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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 11-FEB-2025

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae SPN032672
  • locus tag: SPN032672_RS09740
  • pan locus tag?: PNEUPAN001634000
  • symbol: lysS
  • pan gene symbol?: lysS
  • synonym:
  • product: lysine--tRNA ligase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPN032672_RS09740
  • symbol: lysS
  • product: lysine--tRNA ligase
  • replicon: chromosome
  • strand: +
  • coordinates: 1827982..1829472
  • length: 1491
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_021003 (1827982..1829472) NCBI
  • BioCyc:
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_0620 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGTCTACAGAACATATGGAAGAACTAAATGACCAGCAGATCGTTCGCCGTGAAAAAATG
    GCTGCGCTCCGCGAACAAGGAATCGATCCTTTCGGAAAACGTTTTGAACGTACTGCAAAT
    TCACAAGAATTAAAAGATAAATATGCCAACCTCGATAAAGAACAATTACACGATAAAAAC
    GAAACAGCTACTATCGCAGGACGCTTGATAACCAAACGTGGTAAAGGAAAAGTTGGTTTT
    GCCCACCTTCAAGACCGCGAAGGCCAGATTCAGATCTACGTTCGTAAGGATGCTGTCGGT
    GAAGAAAACTACGAAATCTTCAAAAAAGCAGACCTTGGTGACTTCCTTGGTGTCGAAGGT
    GAAGTGATGCGTACGGATATGGGAGAACTCTCTATCAAGGCAACCCACATCACACACTTG
    TCTAAGGCTCTTCGTCCTCTTCCTGAGAAATTCCATGGTTTGACAGACGTTGAAACAATT
    TACCGTAAACGTTACCTTGACTTGATTTCTAATCGTGAAAGCTTTGAACGCTTTGTCACT
    CGTTCAAAAATCATCTCTGAAATCCGTCGTTACCTTGACCAAAAAGGATTCCTTGAAGTG
    GAAACACCTGTTCTTCATAATGAAGCCGGTGGTGCTGCTGCCCGTCCATTTATCACCCAC
    CACAATGCCCAAAACATTGACATGGTGCTTCGTATCGCGACTGAGCTTCACTTGAAACGC
    CTTATCGTGGGTGGTATGGAACGTGTCTATGAAATTGGCCGTATCTTCCGTAACGAAGGA
    ATGGACGCTACTCATAACCCTGAGTTCACTTCTATCGAAGTTTACCAAGCTTATGCAGAC
    TTCCAAGACATCATGGACTTGACTGAAGGCATTATCCAACACGCTGCTAAATCAGTCAAA
    GGTGATGGCCCAGTCAACTACCAAGGTACTGAAATCAAGATTAACGAACCATTTAAGCGT
    GTTCATATGGTGGATGCTATCAGAGAAATTACTGGTGTCGATTTCTGGCAAGACATGACT
    TTGGAAGAAGCTAAAACTATCGCTGCTGAGAAGAAAGTTCCAGTTGAGAAACACTACACT
    GAGGTTGGTCACATCATCAATGCCTTCTTTGAAGAGTTTGTTGAAGAAACTTTAATCCAA
    CCAACCTTTGTCTATGGACATCCAGTAGCTGTATCTCCACTCGCTAAGAAAAATCCTGAA
    GACCAACGCTTTACTGACCGTTTCGAGCTCTTTATCATGACTAAGGAGTACGGTAATGCC
    TTTACTGAGTTGAACGACCCAATCGACCAACTTAGCCGTTTTGAAGCCCAAGCTAAAGCC
    AAAGAACTTGGTGATGATGAAGCGACAGGCATCGACTATGACTACATTGAAGCTCTTGAA
    TACGGTATGCCACCAACAGGTGGTTTGGGAATCGGTATCGACCGTCTCTGCATGCTCCTC
    ACTGATACAACAACTATCCGTGATGTATTGCTCTTCCCAACAATGAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1491

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPN032672_RS09740
  • symbol: LysS
  • description: lysine--tRNA ligase
  • length: 496
  • theoretical pI: 5.17507
  • theoretical MW: 56725
  • GRAVY: -0.487702

⊟Function[edit | edit source]

  • reaction:
    EC 6.1.1.6?  ExPASy
    Lysine--tRNA ligase ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA aminoacylation lysine--tRNA ligase (TIGR00499; EC 6.1.1.6; HMM-score: 700.4)
    and 6 more
    Unknown function General EF-P lysine aminoacylase GenX (TIGR00462; HMM-score: 216.6)
    Genetic information processing Protein synthesis tRNA aminoacylation aspartate--tRNA ligase (TIGR00459; EC 6.1.1.-; HMM-score: 168.5)
    Genetic information processing Protein synthesis tRNA aminoacylation aspartate--tRNA(Asn) ligase (TIGR00458; EC 6.1.1.23; HMM-score: 152.9)
    Genetic information processing Protein synthesis tRNA aminoacylation asparagine--tRNA ligase (TIGR00457; EC 6.1.1.22; HMM-score: 110.4)
    Genetic information processing Protein synthesis tRNA aminoacylation phenylalanine--tRNA ligase, alpha subunit (TIGR00468; EC 6.1.1.20; HMM-score: 30.3)
    Genetic information processing Protein synthesis tRNA aminoacylation pyrrolysine--tRNA ligase, C-terminal region (TIGR02367; EC 6.1.1.26; HMM-score: 22.4)
  • TheSEED: data available for D39, Hungary19A-6, TIGR4
  • PFAM:
    tRNA_synt_II (CL0040) tRNA-synt_2; tRNA synthetases class II (D, K and N) (PF00152; HMM-score: 270.9)
    and 3 more
    OB (CL0021) tRNA_anti-codon; OB-fold nucleic acid binding domain (PF01336; HMM-score: 57.4)
    tRNA_synt_II (CL0040) tRNA-synt_2d; tRNA synthetases class II core domain (F) (PF01409; HMM-score: 32.6)
    NADP_Rossmann (CL0063) Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 15.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 10
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9909
    • Cytoplasmic Membrane Score: 0.0001
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.009
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007979
    • TAT(Tat/SPI): 0.002924
    • LIPO(Sec/SPII): 0.003408
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_000102457 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQELKDKYANLDKEQLHDKNETATIAGRLITKRGKGKVGFAHLQDREGQIQIYVRKDAVGEENYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETIYRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFITHHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQAYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVDFWQDMTLEEAKTIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVSPLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATGIDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription[edit | edit source]

  • transcription start site:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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