⊟Summary[edit | edit source]
- organism: Streptococcus pneumoniae SPN034183
- locus tag: SPN034183_RS04725 [old locus tag: SPN034183_08660 ]
- pan locus tag?: PNEUPAN001964000
- symbol: pavA
- pan gene symbol?: pavA
- synonym:
- product: Rqc2 family fibronectin-binding protein PavA
⊟Additional information (user-provided)[edit | edit source]
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SPN034183_RS04725 [old locus tag: SPN034183_08660 ]
- symbol: pavA
- product: Rqc2 family fibronectin-binding protein PavA
- replicon: chromosome
- strand: -
- coordinates: 879103..880758
- length: 1656
- essential: unknown
⊟Accession numbers[edit | edit source]
- Location: NC_021028 (879103..880758) NCBI
- BioCyc:
- MicrobesOnline:
- PneumoBrowse for strain D39V: SPV_0854 PneumoBrowse
⊟Phenotype[edit | edit source]
⊟Additional information (user-provided)[edit | edit source]
⊟DNA sequence[edit | edit source]
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1621ATGTCATTTGACGGATTTTTTTTACACCACATAGTTGAGGAATTGCGAAGCGAATTAGTG
AATGGTCGCATCCAGAAAATCAATCAGCCTTTTGAACAAGAGTTGGTCTTGCAAATCCGC
AGCAATCGCCAAAGCCATCGCCTGCTCCTTTCTGCCCATCCAGTTTTTGGACGCATTCAG
CTGACCCAAACGACTTTTGAAAATCCAGCCCAACCTTCTACCTTTATCATGGTTTTGAGA
AAGTATTTGCAGGGGGCCCTGATTGAGTCGATTGAGCAAGTGGAAAATGACCGTATTGTG
GAAATTACAGTTTCCAATAAAAACGAGATTGGAGACCATATCCAGGCTACCTTGATTATC
GAAATTATGGGGAAACACAGTAATATTCTACTGGTCGATAAAAGCAGTCATAAAATCCTC
GAAGTTATCAAACACGTCGGCTTTTCACAAAATAGCTACCGCACCTTACTTCCAGGATCG
ACCTATATCGCTCCGCCAAGTACAGAATCTCTCAATCCTTTTACTATCAAGGATGAAAAG
CTCTTTGAAATCCTGCAAACCCAAGAACTAACAGCAAAAAATCTTCAAAGCCTCTTTCAA
GGTCTGGGACGCGATACGGCAAATGAATTGGAAAGGATACTGGTTAGTGAAAAACTTTCC
GCTTTCCGAAATTTTTTCAATCAGGAAACCAAGCCATGCTTGACTGAGACTTCCTTCAGT
CCAGTTCCTTTTGCAAATCAGGTGGGAGAGCCTTTTGCAAATCTTTCTGATTTGTTGGAC
ACCTACTATAAGGATAAGGCTGAGCGCGACCGCGTCAAACAGCAGGCCAGTGAACTGATT
CGTCGTGTTGAAAATGAACTTCAGAAAAACCGACACAAACTCAAAAAACAGGAAAAAGAG
TTACTGGCGACAGACAACGCTGAAGAATTTCGTCAAAAAGGAGAATTGCTGACAACCTTC
CTCCACCAAGTGCCTAACGACCAAGACCAGGTTATCCTAGACAACTACTATACCAACCAA
CCTATCATGATTGCGCTTGATAAGGCTCTGACTCCCAACCAGAATGCCCAACGCTATTTT
AAACGGTATCAGAAACTCAAAGAAGCTGTCAAATACTTGACTGATTTGATTGAAGAAACC
AAAGCCACTATTCTCTATCTGGAAAGTGTAGAAACCGTCCTCAACCAAGCTGGACTGGAA
GAAATCGCTGAAATCCGTGAAGAATTGATTCAAACAGGTTTTATCCGCAGAAGACAACGG
GAGAAAATCCAGAAACGCAAAAAACTAGAACAATATCTAGCAAGCGATGGCAAAACCATC
ATCTATGTCGGACGAAACAATCTTCAAAATGAGGAATTGACCTTTAAAATGGCCCGCAAG
GAGGAACTTTGGTTCCATGCTAAGGACATTCCTGGAAGCCATGTTGTCATCTCAGGAAAT
CTTGACCCATCTGATGCAGTCAAGACAGACGCAGCAGAGTTAGCTGCCTACTTCTCTCAA
GGGCGCCTGTCGAATCTGGTGCAGGTAGATATGATTGAAGTCAAAAAACTCAATAAACCA
ACTGGTGGAAAACCCGGCTTTGTCACTTACACAGGACAAAAGACCCTCCGCGTCACACCA
GACTCCAAAAAAATTGCATCCATGAAAAAATCCTGA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SPN034183_RS04725 [old locus tag: SPN034183_08660 ]
- symbol: PavA
- description: Rqc2 family fibronectin-binding protein PavA
- length: 551
- theoretical pI: 8.69187
- theoretical MW: 63290.8
- GRAVY: -0.517241
⊟Function[edit | edit source]
- TIGRFAM:
- TheSEED: see SPN034183_08660
- PFAM: H2TH (CL0303) NFACT_N; NFACT N-terminal and middle domains (PF05833; HMM-score: 497.2)and 6 moreNFACT_RNA-bind (CL0684) NFACT-R_1; NFACT protein RNA binding domain (PF05670; HMM-score: 32.8)NFACT-R_2; NFACT protein RNA binding domain (PF18297; HMM-score: 30.8)no clan defined RbcX; RbcX protein (PF02341; HMM-score: 13.1)Siah-Interact_N; Siah interacting protein, N terminal (PF09032; HMM-score: 11.4)DUF5798; Family of unknown function (DUF5798) (PF19111; HMM-score: 10.7)ALC; Phage ALC protein (PF17527; HMM-score: 6.9)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- DeepLocPro: Cytoplasmic
- Cytoplasmic Score: 0.7323
