PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 02-APR-2021

⊟Summary

• organism: Streptococcus pneumoniae Hu15
• locus tag: SPNHU15_RS09290 [old locus tag: SPNHU15_01965 ]
• pan locus tag^?: PNEUPAN003517000
• symbol: tsaE
• pan gene symbol^?: tsaE
• synonym:
• product: tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SPNHU15_RS09290 [old locus tag: SPNHU15_01965 ]
• symbol: tsaE
• product: tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE
• replicon: chromosome
• strand: -
• coordinates: 1843490..1843933
• length: 444
• essential: unknown

⊟Accession numbers

• Location: NZ_CP020551 (1843490..1843933) NCBI
• BioCyc:
• MicrobesOnline:
• PneumoBrowse for strain D39V: SPV_1743 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  
  ATGTACACAAAAAATGAAGAAGAGTTGCAAGCCTTAGGGGAGCGTTTGGGCCATCTATTA
  GCAAAGAATGATGTTTTAATCTTAATTGGAGAACTGGGTGCAGGTAAAACGACCTTTACT
  AAAGGACTTGCAAAAGGATTACAGATTTCTCAAATGATTAAAAGTCCTACCTATACTATC
  GTGAGAGAGTATGAAGGTCGACTTCCACTTTATCACCTAGATGTTTATCGTATTGAAGGA
  GATGCTGATTCTATCGACTTGGATGAGTTTATCTTTGGTGGCGGCGTGACTGTTATTGAG
  TGGGGAAATCTCTTAGGAGATGCCTTGCCAGATGCTTATTTAGAATTGGAAATTCTAAAA
  GAAGCAGATGGACGCCGTTTAAATTTTCAGGCAAAGGGTTTGCGTGCTGAGAAATTGTTA
  GAGGAGCTTCAATATGGAGTATGA
  60
  120
  180
  240
  300
  360
  420
  444
  

⊟Protein

⊟General

• locus tag: SPNHU15_RS09290 [old locus tag: SPNHU15_01965 ]
• symbol: TsaE
• description: tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE
• length: 147
• theoretical pI: 4.45213
• theoretical MW: 16413.7
• GRAVY: -0.154422

⊟Function

• TIGRFAM:
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA threonylcarbamoyl adenosine modification protein YjeE (TIGR00150; HMM-score: 112.7)
  and 6 more
  Genetic information processing Protein fate Protein and peptide secretion and trafficking putative secretion ATPase, PEP-CTERM locus subfamily (TIGR03015; HMM-score: 14.2)
  KaiC domain protein, AF_0351 family (TIGR03880; HMM-score: 12.8)
  Genetic information processing Protein synthesis tRNA aminoacylation L-seryl-tRNA(Sec) kinase (TIGR03574; EC 2.7.1.164; HMM-score: 11.9)
  Metabolism Central intermediary metabolism Phosphorus compounds phosphonate C-P lyase system protein PhnK (TIGR02323; HMM-score: 11.6)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking signal recognition particle protein (TIGR00959; HMM-score: 10.3)
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides ATP-dependent Clp protease ATP-binding subunit ClpA (TIGR02639; HMM-score: 10.3)
• TheSEED: data available for D39, Hungary19A-6, TIGR4
• PFAM:
  P-loop_NTPase (CL0023) TsaE; Threonylcarbamoyl adenosine biosynthesis protein TsaE (PF02367; HMM-score: 124.4)
  and 14 more
  AAA_33; AAA domain (PF13671; HMM-score: 17.7)
  NACHT; NACHT domain (PF05729; HMM-score: 15.2)
  AAA_22; AAA domain (PF13401; HMM-score: 15.1)
  AAA_18; AAA domain (PF13238; HMM-score: 14.1)
  ABC_tran; ABC transporter (PF00005; HMM-score: 13.5)
  RNA_helicase; RNA helicase (PF00910; HMM-score: 13.3)
  AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 12.7)
  PduV-EutP; Ethanolamine utilisation - propanediol utilisation (PF10662; HMM-score: 12.5)
  dNK; Deoxynucleoside kinase (PF01712; HMM-score: 12.4)
  AAA_30; AAA domain (PF13604; HMM-score: 12.3)
  RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 12.2)
  AAA_25; AAA domain (PF13481; HMM-score: 12.2)
  AAA_7; P-loop containing dynein motor region (PF12775; HMM-score: 11.6)
  Ploopntkinase3; P-loop Nucleotide Kinase3 (PF18751; HMM-score: 11.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.008437
  • TAT(Tat/SPI): 0.009704
  • LIPO(Sec/SPII): 0.001262
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: WP_000288230 NCBI
• UniProt:

⊟Protein sequence

• MYTKNEEELQALGERLGHLLAKNDVLILIGELGAGKTTFTKGLAKGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWGNLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for D39, TIGR4

⊟Expression data

• PneumoExpress for strain D39V: SPV_1743 PneumoExpress

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications