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INV200
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JJA
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MDRSPN001
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NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
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SP49
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SPN032672
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SPN034156
serotype 3
SPN034183
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SPN994038
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SPNA45
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Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 31-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae JJA
  • locus tag: SPJ_1568 [new locus tag: SPJ_RS07780 ]
  • pan locus tag?: PNEUPAN003089000
  • symbol: SPJ_1568
  • pan gene symbol?: pbp2b
  • synonym:
  • product: penicillin-binding protein 2B

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPJ_1568 [new locus tag: SPJ_RS07780 ]
  • symbol: SPJ_1568
  • product: penicillin-binding protein 2B
  • replicon: chromosome
  • strand: -
  • coordinates: 1487500..1489542
  • length: 2043
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAGAAAATTTAACAGCCATTCGATTCCGATTCGGCTTAATTTATTGTTTTCAATCGTC
    ATTTTACTCTTTATGACCATTATTGGTCGTTTGTTGTATATGCAGGTTTTGAACAAGGAT
    TTTTATGAAAAAAAGCTAGCCTCAGCTAGTCAGACCAAGGTCACAACCAGTTCTGCCCGT
    GGGGAAATTTATGATGCTAGTGGAAAACCTTTGGTAGAAAATACGTTAAAGCAGGTTGTT
    TCCTTTACGCGTAGCAATAAAATGACGGCTACAGACTTAAAAGAAATAGCTAAAAAGTTA
    CTGACTTATGTGAGCATCAGTTCGCTAAATTTGACAGAACGCCAGCTGGCTGATTACTAT
    TTGGCTGATCCTGAAATCTATAAAAAAACAGTGGAAGCTCTCCCAAGTGAGAAACGATTG
    GATTCAGATGGCAATCGCCTATCCGAATCAGAACTGTATAACAATGCGGTCGATAGTGTC
    CCAACAAGTCAACTAAACTATACAGAGGATGAAAAGAAAGAAATCTATCTTTTTAGTCAG
    TTAAATGCTGTTGGAAACTTTGCGACAGGAACCATTGTGACAGATGCTCTAAATGATTCT
    CAGGTGGCTGTTATTGCCTCTATTTCAAAGGAGATGCCTGGCATTAGTATTTCTACTTCT
    TGGGATCGAAAGATTTTGGAAACTTCCCTTTCTTCTATAGTAGGGAGTGTATCCAGTGAA
    AAAGCCGGTCTCCCAGCGGAAGAAGCAGAAGCCTATCTTAAAAAAGGCTATTCTCTAAAT
    GACCGTGTTGGAACCTCCTATTTGGAAAAGCAATATGAAGAGACCTTACAAGGGAAACGC
    TCGGTAAAAGAAATCCATCTGGATAAATATGGCAATATGGAAAGCGTGGATACAATTGAG
    GAAGGTAGTAAGGGAAACAATATTAAACTGACCATTGATTTGGCCTTCCAAGATAGCGTG
    GATTCTCTGCTGAAAAGTTATTTCAATTCAGAGTTGGGGAATGGTGGAGCTAAGTATTCT
    GAAGGTGTGTATGCAGTCGCCCTTAATCCAAAAACAGGTGCTGTGTTATCCATGTCAGGG
    ATCAAACATGACCTGAAAACGGGAGAGTTGACGCCAGATTCCTTGGGAACGGTAACCAAT
    GTCTTTGTCCCAGGTTCGGTTGTCAAGGCGGCGACCATCAGCTCTGGTTGGGAAAATGGA
    GTCTTGTCAGGAAACCAGACCTTGACAGACCAGTCCATTGTCTTTCAAGGTTCAGCTCCA
    ATTAATTCTTGGTATACTGCTTTTTCTAGGCCAATGCCGATTACGGCAGTTCAGGCTCTA
    GAGTATTCATCCAATGCTTATATGGTCCAAACAGCTCTAGGTCTTATGGGGCAGACCTAT
    CAACCAAATATGTTTGTCGGTACCAGCAATCTAGAGTCTGCTATGGGGAAACTGCGTTCA
    ACCTTTGGCGAATATGGCTTGGGGTCTGCGACTGGGATTGACCTACCAGATGAATCTACT
    GGATTTATTCCCAAAGAGTATAGCTTTGCTAATTACATTACTAATGCCTTTGGGCAGTTT
    GATAACTATACGCCCATGCAGTTGGCTCAGTATGTAGCAACTATTGCAAATGATGGTGTT
    CGTGTGGCTCCTCGTATTGTGGAAGGCATTTATGGCAATAATGATAAGGGAGGACTGGGT
    GACCTGATTCAGCAACTGCAACCGACAGAGATGAATAAGGTCAATATATCCGACTCCGAT
    ATGAGCATCTTGCACCAAGGTTTTTATCAGGTTGCTCATGGGACTAGCGGATTGACAACT
    GGACGTGCCTTTTCAAATGGTGCCTTGGTATCCATTAGCGGAAAAACGGGTACAGCCGAA
    AGCTATGTGGCAGATGGCCAGCAAGCAACTAATACTAATGCGGTGGCCTATGCCCCATCT
    GATAATCCCCAAATCGCTGTTGCAGTGGTCTTTCCTCATAATACCAATCTAACAAATGGT
    GTAGGACCTTCCATTGCGCGTGACATTATCAATCTGTATCAAAAATACCATCCAATGAAC
    TAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2043

