PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BM6001

serotype 19F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7465

serotype 1

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 21-MAR-2025

⊟Summary

• organism: Streptococcus pneumoniae D39
• locus tag: SPD_RS09250 [old locus tag: SPD_1737 ]
• pan locus tag^?: PNEUPAN003528000
• symbol: lytA
• pan gene symbol^?: lytA
• synonym:
• product: N-acetylmuramoyl-L-alanine amidase LytA

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SPD_RS09250 [old locus tag: SPD_1737 ]
• symbol: lytA
• product: N-acetylmuramoyl-L-alanine amidase LytA
• replicon: chromosome
• strand: -
• coordinates: 1729602..1730558
• length: 957
• essential: unknown

⊟Accession numbers

• Location: NC_008533 (1729602..1730558) NCBI
• BioCyc: G1G6V-1892 BioCyc
• MicrobesOnline: see SPD_1737
• PneumoBrowse for strain D39V: SPV_1737 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  
  ATGGAAATTAATGTGAGTAAATTAAGAACAGATTTGCCTCAAGTCGGCGTGCAACCATAT
  AGGCAAGTACACGCACACTCAACTGGGAATCCGCATTCAACCGTACAGAATGAAGCGGAT
  TATCACTGGCGGAAAGACCCAGAATTAGGTTTTTTCTCGCACATTGTTGGGAACGGTTGC
  ATCATGCAGGTAGGACCTGTTGATAATGGTGCCTGGGACGTTGGGGGCGGTTGGAATGCT
  GAGACCTATGCAGCGGTTGAACTGATTGAAAGCCATTCAACCAAAGAAGAGTTCATGACG
  GACTACCGCCTTTATATCGAACTCTTACGCAATCTAGCAGATGAAGCAGGTTTGCCGAAA
  ACGCTTGATACAGGGAGTTTAGCTGGAATTAAAACGCACGAGTATTGCACGAATAACCAA
  CCAAACAACCACTCAGACCACGTTGACCCTTATCCATATCTTGCTAAATGGGGCATTAGC
  CGTGAGCAGTTTAAGCATGATATTGAGAACGGCTTGACGATTGAAACAGGCTGGCAGAAG
  AATGACACTGGCTACTGGTACGTACATTCAGACGGCTCTTATCCAAAAGACAAGTTTGAG
  AAAATCAATGGCACTTGGTACTACTTTGACAGTTCAGGCTATATGCTTGCAGACCGCTGG
  AGGAAGCACACAGACGGCAACTGGTACTGGTTCGACAACTCAGGCGAAATGGCTACAGGC
  TGGAAGAAAATCGCTGATAAGTGGTACTATTTCAACGAAGAAGGTGCCATGAAGACAGGC
  TGGGTCAAGTACAAGGACACTTGGTACTACTTAGACGCTAAAGAAGGCGCCATGGTATCA
  AATGCCTTTATCCAGTCAGCGGACGGAACAGGCTGGTACTACCTCAAACCAGACGGAACA
  CTGGCAGACAGGCCAGAATTCACAGTAGAGCCAGATGGCTTGATTACAGTAAAATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  957
  

⊟Protein

⊟General

• locus tag: SPD_RS09250 [old locus tag: SPD_1737 ]
• symbol: LytA
• description: N-acetylmuramoyl-L-alanine amidase LytA
• length: 318
• theoretical pI: 4.98534
• theoretical MW: 36572.1
• GRAVY: -0.769811

