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serotype 4
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D39V
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Hungary19A-6
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EF3030
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670-6B
serotype 6B
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70585
serotype 5
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A66
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AP200
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HU-OH
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Hu15
serotype 19A
Hu17
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INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 31-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae JJA
  • locus tag: SPJ_1308 [new locus tag: SPJ_RS06495 ]
  • pan locus tag?: PNEUPAN002684000
  • symbol: SPJ_1308
  • pan gene symbol?: hemN
  • synonym: hemW
  • product: putative oxygen-independent coproporphyrinogen III oxidase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPJ_1308 [new locus tag: SPJ_RS06495 ]
  • symbol: SPJ_1308
  • product: putative oxygen-independent coproporphyrinogen III oxidase
  • replicon: chromosome
  • strand: -
  • coordinates: 1253877..1255007
  • length: 1131
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGCAGAAAAAACCAACGTCAGCCTATGTGCACATCCCATTTTGTACCCAGATTTGTTAT
    TATTGTGATTTTTCAAAGGTCTTCATCAAAAATCAGCCAGTCGACAGCTATTTAGAGCAT
    CTGCTGGAAGAGTTTCGTTCTTATGATATTGAAAAGTTGTCAACCCTTTATATCGGTGGT
    GGAACACCGACAGCCCTGTCGGCTCCGCAACTGGAGGTGTTACTGAATGGCTTGACTAAA
    AACTTGGACTTGTCTGTCTTGGAAGAGTTGACCATTGAAGCCAATCCAGGCGATTTGGAT
    GCGGATAAGATAGCTGTTTTGAAAAATTCGGCTGTCAATCGTGTTTCGCTAGGTGTCCAG
    ACCTTTGATGATAAGATGTTGAAAAAGATTGGGCGCAGTCATTTGGAGAAGGATATTTAT
    GAAAATATCGATCGTCTGAAACTGGCTGGTTTTGACAACATCTCCATTGATTTGATTTAT
    GCTCTGCCTGGTCAGACCATGGAGCAAGTAAAGGAAAATGTGGCTAAAGCCATTGGACTG
    GATATTCCCCACATGAGTTTGTATAGTTTGATTTTAGAAAACCATACGGTCTTTATGAAC
    CGGATGCGACGTGGGAAATTGCCTCTGCCTAAGGAGGAACTAGAAGCGGAGATGTTTGAG
    TACATCATTGCAGAGCTGGAGCGAGCGGGTTTTGAGCATTATGAGATTTCTAATTTCTCC
    AAACCCGGTTTTGAAAGTCGTCACAATCTCATGTACTGGGACAATGCTGAATACTATGGT
    ATTGGTGCAGGGGCATCTGGTTATGTCAATGGAGTACGCTATAAAAATCATGGTCCCATT
    CGTCATTATCTCAGTGCGGTTGAGGAAGGCAATGCTTGTATTACAGAAGATCACCTGAGT
    CAAAAGGAGCAAATGGAAGAAGAAATGTTCTTGGGACTCCGCAAGAAATCCGGGGTTTCC
    ATGGCGCGATTTGAGGAAAAATTTGGACAGTCTTTTGCTGGACTTTATGGAGAAATTGTC
    AGAGATTTGGTTCAACAAGGCCTCATGCAGATTGAGGGTGACCACGTTCGCATGACAAAG
    AGAGGTCTCTTTTTGGGAGATACTGTAGCAGAACGATTTATTTTGGAGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1131

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPJ_1308 [new locus tag: SPJ_RS06495 ]
  • symbol: SPJ_1308
  • description: putative oxygen-independent coproporphyrinogen III oxidase
  • length: 376
  • theoretical pI: 5.10946
  • theoretical MW: 42748.6
  • GRAVY: -0.292819

⊟Function[edit | edit source]

  • reaction:
    EC 1.3.99.22?  ExPASy
    Transferred entry: 1.3.98.3
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin putative oxygen-independent coproporphyrinogen III oxidase (TIGR00539; EC 1.3.99.22; HMM-score: 379)
    and 16 more
    putative heme utilization radical SAM enzyme HutW (TIGR04107; HMM-score: 224.6)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin oxygen-independent coproporphyrinogen III oxidase (TIGR00538; EC 1.3.99.22; HMM-score: 185.4)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin coproporphyrinogen dehydrogenase HemZ (TIGR03994; EC 1.3.99.22; HMM-score: 173)
    HemN-related non-iron pseudo-SAM protein PsgB (TIGR04419; HMM-score: 114.5)
    Unknown function Enzymes of unknown specificity radical SAM protein, TIGR01212 family (TIGR01212; HMM-score: 38.7)
    Unknown function Enzymes of unknown specificity B12-binding domain/radical SAM domain protein, MJ_1487 family (TIGR04013; HMM-score: 37.1)
    Genetic information processing Transcription DNA-dependent RNA polymerase radical SAM enzyme/protein acetyltransferase, ELP3 family (TIGR01211; EC 2.3.1.48; HMM-score: 25.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Molybdopterin molybdenum cofactor biosynthesis protein A (TIGR02666; HMM-score: 23.9)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA-i(6)A37 thiotransferase enzyme MiaB (TIGR01574; EC 2.-.-.-; HMM-score: 22.8)
    Genetic information processing Protein synthesis tRNA and rRNA base modification MiaB-like tRNA modifying enzyme (TIGR01579; HMM-score: 21.6)
    radical SAM methylthiotransferase, MiaB/RimO family (TIGR00089; EC 2.1.1.-,2.8.1.-; HMM-score: 20.6)
    Cellular processes Cellular processes Toxin production and resistance radical SAM P-methyltransferase, PhpK family (TIGR04479; EC 2.1.-.-; HMM-score: 17.5)
    Unknown function Enzymes of unknown specificity B12-binding domain/radical SAM domain protein, MJ_0865 family (TIGR04014; HMM-score: 13.5)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Molybdopterin probable molybdenum cofactor biosynthesis protein A (TIGR02668; HMM-score: 13.4)
    Genetic information processing Protein fate Protein modification and repair anaerobic ribonucleoside-triphosphate reductase activating protein (TIGR02495; EC 1.97.-.-; HMM-score: 12.9)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides 2'-Deoxyribonucleotide metabolism anaerobic ribonucleoside-triphosphate reductase activating protein (TIGR02495; EC 1.97.-.-; HMM-score: 12.9)
  • TheSEED  :
    • Heme chaperone HemW, insertion of heme into hemoproteins
    Cofactors, Vitamins, Prosthetic Groups, Pigments Tetrapyrroles CPO analysis  Hypothetical radical SAM family enzyme in heat shock gene cluster, similarity with CPO of BS HemN-type
    and 1 more
    Stress Response Heat shock Heat shock dnaK gene cluster extended  Hypothetical radical SAM family enzyme in heat shock gene cluster, similarity with CPO of BS HemN-type
  • PFAM:
    TIM_barrel (CL0036) Radical_SAM; Radical SAM superfamily (PF04055; HMM-score: 79.7)
    HTH (CL0123) HemN_C; HemN C-terminal domain (PF06969; HMM-score: 72.7)
    and 1 more
    TPR (CL0020) TAF6_C; TAF6 C-terminal HEAT repeat domain (PF07571; HMM-score: 12.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9984
    • Cytoplasmic Membrane Score: 0.0002
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.0013
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.019578
    • TAT(Tat/SPI): 0.000422
    • LIPO(Sec/SPII): 0.020209
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEEFRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDADKIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIYALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEMFEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKNHGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAGLYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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