAAAAACTGGTAATGGTGGTACTGACAAAGTCTGGTTT
    TACGATATGAAAGCGGATGGTTTAAGTTTGGATGATAAGCGACAACCGATTAGCGACAAT
    GATATTCCAGATATTATCGAACGCTTTCATCATCTTGAAAAAGAAGCAGAACGTCAGAGA
    ACGGATCAATCTTTCTTTGTTCCAGTTGCTGAGATAAAGGAAAATGATTATGATTTGTCT
    ATCAATAAATATAAAGAGATTGAGTATGAAAAAGTTGAGTATGAACCAACAGAAGTCATA
    TTAAAGAAAATCAATGATTTAGAAAAAGAAATTCAAGCTGGCTTGGCTGAATTGGAAAAA
    TTACTCAAGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    792

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPAP_0913 [new locus tag: SPAP_RS04535 ]
  • symbol: SPAP_0913
  • description: Type I restriction-modification system methyltransferase subunit
  • length: 263
  • theoretical pI: 4.88288
  • theoretical MW: 29645.5
  • GRAVY: -0.412167

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism Restriction/modification type I restriction-modification system, M subunit (TIGR00497; EC 2.1.1.72; HMM-score: 70.3)
  • TheSEED  :
    • Type I restriction-modification system, DNA-methyltransferase subunit M (EC 2.1.1.72)
    DNA Metabolism DNA Metabolism - no subcategory Restriction-Modification System  Type I restriction-modification system, DNA-methyltransferase subunit M (EC 2.1.1.72)
    and 1 more
    DNA Metabolism DNA Metabolism - no subcategory Type I Restriction-Modification  Type I restriction-modification system, DNA-methyltransferase subunit M (EC 2.1.1.72)
  • PFAM:
    NADP_Rossmann (CL0063) N6_Mtase; N-6 DNA Methylase (PF02384; HMM-score: 166.7)
    and 1 more
    MTS; Methyltransferase small domain (PF05175; HMM-score: 14.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.8703
    • Cytoplasmic Membrane Score: 0.0549
    • Cell wall & surface Score: 0.0035
    • Extracellular Score: 0.0712
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.016288
    • TAT(Tat/SPI): 0.001748
    • LIPO(Sec/SPII): 0.002425
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: ADM84502 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEADKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPDGVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKVWFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKENDYDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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