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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 30-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae G54
  • locus tag: SPG_1637 [new locus tag: SPG_RS08180 ]
  • pan locus tag?: PNEUPAN003140000
  • symbol: SPG_1637
  • pan gene symbol?: stkP
  • synonym: pknB
  • product: serine/threonine protein kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPG_1637 [new locus tag: SPG_RS08180 ]
  • symbol: SPG_1637
  • product: serine/threonine protein kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 1583110..1584741
  • length: 1632
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGGACAAATTCTCTTGGCTATGCGCTTGGCCCATACTCGAGGAATTGTTCACAGGGAC
    TTGAAACCTCAAAATATCCTTTTGACACCAGATGGGACGGCCAAGGTCACAGACTTTGGG
    ATTGCTGTAGCCTTTGCAGAGACAAGTCTGACCCAGACTAACTCGATGTTGGGCTCAGTT
    CATTACTTGTCACCAGAGCAGGCGCGTGGTTCTAAGGCGACTGTGCAGAGTGATATCTAT
    GCCATGGGGATTATTTTCTATGAGATGTTGACAGGCCATATCCCTTATGACGGGGATAGC
    GCGGTGACCATTGCCCTCCAGCATTTCCAGAAACCCCTGCCGTCCGTTATTGCAGAAAAT
    CCATCTGTACCTCAGGCTTTAGAAAATGTTATTATCAAGGCAACTGCTAAAAAGTTGACC
    AATCGCTACCGCTCGGTTTCAGAGATGTATGTGGACTTGTCTAGTAGCTTGTCCTACAAT
    CGTAGAAATGAAAGTAAGTTAATCTTTGATGAAACGAGCAAGGCAGATACCAAGACCTTG
    CCGAAGGTTTCTCAGAGTACCTTGACATCTATTCCTAAGGTTCAAGCGCAGACAGAACAC
    AAATCAATCAAAAACCCAAGCCAGGCTGTGACAGAGGAAACTTACCAACCACAAGCACCG
    AAAAAACATAGATTTAAGATGCGTTACCTGATTTTGTTGGCCAGCCTTGTATTGGTGGCA
    GCTTCTCTTATTTGGATACTATCCAGAACTCCTGCAACCATTGCCATTCCAGATGTGGCA
    GGTCAGACAGTTGCAGAGGCCAAGGCAACGCTCAAAAAAGCCAATTTTGAGATTGGTGAG
    GAGAAGACAGAGGCTAGTGAAAAGGTGGAAGAAGGGCGGATTATCCGTACAGATCCTGGC
    GCTGGAACTGGTCGAAAAGAAGGAACGAAAATCAATCTGGTTGTCTCATCAGGCAAACAA
    TCCTTCCAAATTAGTAATTATGTCGGCCGGAAATCTTCTGATGTTATCGCGGAATTAAAA
    GAGAAAAAAGTTCCAGATAATTTGATTAAAATTGAGGAAGAAGAGTCGAATGAGAGTGAG
    GCTGGAACGGTCCTGAAGCAAAGTCTACCAGAAGGTACGACCTATGACTTGAGCAAGGCA
    ACTCAAATTGTTTTGACAGTAGCTAAAAAAGCTACGACGATTCAATTAGGGAACTATATT
    GGACGGAACTCTACAGAAGTAATCTCAGAACTCAAGCAGAAGAAGGTTCCTGAGAATTTG
    ATTAAGATAGAGGAAGAAGAGTCCAGCGAAAGCGAACCAGGAACGATTATGAAACAAAGT
    CCAGGTGCCGGAACGACTTATGATGTGAGTAAACCTACTCAAATTGTCTTGACAGTAGCT
    AAAAAAGTTACAAGTGTTGCCATGCCGAGTTACATTGGTTCCAGCTTGGAGTTTACTAAG
    AACAATTTGATTCAAATTGTTGGGATTAAGGAAGCTAATATAGAAGTTGTAGAAGTGACG
    ACAGCGCCTGCAGGTAGTGCAGAAGGCATGGTTGTTGAACAAAGTCCTAGAGCAGGTGAA
    AAGGTAGACCTCAATAAGACTAGAGTCAAGATTTCAATCTACAAACCTAAAACAACTTCA
    GCTACTCCTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1632

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPG_1637 [new locus tag: SPG_RS08180 ]
  • symbol: SPG_1637
  • description: serine/threonine protein kinase
  • length: 543
  • theoretical pI: 9.4768
  • theoretical MW: 59073.1
  • GRAVY: -0.30442

