PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 07-FEB-2021

⊟Summary

• organism: Streptococcus pneumoniae AP200
• locus tag: SPAP_RS03395 [old locus tag: SPAP_0684 ]
• pan locus tag^?: PNEUPAN001651000
• symbol: SPAP_RS03395
• pan gene symbol^?: glnH3
• synonym:
• product: cysteine ABC transporter substrate-binding protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SPAP_RS03395 [old locus tag: SPAP_0684 ]
• symbol: SPAP_RS03395
• product: cysteine ABC transporter substrate-binding protein
• replicon: chromosome
• strand: -
• coordinates: 657285..658151
• length: 867
• essential: unknown

⊟Accession numbers

• Location: NC_014494 (657285..658151) NCBI
• BioCyc: SPAP_RS03395 BioCyc
• MicrobesOnline:
• PneumoBrowse for strain D39V: SPV_0615 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  
  ATGAAACTATTCAAACCACTCTTAACTGTTTTAGTACTTGCCTTTGCCCTTATCTTTATC
  ACTGCTTGTAGCTCAGGTGGAAACGCTGGTTCATCCTCTGGAAAAACAACTGCCAAAGCT
  CGCACTATCGATGAAATCAAAAAAAGCGGTGAACTGCGAATCGCCGTGTTTGGAGATAAA
  AAACTGTTTGGCTACGTTGACAATGATGGTTCTTACCAAGGCTACGATATTGAACTAGGG
  AACCAACTAGCTCAAAACCTTGGTGTCAAGGTTAAATACATTTCAGTCGATGCTGCCAAC
  CGTGCGGAATACTTGATTTCAAACAAGGTAGATATTATTCTTGCTAACTTTACAGTAACT
  GACGAACGTAAGAAACAAGTTGATTTTGCCCTTCCATATATGAAAGTTTCTCTGGGTGTC
  GTATCACCTAAGACTGGTCTCATTACAGACGTCAAACAACTTGAAGGTAAAACCTTAATT
  GTCACAAAAGGAACGACTGCTGAGACTTATTTTGAAAAGAATCATCCAGAAATCAAACTC
  CAAAAATACGACCAATACAGTGACTCTTACCAAGCTCTTCTTGACGGACGTGGAGATGCC
  TTTTCAACTGATAATACGGAAGTTCTAGCTTGGGCGCTTGAAAATAAAGGATTTGAAGTA
  GGAATTACTTCCCTCGGTGATCCCGATACCATTGCGGCAGCAGTTCAAAAAGGCAACCAA
  GAATTGCTAGACTTCATCAATAAAGATATTGAAAAATTAGGCAAGGAAAACTTCTTCCAC
  AAGGCCTATGAAAAAACACTTCACCCAACCTACGGTGACACTGCTAAAGCAGATGACCTG
  GTTGTTGAAGGTGGAAAAGTTGATTAG
  60
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  840
  867
  

⊟Protein

⊟General

• locus tag: SPAP_RS03395 [old locus tag: SPAP_0684 ]
• symbol: SPAP_RS03395
• description: cysteine ABC transporter substrate-binding protein
• length: 288
• theoretical pI: 5.24366
• theoretical MW: 31516.5
• GRAVY: -0.248611

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Amino acids, peptides and amines lysine-arginine-ornithine-binding periplasmic protein (TIGR01096; HMM-score: 125.7)
  and 6 more
  extracellular substrate-binding orphan protein, GRRM family (TIGR04262; HMM-score: 54.8)
  Metabolism Transport and binding proteins Amino acids, peptides and amines ectoine/hydroxyectoine ABC transporter solute-binding protein EhuB (TIGR02995; HMM-score: 43.2)
  glycosyl amidation-associated protein WbuZ (TIGR03572; HMM-score: 12.4)
  Metabolism Transport and binding proteins Other ABC transporter, substrate-binding protein, aliphatic sulfonates family (TIGR01728; HMM-score: 11.9)
  Cellular processes Cellular processes Pathogenesis type III secretion apparatus protein, YscR/HrcR family (TIGR01102; HMM-score: 11.5)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking type III secretion apparatus protein, YscR/HrcR family (TIGR01102; HMM-score: 11.5)
• TheSEED: see SPAP_0684
• PFAM:
  PBP (CL0177) SBP_bac_3; Bacterial extracellular solute-binding proteins, family 3 (PF00497; HMM-score: 161.7)
  and 4 more
  Lig_chan-Glu_bd; Ligated ion channel L-glutamate- and glycine-binding site (PF10613; HMM-score: 36.5)
  Hybrid (CL0105) Biotin_lipoyl; Biotin-requiring enzyme (PF00364; HMM-score: 14.6)
  PBP (CL0177) OpuAC; Substrate binding domain of ABC-type glycine betaine transport system (PF04069; HMM-score: 14.1)
  no clan defined ODV-E18; Occlusion-derived virus envelope protein ODV-E18 (PF10717; HMM-score: 13.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helix: 1
• SignalP: Signal peptide LIPO(Sec/SPII) length 22 aa
  • SP(Sec/SPI): 0.002345
  • TAT(Tat/SPI): 0.000244
  • LIPO(Sec/SPII): 0.997227
  • Cleavage Site: CS pos: 22-23. ITA-CS. Pr: 0.9975
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI:
• RefSeq: WP_000765268 NCBI
• UniProt:

⊟Protein sequence

• MKLFKPLLTVLVLAFALIFITACSSGGNAGSSSGKTTAKARTIDEIKKSGELRIAVFGDKKLFGYVDNDGSYQGYDIELGNQLAQNLGVKVKYISVDAANRAEYLISNKVDIILANFTVTDERKKQVDFALPYMKVSLGVVSPKTGLITDVKQLEGKTLIVTKGTTAETYFEKNHPEIKLQKYDQYSDSYQALLDGRGDAFSTDNTEVLAWALENKGFEVGITSLGDPDTIAAAVQKGNQELLDFINKDIEKLGKENFFHKAYEKTLHPTYGDTAKADDLVVEGGKVD

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Expression data

• PneumoExpress for strain D39V: SPV_0615 PneumoExpress

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications