PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BM6001

serotype 19F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7465

serotype 1

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 05-APR-2025

⊟Summary

• organism: Streptococcus pneumoniae SPN994039
• locus tag: SPN994039_RS08305 [old locus tag: SPN994039_15370 ]
• pan locus tag^?: PNEUPAN003250000
• symbol: queF
• pan gene symbol^?: queF
• synonym:
• product: preQ(1) synthase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SPN994039_RS08305 [old locus tag: SPN994039_15370 ]
• symbol: queF
• product: preQ(1) synthase
• replicon: chromosome
• strand: +
• coordinates: 1563836..1564369
• length: 534
• essential: unknown other strains

⊟Accession numbers

• Location: NC_021005 (1563836..1564369) NCBI
• BioCyc:
• MicrobesOnline:
• PneumoBrowse for strain D39V: SPV_1568 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  
  ATGAAACGTCAATCAGCCTTGGTCGTCTTTAGTGGTGGTCAAGATTCAACAACCTGCCTT
  TTCTGGGCCATGCAACACTATGAAACAGTCGAAGCTGTCACCTTTGCCTACGGCCAACGT
  CATCACCTCGAAATTCAAATTACTAGAGAAATCGCTAAGGAACAGGGCATTCGTCACCAT
  ATCCTCGATATGTCTCTGCTGGGACAAATCACTGCTCAGCCAGACTTTGCGACGATTCAT
  ATTTCCTACATTCCTGACAAGCTCTGTGTCGAGTCAAAATCCCTCAAACTATATCTATTT
  AGCTACCGAAACCACGGAGATTTCCACGAAAACTGTATCAACACCATCGGGAAAGACTTG
  GTCAACTTGCTAGACCCTCGCTATTTAGAAGTCTGGGGAAAATTCACTCCGCGCGGTGGC
  ATTTCAATCGACCCCTACTATAACTACGGTAAGCAAGGAACTAAGTATGAGGGCTTGGCA
  GAACAACGCCTCTTCCAACACGACCTTTATCCAGAGAAAATTGACAACCGCTAA
  60
  120
  180
  240
  300
  360
  420
  480
  534
  

⊟Protein

⊟General

• locus tag: SPN994039_RS08305 [old locus tag: SPN994039_15370 ]
• symbol: QueF
• description: preQ(1) synthase
• length: 177
• theoretical pI: 7.01406
• theoretical MW: 20542.2
• GRAVY: -0.448588

⊟Function

• reaction:
  EC 1.7.1.13?  ExPASy
  PreQ¹ synthase 7-aminomethyl-7-carbaguanine + 2 NADP⁺ = 7-cyano-7-carbaguanine + 2 NADPH
• TIGRFAM:
  7-cyano-7-deazaguanine reductase (TIGR03139; EC 1.7.1.13; HMM-score: 128.6)
  and 11 more
  Unknown function General queuosine biosynthesis protein QueC (TIGR00364; HMM-score: 86.5)
  Genetic information processing Transcription RNA processing queuine synthase (TIGR03138; EC 1.7.1.13; HMM-score: 72.3)
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA(Ile)-lysidine synthetase (TIGR02432; EC 6.3.4.-; HMM-score: 18.3)
  Hypothetical proteins Conserved TIGR00268 family protein (TIGR00268; HMM-score: 18.1)
  Metabolism Amino acid biosynthesis Aspartate family asparagine synthase (glutamine-hydrolyzing) (TIGR01536; EC 6.3.5.4; HMM-score: 17.1)
  MJ0570-related uncharacterized domain (TIGR00290; HMM-score: 15.7)
  Metabolism Amino acid biosynthesis Glutamate family argininosuccinate synthase (TIGR00032; EC 6.3.4.5; HMM-score: 15.6)
  arCOG00187 universal archaeal metal-binding-domain/4Fe-4S-binding-domain containing ABC transporter, ATP-binding protein (TIGR03679; HMM-score: 13.2)
  Hypothetical proteins Conserved TIGR00289 family protein (TIGR00289; HMM-score: 12.7)
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA sulfurtransferase ThiI (TIGR00342; EC 2.8.1.4; HMM-score: 12.3)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine tRNA sulfurtransferase ThiI (TIGR00342; EC 2.8.1.4; HMM-score: 12.3)
• TheSEED: data available for Hungary19A-6, TIGR4
• PFAM:
  THBO-biosyn (CL0334) QueF; QueF-like protein (PF14489; HMM-score: 117.2)
  and 7 more
  HUP (CL0039) QueC; Queuosine biosynthesis protein QueC (PF06508; HMM-score: 83.2)
  Asn_synthase; Asparagine synthase (PF00733; HMM-score: 19.7)
  THBO-biosyn (CL0334) QueF_N; Nitrile reductase, 7-cyano-7-deazaguanine-reductase N-term (PF14819; HMM-score: 18.7)
  HUP (CL0039) ATP_bind_3; PP-loop family (PF01171; HMM-score: 17.7)
  Diphthami_syn_2; Diphthamide synthase (PF01902; HMM-score: 16.8)
  Arginosuc_synth; Arginosuccinate synthase (PF00764; HMM-score: 13)
  NAD_synthase; NAD synthase (PF02540; HMM-score: 10.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.025312
  • TAT(Tat/SPI): 0.000773
  • LIPO(Sec/SPII): 0.018926
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: WP_000828787 NCBI
• UniProt:

⊟Protein sequence

• MKRQSALVVFSGGQDSTTCLFWAMQHYETVEAVTFAYGQRHHLEIQITREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSYRNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLAEQRLFQHDLYPEKIDNR

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Expression data

• PneumoExpress for strain D39V: SPV_1568 PneumoExpress

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications