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serotype 4
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D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
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serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
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CGSP14
serotype 14
G54
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HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 30-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae R6
  • locus tag: spr1603 [new locus tag: SPR_RS07950 ]
  • pan locus tag?: PNEUPAN003250000
  • symbol: spr1603
  • pan gene symbol?: queF
  • synonym:
  • product: Conserved hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: spr1603 [new locus tag: SPR_RS07950 ]
  • symbol: spr1603
  • product: Conserved hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1578797..1579369
  • length: 573
  • essential: yes [1] DEG

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    TTGCGTGCTCTGAACATTTCCCATTTTGGAAGGAGTACTATGAAACATCAATCAGCCTTG
    GTCGTCTTTAGTGGCGGTCAAGATTCAACAACCTGCCTTTTCTGGACCATGCAACACTAT
    GAAACAGTCGAAGCTGTCACCTTTGCCTACGGCCAACATCATCACCTCGAAATTCAAATT
    ACTAGAGAAATCGCTAAGGAACAGGGCATTCGTCACCATATCCTCGATATGTCTCTGCTG
    GGACAAATCACTGCTCAGCCAGACTTTGCGACGATTCATATTTCCTACATTCCTGACAAG
    CTCTGTGTCGAGTCAAAATCCCTCAAACTATATCTATTTAGCTACCGAAACCACGGAGAT
    TTCCACGAAAACTGTATCAACACCATCGGGAAAGACTTGGTCAACTTGCTAGACCCTCGC
    TATTTAGAAGTCTGGGGAAAATTCACTCCGCGCGGTGGCATTTCAATCGACCCCTACTAC
    AACTACGGTAAGCAAGGAACTAAGTATGAGGGCTTGGCAGAACAACGCCTCTTCCAACAC
    GACCTTTATCCAGAGAAAATTGACAACCGCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    573

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: spr1603 [new locus tag: SPR_RS07950 ]
  • symbol: spr1603
  • description: Conserved hypothetical protein
  • length: 190
  • theoretical pI: 7.15738
  • theoretical MW: 22005.8
  • GRAVY: -0.436316

⊟Function[edit | edit source]

  • TIGRFAM:
    7-cyano-7-deazaguanine reductase (TIGR03139; EC 1.7.1.13; HMM-score: 128.3)
    and 11 more
    Unknown function General queuosine biosynthesis protein QueC (TIGR00364; HMM-score: 81.9)
    Genetic information processing Transcription RNA processing queuine synthase (TIGR03138; EC 1.7.1.13; HMM-score: 72.2)
    Hypothetical proteins Conserved TIGR00268 family protein (TIGR00268; HMM-score: 18.6)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA(Ile)-lysidine synthetase (TIGR02432; EC 6.3.4.-; HMM-score: 17.2)
    Metabolism Amino acid biosynthesis Aspartate family asparagine synthase (glutamine-hydrolyzing) (TIGR01536; EC 6.3.5.4; HMM-score: 16.9)
    Metabolism Amino acid biosynthesis Glutamate family argininosuccinate synthase (TIGR00032; EC 6.3.4.5; HMM-score: 15.8)
    MJ0570-related uncharacterized domain (TIGR00290; HMM-score: 14.2)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA sulfurtransferase ThiI (TIGR00342; EC 2.8.1.4; HMM-score: 11.9)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine tRNA sulfurtransferase ThiI (TIGR00342; EC 2.8.1.4; HMM-score: 11.9)
    arCOG00187 universal archaeal metal-binding-domain/4Fe-4S-binding-domain containing ABC transporter, ATP-binding protein (TIGR03679; HMM-score: 11.6)
    Hypothetical proteins Conserved TIGR00289 family protein (TIGR00289; HMM-score: 11.3)
  • TheSEED  :
    • NADPH-dependent 7-cyano-7-deazaguanine reductase (EC 1.7.1.13)
    RNA Metabolism RNA processing and modification Queuosine-Archaeosine Biosynthesis  NADPH dependent preQ0 reductase (EC 1.7.1.13)
  • PFAM:
    THBO-biosyn (CL0334) QueF; QueF-like protein (PF14489; HMM-score: 116.6)
    and 7 more
    HUP (CL0039) QueC; Queuosine biosynthesis protein QueC (PF06508; HMM-score: 78.3)
    THBO-biosyn (CL0334) QueF_N; Nitrile reductase, 7-cyano-7-deazaguanine-reductase N-term (PF14819; HMM-score: 18.4)
    HUP (CL0039) Asn_synthase; Asparagine synthase (PF00733; HMM-score: 18)
    ATP_bind_3; PP-loop family (PF01171; HMM-score: 16.9)
    Diphthami_syn_2; Diphthamide synthase (PF01902; HMM-score: 15.1)
    Arginosuc_synth; Arginosuccinate synthase (PF00764; HMM-score: 13.2)
    NAD_synthase; NAD synthase (PF02540; HMM-score: 11.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.023607
    • TAT(Tat/SPI): 0.000633
    • LIPO(Sec/SPII): 0.013445
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRALNISHFGRSTMKHQSALVVFSGGQDSTTCLFWTMQHYETVEAVTFAYGQHHHLEIQITREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSYRNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLAEQRLFQHDLYPEKIDNR

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Jane A Thanassi, Sandra L Hartman-Neumann, Thomas J Dougherty, Brian A Dougherty, Michael J Pucci
    Identification of 113 conserved essential genes using a high-throughput gene disruption system in Streptococcus pneumoniae.
    Nucleic Acids Res: 2002, 30(14);3152-62
    [PubMed:12136097] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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