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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 02-MAY-2018

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae D39
  • locus tag: SPD_0843 [new locus tag: SPD_RS04535 ]
  • pan locus tag?: PNEUPAN001971000
  • symbol: celA
  • pan gene symbol?: comEA
  • synonym:
  • product: competence protein CelA

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPD_0843 [new locus tag: SPD_RS04535 ]
  • symbol: celA
  • product: competence protein CelA
  • replicon: chromosome
  • strand: +
  • coordinates: 857497..858147
  • length: 651
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGAAGCAATTATCGAGAAAATCAAAGAGTATAAAATCATCGTCATCTGTACTGGTCTG
    GGCTTGCTTGTAGGAGGATTTTTCCTGCTAAAACCAGCTCCACAAACACCTGTCAAAGAG
    ACGAATTTGCAGGCTGAAGTCGCAGCTGTTTCCAAGGATTTGGTATCCGAAAAGGAAGTG
    AACAAGGAAGAAAAGGAAGAACCCCTTGAACAAGATCTAATCACAGTAGATGTCAAAGGT
    GCTGTCAAATCGCCAGGGATTTATGACTTGCCTGTAGGTAGTCGAGTCAATGATGCTGTT
    CAGAAGGCTGGTGGCTTGACAGAGCAAGCAGACAGCAAGTCGCTCAATCTAGCTCAGAAA
    GTTAGTGATGAGGCTCTGGTTTACGTTCCTACTAAGGGAGAAGAAGCAGTTAGCCAACAG
    ACTGGTTTGGGGACAGCTTCTTCAATAAGCAAGGAAAAGAAGGTCAATCTCAACAAGGCC
    AGTCTGGAAGAACTCAAGCAGGTCAAGGGACTGGGAGGAAAACGAGCTCAGGACATTATC
    GACCATCGTGAGGCAAATGGCAAGTTCAAGTCAGTAGACGAGCTCAAGAAGGTCTCTGGC
    ATTGGTGGCAAAACAATAGAAAAGCTTAAAGACTATGTTACAGTGGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    651

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPD_0843 [new locus tag: SPD_RS04535 ]
  • symbol: CelA
  • description: competence protein CelA
  • length: 216
  • theoretical pI: 5.52067
  • theoretical MW: 23229.5
  • GRAVY: -0.342593

⊟Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes DNA transformation comEA protein (TIGR01259; HMM-score: 184.4)
    and 6 more
    Cellular processes Cellular processes DNA transformation competence protein ComEA helix-hairpin-helix repeat region (TIGR00426; HMM-score: 71.6)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, alpha subunit, Gram-positive type (TIGR01405; EC 2.7.7.7; HMM-score: 19)
    Unknown function Enzymes of unknown specificity putative DNA modification/repair radical SAM protein (TIGR03916; HMM-score: 14.3)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair Holliday junction DNA helicase RuvA (TIGR00084; EC 3.6.4.12; HMM-score: 13.9)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, alpha subunit (TIGR00594; EC 2.7.7.7; HMM-score: 13.5)
    polysaccharide export protein EpsE (TIGR03028; HMM-score: 13.1)
  • TheSEED  :
    • Late competence protein ComEA, DNA receptor
    DNA Metabolism DNA uptake, competence Gram Positive Competence  Late competence protein ComEA, DNA receptor
    and 1 more
    DNA Metabolism DNA uptake, competence Late competence  Late competence protein ComEA, DNA receptor
  • PFAM:
    HHH (CL0198) HHH_3; Helix-hairpin-helix motif (PF12836; HMM-score: 84.7)
    and 11 more
    Ubiquitin (CL0072) SLBB; SLBB domain (PF10531; HMM-score: 53.6)
    HHH (CL0198) HHH; Helix-hairpin-helix motif (PF00633; HMM-score: 47.3)
    HHH_5; Helix-hairpin-helix domain (PF14520; HMM-score: 26.8)
    HHH_6; Helix-hairpin-helix motif (PF14579; HMM-score: 24.6)
    HHH_2; Helix-hairpin-helix motif (PF12826; HMM-score: 23.8)
    T2SSK; Type II secretion system (T2SS), protein K (PF03934; HMM-score: 19.7)
    HHH_8; Helix-hairpin-helix domain (PF14716; HMM-score: 17.2)
    PsbU; Photosystem II 12 kDa extrinsic protein (PsbU) (PF06514; HMM-score: 17)
    RNA_pol_A_CTD; Bacterial RNA polymerase, alpha chain C terminal domain (PF03118; HMM-score: 15.8)
    IMS_HHH; IMS family HHH motif (PF11798; HMM-score: 14.1)
    no clan defined UPF0758_N; UPF0758 N-terminal (PF20582; HMM-score: 12.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.17
    • Cytoplasmic Membrane Score: 9.51
    • Cellwall Score: 0.16
    • Extracellular Score: 0.15
    • Internal Helix: 1
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.226741
    • TAT(Tat/SPI): 0.018476
    • LIPO(Sec/SPII): 0.025673
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQAEVAAVSKDLVSEKEVNKEEKEEPLEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQKAGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGLGTASSISKEKKVNLNKASLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGGKTIEKLKDYVTVD

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: ComX1 regulon
    ComX1(sigma factor)controls the expression of late competence genes [1]   other strains

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Jelle Slager, Rieza Aprianto, Jan-Willem Veening
    Refining the Pneumococcal Competence Regulon by RNA Sequencing.
    J Bacteriol: 2019, 201(13);
    [PubMed:30885934] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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