- Cytoplasmic Membrane Score: 0.0026
- Cell wall & surface Score: 0.0993
- Extracellular Score: 0.1658
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.002942
- TAT(Tat/SPI): 0.001268
- LIPO(Sec/SPII): 0.000387
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI:
- RefSeq: WP_000006695 NCBI
- UniProt:
⊟Additional information (user-provided)[edit | edit source]
⊟Protein sequence[edit | edit source]
- MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQSHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEITVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGSTYIAPPSTESLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEKLSAFRNFFNQETKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQASELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILDNYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVETVLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQNEELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQVDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS
⊟Experimental data[edit | edit source]
- protein localization:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Additional information (user-provided)[edit | edit source]
⊟Transcription[edit | edit source]
- transcription start site:
⊟Expression data[edit | edit source]
- PneumoExpress for strain D39V: SPV_0854 PneumoExpress
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟'lacZ' fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Additional information (user-provided)[edit | edit source]
⊟Other information (user-provided)[edit | edit source]
You can add further information about the gene and protein here. [edit]
⊟Literature[edit | edit source]
⊟References[edit | edit source]
⊟Relevant publications[edit | edit source]
A R Holmes, R McNab, K W Millsap, M Rohde, S Hammerschmidt, J L Mawdsley, H F Jenkinson
The pavA gene of Streptococcus pneumoniae encodes a fibronectin-binding protein that is essential for virulence.
Mol Microbiol: 2001, 41(6);1395-408
[PubMed:11580843] [WorldCat.org] [DOI] (P p)Daniela Pracht, Christine Elm, Joachim Gerber, Simone Bergmann, Manfred Rohde, Marleen Seiler, Kwang S Kim, Howard F Jenkinson, Roland Nau, Sven Hammerschmidt
PavA of Streptococcus pneumoniae modulates adherence, invasion, and meningeal inflammation.
Infect Immun: 2005, 73(5);2680-9
[PubMed:15845469] [WorldCat.org] [DOI] (P p)Nadja Noske, Ulrike Kämmerer, Manfred Rohde, Sven Hammerschmidt
Pneumococcal interaction with human dendritic cells: phagocytosis, survival, and induced adaptive immune response are manipulated by PavA.
J Immunol: 2009, 183(3);1952-63
[PubMed:19570831] [WorldCat.org] [DOI] (I p)A Kadioglu, H Brewin, T Härtel, J L Brittan, M Klein, S Hammerschmidt, H F Jenkinson
Pneumococcal protein PavA is important for nasopharyngeal carriage and development of sepsis.
Mol Oral Microbiol: 2010, 25(1);50-60
[PubMed:20331793] [WorldCat.org] [DOI] (I p)Sajida Kanwal, Inga Jensch, Gottfried J Palm, Mark Brönstrup, Manfred Rohde, Thomas P Kohler, Daniela Somplatzki, Werner Tegge, Howard F Jenkinson, Sven Hammerschmidt
Mapping the recognition domains of pneumococcal fibronectin-binding proteins PavA and PavB demonstrates a common pattern of molecular interactions with fibronectin type III repeats.
Mol Microbiol: 2017, 105(6);839-859
[PubMed:28657670] [WorldCat.org] [DOI] (I p)Kartik Manne, Sthanam V L Narayana, Debasish Chattopadhyay
Crystal structure of the N-terminal domain of the fibronectin-binding protein PavA from Streptococcus pneumoniae.
Acta Crystallogr F Struct Biol Commun: 2019, 75(Pt 10);657-662
[PubMed:31584015] [WorldCat.org] [DOI] (I p)