⊟Function

• TIGRFAM:
  glucan-binding repeat (TIGR04035; HMM-score: 56.3)
  and 1 more
  Signal transduction Regulatory functions Protein interactions exported signaling peptide, YydF/SAG_2028 family (TIGR04077; HMM-score: 7.8)
• TheSEED: see SPD_1737
• PFAM:
  Choline_binding (CL0694) Choline_bind_3; Choline-binding repeat (PF19127; HMM-score: 99.1)
  Choline_bind_1; Putative cell wall binding repeat (PF01473; HMM-score: 91)
  and 2 more
  no clan defined Amidase_2; N-acetylmuramoyl-L-alanine amidase (PF01510; HMM-score: 26.1)
  Choline_binding (CL0694) Choline_bind_2; Choline-binding repeat (PF19085; HMM-score: 24.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Extracellular
  • Cytoplasmic Score: 0.1
  • Cytoplasmic Membrane Score: 0.14
  • Cellwall Score: 0.15
  • Extracellular Score: 9.6
  • Internal Helices: 0
• DeepLocPro: Extracellular
  • Cytoplasmic Score: 0.0569
  • Cytoplasmic Membrane Score: 0.0001
  • Cell wall & surface Score: 0.0151
  • Extracellular Score: 0.9279
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.031166
  • TAT(Tat/SPI): 0.002985
  • LIPO(Sec/SPII): 0.00273
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: WP_000405235 NCBI
• UniProt: see SPD_1737

⊟Protein sequence

• MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWRKDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDYRLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGISREQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLADRWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEGAMVSNAFIQSADGTGWYYLKPDGTLADRPEFTVEPDGLITVK

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription

• transcription start site:

⊟Expression data

• PneumoExpress for strain D39V: SPV_1737 PneumoExpress

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications

P García, M Paz González, E García, J L García, R López
The molecular characterization of the first autolytic lysozyme of Streptococcus pneumoniae reveals evolutionary mobile domains.
Mol Microbiol: 1999, 33(1);128-38
[PubMed:10411730] [WorldCat.org] [DOI] (P p)P Balachandran, S K Hollingshead, J C Paton, D E Briles
The autolytic enzyme LytA of Streptococcus pneumoniae is not responsible for releasing pneumolysin.
J Bacteriol: 2001, 183(10);3108-16
[PubMed:11325939] [WorldCat.org] [DOI] (P p)E Díaz, R López, J L García
Role of the major pneumococcal autolysin in the atypical response of a clinical isolate of Streptococcus pneumoniae.
J Bacteriol: 1992, 174(17);5508-15
[PubMed:1355082] [WorldCat.org] [DOI] (P p)Peter Mellroth, Robert Daniels, Alice Eberhardt, Daniel Rönnlund, Hans Blom, Jerker Widengren, Staffan Normark, Birgitta Henriques-Normark
LytA, major autolysin of Streptococcus pneumoniae, requires access to nascent peptidoglycan.
J Biol Chem: 2012, 287(14);11018-29
[PubMed:22334685] [WorldCat.org] [DOI] (I p)J V Höltje, A Tomasz
Specific recognition of choline residues in the cell wall teichoic acid by the N-acetylmuramyl-L-alanine amidase of Pneumococcus.
J Biol Chem: 1975, 250(15);6072-6
[PubMed:238995] [WorldCat.org] (P p)A M Berry, R A Lock, D Hansman, J C Paton
Contribution of autolysin to virulence of Streptococcus pneumoniae.
Infect Immun: 1989, 57(8);2324-30
[PubMed:2568343] [WorldCat.org] [DOI] (P p)J F Garcia-Bustos, A Tomasz
Teichoic acid-containing muropeptides from Streptococcus pneumoniae as substrates for the pneumococcal autolysin.
J Bacteriol: 1987, 169(2);447-53
[PubMed:2879828] [WorldCat.org] [DOI] (P p)A Tomasz, P Moreillon, G Pozzi
Insertional inactivation of the major autolysin gene of Streptococcus pneumoniae.
J Bacteriol: 1988, 170(12);5931-4
[PubMed:2903859] [WorldCat.org] [DOI] (P p)L V Howard, H Gooder
Specificity of the autolysin of Streptococcus (Diplococcus) pneumoniae.
J Bacteriol: 1974, 117(2);796-804
[PubMed:4149515] [WorldCat.org] [DOI] (P p)P Usobiaga, F J Medrano, M Gasset, J L Garciá, J L Saiz, G Rivas, J Laynez, M Menéndez
Structural organization of the major autolysin from Streptococcus pneumoniae.
J Biol Chem: 1996, 271(12);6832-8
[PubMed:8636107] [WorldCat.org] [DOI] (P p)