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPJ_1568 [new locus tag: SPJ_RS07780 ]
  • symbol: SPJ_1568
  • description: penicillin-binding protein 2B
  • length: 680
  • theoretical pI: 4.96114
  • theoretical MW: 73752.4
  • GRAVY: -0.225735

⊟Function[edit | edit source]

  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan penicillin-binding protein 2 (TIGR03423; HMM-score: 219.6)
    and 3 more
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan stage V sporulation protein D (TIGR02214; HMM-score: 119.9)
    Cellular processes Cellular processes Sporulation and germination stage V sporulation protein D (TIGR02214; HMM-score: 119.9)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan penicillin-binding protein, 1A family (TIGR02074; HMM-score: 45.2)
  • TheSEED  :
    • Cell division protein FtsI [Peptidoglycan synthetase] (EC 2.4.1.129)
    Cell Division and Cell Cycle Cell Division and Cell Cycle - no subcategory Bacterial Cytoskeleton  Cell division protein FtsI [Peptidoglycan synthetase] (EC 2.4.1.129)
    and 1 more
    Cell Wall and Capsule Cell Wall and Capsule - no subcategory Peptidoglycan Biosynthesis  Cell division protein FtsI [Peptidoglycan synthetase] (EC 2.4.1.129)
  • PFAM:
    Beta-lactamase (CL0013) Transpeptidase; Penicillin binding protein transpeptidase domain (PF00905; HMM-score: 261.2)
    and 3 more
    no clan defined PBP_dimer; Penicillin-binding Protein dimerisation domain (PF03717; HMM-score: 120.5)
    DUF6592; Family of unknown function (DUF6592) (PF20235; HMM-score: 14.4)
    ABC-2 (CL0181) ABC_export; Putative ABC exporter (PF16962; HMM-score: 11.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.17
    • Cytoplasmic Membrane Score: 9.51
    • Cellwall Score: 0.16
    • Extracellular Score: 0.15
    • Internal Helix: 1
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.9788
    • Cell wall & surface Score: 0.0002
    • Extracellular Score: 0.021
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.097478
    • TAT(Tat/SPI): 0.000751
    • LIPO(Sec/SPII): 0.013883
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNKDFYEKKLASASQTKVTTSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKEIAKKLLTYVSISSLNLTERQLADYYLADPEIYKKTVEALPSEKRLDSDGNRLSESELYNNAVDSVPTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIVTDALNDSQVAVIASISKEMPGISISTSWDRKILETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQYEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDSLLKSYFNSELGNGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSVVKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTAFSRPMPITAVQALEYSSNAYMVQTALGLMGQTYQPNMFVGTSNLESAMGKLRSTFGEYGLGSATGIDLPDESTGFIPKEYSFANYITNAFGQFDNYTPMQLAQYVATIANDGVRVAPRIVEGIYGNNDKGGLGDLIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTAESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYHPMN

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription[edit | edit source]

  • transcription start site:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

R Hakenbeck, C Martin, C Dowson, T Grebe
Penicillin-binding protein 2b of Streptococcus pneumoniae in piperacillin-resistant laboratory mutants.
J Bacteriol: 1994, 176(17);5574-7
[PubMed:8071243] [WorldCat.org] [DOI] (P p)

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