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70585
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HU-OH
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Hu15
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Hu17
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INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
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OXC141
serotype 3
P1031
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R6
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SP49
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SPN032672
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SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
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ST556
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TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 31-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae JJA
  • locus tag: SPJ_0687 [new locus tag: SPJ_RS03435 ]
  • pan locus tag?: PNEUPAN001690000
  • symbol: SPJ_0687
  • pan gene symbol?: livJ
  • synonym:
  • product: ABC transporter substrate-binding protein - branched chain amino acid transport

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPJ_0687 [new locus tag: SPJ_RS03435 ]
  • symbol: SPJ_0687
  • product: ABC transporter substrate-binding protein - branched chain amino acid transport
  • replicon: chromosome
  • strand: +
  • coordinates: 662799..663959
  • length: 1161
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAAGAAAAAATTTGCCCTATCGTTTGTGGCGCTTGCAAGTGTAGCACTTCTTGCAGCC
    TGTGGAGAAGTGAAGTCTGGAGCAGTCAACACTGCTGGTAACTCAGTAGAGGAAAAGACA
    ATTAAAATCGGGTTTAACTTTGAAGAATCAGGTTCTTTAGCTGCATACGGAACAGCTGAA
    CAAAAAGGTGCCCAATTGGCTGTTGATGAAATCAATGCCGCAGGTGGTATCGATGGAAAA
    CAAATCGAAGTAGTCGATAAAGATAATAAGTCTGAAACAGCTGAGGCTGCTTCAGTTACA
    ACTAACCTTGTAACCCAATCTAAAGTATCAGCAGTCGTAGGACCTGCAACATCTGGTGCG
    ACTGCAGCTGCGGTAGCGAACGCTACAAAAGCAGGTGTTCCCTTGATCTCACCAAGTGCG
    ACTCAAGATGGATTGACTAAAGGTCAAGATTACCTCTTTATTGGAACTTTCCAAGATAGC
    TTCCAAGGAAAAATTATCTCAAACTATGTTTCTGAAAAATTAAATGCTAAGAAAGTTGTT
    CTTTACACTGACAATGCCAGTGACTATGCTAAAGGGATTGCCAAATCTTTCCGCGAGTCA
    TACAAGGGTGAAATCGTTGCAGATGAAACTTTCGTAGCAGGTGACACAGACTTCCAAGCA
    GCCCTTACAAAAATGAAAGGGAAAGACTTTGATGCTATCGTTGTTCCTGGTTACTATAAT
    GAGGCTGGTAAGATTGTAAACCAAGCGCGTGGTATGGGAATTGACAAACCAATCGTTGGT
    GGTGATGGATTCAACGGTGAGGAGTTTGTACAACAAGCAACTGCTGAAAAAGCATCAAAC
    ATCTACTTTATCTCAGGCTTCTCAACTACTGTAGAAGTTTCAGCTAAAGCTAAAGCCTTC
    CTTGACGCTTACCGTGCTAAGTACAATGAAGAGCCTTCAACATTTGCAGCCTTGGCTTAT
    GATTCAGTTCACCTTGTAGCAAACGCAGCAAAAGGTGCTAAAAATTCAGGTGAAATCAAG
    GATAACCTTGCTAAAACAAAAGATTTTGAAGGTGTAACTGGTCAAACAAGCTTCGATGCA
    GACCACAACACAGTCAAAACTGCTTACATGATGACCATGAACAATGGTAAAGTTGAAGCA
    GCAGAAGTTGTAAAACCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1161

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPJ_0687 [new locus tag: SPJ_RS03435 ]
  • symbol: SPJ_0687
  • description: ABC transporter substrate-binding protein - branched chain amino acid transport
  • length: 386
  • theoretical pI: 4.93857
  • theoretical MW: 40415
  • GRAVY: -0.17772

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Amino acids, peptides and amines urea ABC transporter, substrate-binding protein (TIGR03669; HMM-score: 73.4)
    Metabolism Transport and binding proteins Amino acids, peptides and amines urea ABC transporter, urea binding protein (TIGR03407; HMM-score: 63.3)
  • TheSEED  :
    • High-affinity leucine-specific transport system, periplasmic binding protein LivK (TC 3.A.1.4.1)
    Membrane Transport ABC transporters ABC transporter branched-chain amino acid (TC 3.A.1.4.1)  Branched-chain amino acid ABC transporter, amino acid-binding protein (TC 3.A.1.4.1)
    and 1 more
    Membrane Transport ABC transporters ABC transporter branched-chain amino acid (TC 3.A.1.4.1)  High-affinity leucine-specific transport system, periplasmic binding protein LivK (TC 3.A.1.4.1)
  • PFAM:
    Periplas_BP (CL0144) Peripla_BP_6; Periplasmic binding protein (PF13458; HMM-score: 200.9)
    and 3 more
    ANF_receptor; Receptor family ligand binding region (PF01094; HMM-score: 103.3)
    Peripla_BP_5; Periplasmic binding protein domain (PF13433; HMM-score: 76.6)
    LppaM (CL0421) LPAM_2; Prokaryotic lipoprotein-attachment site (PF13627; HMM-score: 8.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helix: 1
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.0002
    • Cytoplasmic Membrane Score: 0.7768
    • Cell wall & surface Score: 0.0087
    • Extracellular Score: 0.2144
  • SignalP: Signal peptide LIPO(Sec/SPII) length 20 aa
    • SP(Sec/SPI): 0.003975
    • TAT(Tat/SPI): 0.000077
    • LIPO(Sec/SPII): 0.995755
    • Cleavage Site: CS pos: 20-21. LAA-CG. Pr: 0.9963
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIGFNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTNLVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDSFQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDFQAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAEKASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAKNSGEIKDNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY (repression) regulon
    CodY(TF)important in Amino acid metabolism; RegPrecise 

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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