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PangenomeTIGR4
serotype 4
D39serotype 2
D39Vserotype 2
Hungary19A-6serotype 19A
EF3030serotype 19F
670-6Bserotype 6B
6A-10serotype 6A
70585serotype 5
A026serotype 19F
A66serotype 3
AP200serotype 11A
ASP0581serotype 12F
ATCC 49619serotype 19F
ATCC 700669serotype 23F
BM6001serotype 19F
BVJ1JLserotype 1
CGSP14serotype 14
G54serotype 19F
HU-OHserotype 3
Hu15serotype 19A
Hu17serotype 19A
INV104serotype 1
INV200serotype 14
JJAserotype 14
MDRSPN001serotype 19F
NCTC7465serotype 1
NCTC7466serotype 2
NU83127serotype 4
OXC141serotype 3
P1031serotype 1
R6serotype 2
SP49serotype 19A
SPN032672serotype 1
SPN034156serotype 3
SPN034183serotype 3
SPN994038serotype 3
SPN994039serotype 3
SPNA45serotype 3
ST556serotype 19F
TCH8431/19Aserotype 19A
Taiwan19F-14serotype 19F
Xen35serotype 4
gamPNI0373serotype 1
NCBI: 25-NOV-2024
⊟Summary[edit | edit source]
- organism: Streptococcus pneumoniae R6
- locus tag: SPR_RS07950 [old locus tag: spr1603 ]
- pan locus tag?: PNEUPAN003250000
- symbol: queF
- pan gene symbol?: queF
- synonym:
- product: preQ(1) synthase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
⊟Accession numbers[edit | edit source]
- Location: NC_003098 (1578836..1579369) NCBI
- BioCyc: see spr1603
- MicrobesOnline: see spr1603
- PneumoBrowse for strain D39V: SPV_1568 PneumoBrowse
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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481ATGAAACATCAATCAGCCTTGGTCGTCTTTAGTGGCGGTCAAGATTCAACAACCTGCCTT
TTCTGGACCATGCAACACTATGAAACAGTCGAAGCTGTCACCTTTGCCTACGGCCAACAT
CATCACCTCGAAATTCAAATTACTAGAGAAATCGCTAAGGAACAGGGCATTCGTCACCAT
ATCCTCGATATGTCTCTGCTGGGACAAATCACTGCTCAGCCAGACTTTGCGACGATTCAT
ATTTCCTACATTCCTGACAAGCTCTGTGTCGAGTCAAAATCCCTCAAACTATATCTATTT
AGCTACCGAAACCACGGAGATTTCCACGAAAACTGTATCAACACCATCGGGAAAGACTTG
GTCAACTTGCTAGACCCTCGCTATTTAGAAGTCTGGGGAAAATTCACTCCGCGCGGTGGC
ATTTCAATCGACCCCTACTACAACTACGGTAAGCAAGGAACTAAGTATGAGGGCTTGGCA
GAACAACGCCTCTTCCAACACGACCTTTATCCAGAGAAAATTGACAACCGCTAA60
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534
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SPR_RS07950 [old locus tag: spr1603 ]
- symbol: QueF
- description: preQ(1) synthase
- length: 177
- theoretical pI: 6.73793
- theoretical MW: 20534.1
- GRAVY: -0.448023
⊟Function[edit | edit source]
- reaction: EC 1.7.1.13? ExPASyPreQ1 synthase 7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH
- TIGRFAM: 7-cyano-7-deazaguanine reductase (TIGR03139; EC 1.7.1.13; HMM-score: 128.5)and 11 moreUnknown function General queuosine biosynthesis protein QueC (TIGR00364; HMM-score: 82.1)Transcription RNA processing queuine synthase (TIGR03138; EC 1.7.1.13; HMM-score: 72.4)Hypothetical proteins Conserved TIGR00268 family protein (TIGR00268; HMM-score: 18)Protein synthesis tRNA and rRNA base modification tRNA(Ile)-lysidine synthetase (TIGR02432; EC 6.3.4.-; HMM-score: 17.4)Amino acid biosynthesis Aspartate family asparagine synthase (glutamine-hydrolyzing) (TIGR01536; EC 6.3.5.4; HMM-score: 16.7)Amino acid biosynthesis Glutamate family argininosuccinate synthase (TIGR00032; EC 6.3.4.5; HMM-score: 16)MJ0570-related uncharacterized domain (TIGR00290; HMM-score: 14.4)Protein synthesis tRNA and rRNA base modification tRNA sulfurtransferase ThiI (TIGR00342; EC 2.8.1.4; HMM-score: 11.8)Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine tRNA sulfurtransferase ThiI (TIGR00342; EC 2.8.1.4; HMM-score: 11.8)arCOG00187 universal archaeal metal-binding-domain/4Fe-4S-binding-domain containing ABC transporter, ATP-binding protein (TIGR03679; HMM-score: 11.8)Hypothetical proteins Conserved TIGR00289 family protein (TIGR00289; HMM-score: 11.5)
- TheSEED: see spr1603
- PFAM: THBO-biosyn (CL0334) QueF; QueF-like protein (PF14489; HMM-score: 116.8)and 6 moreHUP (CL0039) QueC; Queuosine biosynthesis protein QueC (PF06508; HMM-score: 78.5)THBO-biosyn (CL0334) QueF_N; Nitrile reductase, 7-cyano-7-deazaguanine-reductase N-term (PF14819; HMM-score: 18.5)HUP (CL0039) Asn_synthase; Asparagine synthase (PF00733; HMM-score: 17.2)ATP_bind_3; PP-loop family (PF01171; HMM-score: 17.1)Diphthami_syn_2; Diphthamide synthase (PF01902; HMM-score: 15.3)Arginosuc_synth; Arginosuccinate synthase (PF00764; HMM-score: 13.4)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.013537
- TAT(Tat/SPI): 0.000307
- LIPO(Sec/SPII): 0.008262
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MKHQSALVVFSGGQDSTTCLFWTMQHYETVEAVTFAYGQHHHLEIQITREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSYRNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLAEQRLFQHDLYPEKIDNR
⊟Experimental data[edit | edit source]
- protein localization:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Expression data[edit | edit source]
- PneumoExpress for strain D39V: SPV_1568 PneumoExpress
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Jane A Thanassi, Sandra L Hartman-Neumann, Thomas J Dougherty, Brian A Dougherty, Michael J Pucci
Identification of 113 conserved essential genes using a high-throughput gene disruption system in Streptococcus pneumoniae.
Nucleic Acids Res: 2002, 30(14);3152-62
[PubMed:12136097] [WorldCat.org] [DOI] (I p)