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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 22-FEB-2023

Summary[edit | edit source]

  • organism: Streptococcus pneumoniae AP200
  • locus tag: SPAP_RS09190 [old locus tag: SPAP_1852 ]
  • pan locus tag?: PNEUPAN003378000
  • symbol: SPAP_RS09190
  • pan gene symbol?: nmlR
  • synonym:
  • product: stress response transcriptional regulator NmlR

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SPAP_RS09190 [old locus tag: SPAP_1852 ]
  • symbol: SPAP_RS09190
  • product: stress response transcriptional regulator NmlR
  • replicon: chromosome
  • strand: -
  • coordinates: 1744938..1745291
  • length: 354
  • essential: unknown other strains

Accession numbers[edit | edit source]

  • Location: NC_014494 (1744938..1745291) NCBI
  • BioCyc: SPAP_RS09190 BioCyc
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_1637 PneumoBrowse

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    GTGAATATTAAATCTGCCAGTGATTTGTTGGGAATTTCAGCGGATACGATTCGGTATTAT
    GAACGGGTTGGTCTTGTGCCACCGATTACTCGTACTGCTACTGGGATTCGTGATTTTCAA
    GATCAGGATATCGAAGCGCTGGAATTTATTAAGTGTTTTCGTTCGGCGGGTGTCTCTGTA
    GATAGTTTAGTTGACTATATGTCGCTCTACCAAAAGGGAGATGAAACGAGAGAGGAGAGG
    CTTGGTATTTTAGAAGAGGAAAAGCAAAAATTAGAGGAGCGCTTGTCTCAGCTACAGACA
    GCTTTAAATCGTTTAAATCTCAAAATTAAACTTTATAAGGAAGGAAAATTTTAA
    60
    120
    180
    240
    300
    354

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SPAP_RS09190 [old locus tag: SPAP_1852 ]
  • symbol: SPAP_RS09190
  • description: stress response transcriptional regulator NmlR
  • length: 117
  • theoretical pI: 4.99223
  • theoretical MW: 13463.3
  • GRAVY: -0.45641

Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions DNA interactions Cu(I)-responsive transcriptional regulator (TIGR02044; HMM-score: 65.3)
    and 7 more
    Signal transduction Regulatory functions DNA interactions Zn(II)-responsive transcriptional regulator (TIGR02043; HMM-score: 49.4)
    Cellular processes Cellular processes Detoxification Hg(II)-responsive transcriptional regulator (TIGR02051; HMM-score: 47.2)
    Signal transduction Regulatory functions DNA interactions Hg(II)-responsive transcriptional regulator (TIGR02051; HMM-score: 47.2)
    Signal transduction Regulatory functions DNA interactions Cd(II)/Pb(II)-responsive transcriptional regulator (TIGR02047; HMM-score: 45.1)
    Cellular processes Cellular processes Detoxification mercuric resistence transcriptional repressor protein MerD (TIGR02054; HMM-score: 24)
    Cellular processes Cellular processes Detoxification redox-sensitive transcriptional activator SoxR (TIGR01950; HMM-score: 22.9)
    Signal transduction Regulatory functions DNA interactions redox-sensitive transcriptional activator SoxR (TIGR01950; HMM-score: 22.9)
  • TheSEED: see SPAP_1852
  • PFAM:
    HTH (CL0123) MerR_1; MerR HTH family regulatory protein (PF13411; HMM-score: 58.1)
    MerR; MerR family regulatory protein (PF00376; HMM-score: 49.4)
    and 2 more
    MerR-DNA-bind; MerR, DNA binding (PF09278; HMM-score: 31.5)
    NUMOD1; NUMOD1 domain (PF07453; HMM-score: 15.3)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • genes regulated by NmlR*, TF important in Carbonyl stress response: in TIGR4

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006959
    • TAT(Tat/SPI): 0.000714
    • LIPO(Sec/SPII): 0.000852
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_001022629 NCBI
  • UniProt:

Protein sequence[edit | edit source]

  • MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDIEALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTALNRLNLKIKLYKEGKF

Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

Expression data[edit | edit source]

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]

Uwe H Stroeher, Stephen P Kidd, Sian L Stafford, Michael P Jennings, James C Paton, Alastair G McEwan
A pneumococcal MerR-like regulator and S-nitrosoglutathione reductase are required for systemic virulence.
J Infect Dis: 2007, 196(12);1820-6
[PubMed:18190263] [WorldCat.org] [DOI] (P p)
Adam J Potter, Stephen P Kidd, Alastair G McEwan, James C Paton
The MerR/NmlR family transcription factor of Streptococcus pneumoniae responds to carbonyl stress and modulates hydrogen peroxide production.
J Bacteriol: 2010, 192(15);4063-6
[PubMed:20525825] [WorldCat.org] [DOI] (I p)