⊟Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Toxin production and resistance TOMM system kinase/cyclase fusion protein (TIGR03903; HMM-score: 105.3)
    and 1 more
    Unknown function General Kae1-associated kinase Bud32 (TIGR03724; EC 2.7.-.-; HMM-score: 17.1)
  • TheSEED  :
    • Serine/threonine protein kinase PrkC, regulator of stationary phase
    A Gram-positive cluster that relates ribosomal protein L28P to a set of uncharacterized proteins  Serine/threonine protein kinase PrkC, regulator of stationary phase
    and 1 more
    Conserved gene cluster associated with Met-tRNA formyltransferase  Serine/threonine protein kinase PrkC, regulator of stationary phase
  • PFAM:
    no clan defined PASTA; PASTA domain (PF03793; HMM-score: 148.4)
    and 6 more
    PKinase (CL0016) Pkinase; Protein kinase domain (PF00069; HMM-score: 100.4)
    PK_Tyr_Ser-Thr; Protein tyrosine and serine/threonine kinase (PF07714; HMM-score: 72.1)
    Kinase-like; Kinase-like (PF14531; HMM-score: 19.5)
    APH; Phosphotransferase enzyme family (PF01636; HMM-score: 17.3)
    ABC1; ABC1 atypical kinase-like domain (PF03109; HMM-score: 12.9)
    Pkinase_fungal; Fungal protein kinase (PF17667; HMM-score: 12.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.04
    • Cytoplasmic Membrane Score: 9.96
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helix: 1
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.009573
    • TAT(Tat/SPI): 0.000933
    • LIPO(Sec/SPII): 0.000983
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: ACF55178 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVAFAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAVTIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYNRRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQAPKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANFEIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSDVIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKATTIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVSKPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSAEGMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Linda Nováková, Lenka Sasková, Petra Pallová, Jirí Janecek, Jana Novotná, Ales Ulrych, Jose Echenique, Marie-Claude Trombe, Pavel Branny
Characterization of a eukaryotic type serine/threonine protein kinase and protein phosphatase of Streptococcus pneumoniae and identification of kinase substrates.
FEBS J: 2005, 272(5);1243-54
[PubMed:15720398] [WorldCat.org] [DOI] (P p)Lenka Sasková, Linda Nováková, Marek Basler, Pavel Branny
Eukaryotic-type serine/threonine protein kinase StkP is a global regulator of gene expression in Streptococcus pneumoniae.
J Bacteriol: 2007, 189(11);4168-79
[PubMed:17416671] [WorldCat.org] [DOI] (P p)Andrew T Ulijasz, Shaun P Falk, Bernard Weisblum
Phosphorylation of the RitR DNA-binding domain by a Ser-Thr phosphokinase: implications for global gene regulation in the streptococci.
Mol Microbiol: 2009, 71(2);382-90
[PubMed:19040630] [WorldCat.org] [DOI] (I p)Makoto Osaki, Tania Arcondéguy, Amandine Bastide, Christian Touriol, Hervé Prats, Marie-Claude Trombe
The StkP/PhpP signaling couple in Streptococcus pneumoniae: cellular organization and physiological characterization.
J Bacteriol: 2009, 191(15);4943-50
[PubMed:19502404] [WorldCat.org] [DOI] (I p)Linda Nováková, Silvia Bezousková, Petr Pompach, Petra Spidlová, Lenka Sasková, Jaroslav Weiser, Pavel Branny
Identification of multiple substrates of the StkP Ser/Thr protein kinase in Streptococcus pneumoniae.
J Bacteriol: 2010, 192(14);3629-38
[PubMed:20453092] [WorldCat.org] [DOI] (I p)Katrin Beilharz, Linda Nováková, Daniela Fadda, Pavel Branny, Orietta Massidda, Jan-Willem Veening
Control of cell division in Streptococcus pneumoniae by the conserved Ser/Thr protein kinase StkP.
Proc Natl Acad Sci U S A: 2012, 109(15);E905-13
[PubMed:22431591] [WorldCat.org] [DOI] (I p)Aurore Fleurie, Sylvie Manuse, Chao Zhao, Nathalie Campo, Caroline Cluzel, Jean-Pierre Lavergne, Céline Freton, Christophe Combet, Sébastien Guiral, Boumediene Soufi, Boris Macek, Erkin Kuru, Michael S VanNieuwenhze, Yves V Brun, Anne-Marie Di Guilmi, Jean-Pierre Claverys, Anne Galinier, Christophe Grangeasse
Interplay of the serine/threonine-kinase StkP and the paralogs DivIVA and GpsB in pneumococcal cell elongation and division.
PLoS Genet: 2014, 10(4);e1004275
[PubMed:24722178] [WorldCat.org] [DOI] (I e)Jenny A Herbert, Andrea M Mitchell, Timothy J Mitchell
A Serine-Threonine Kinase (StkP) Regulates Expression of the Pneumococcal Pilus and Modulates Bacterial Adherence to Human Epithelial and Endothelial Cells In Vitro.
PLoS One: 2015, 10(6);e0127212
[PubMed:26090876] [WorldCat.org] [DOI] (I e)

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