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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 02-MAY-2018

Summary[edit | edit source]

  • organism: Streptococcus pneumoniae D39
  • locus tag: SPD_1743 [new locus tag: SPD_RS09285 ]
  • pan locus tag?: PNEUPAN003517000
  • symbol: SPD_1743
  • pan gene symbol?: tsaE
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SPD_1743 [new locus tag: SPD_RS09285 ]
  • symbol: SPD_1743
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1736705..1737148
  • length: 444
  • essential: unknown

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGTACACAAAAAATGAAGAAGAGTTGCAAGCCTTAGGGGAGCGTTTGGGCCATCTATTA
    GCAAAGAATGATGTTTTAATCTTAACTGGAGAACTGGGTGCAGGTAAAACGACCTTTACT
    AAAGGACTTGCAAAAGGATTACAGATTTCTCAAATGATTAAAAGTCCCACCTATACTATC
    GTGAGAGAGTATGAAGGTCGACTTCCACTTTATCACCTAGATGTTTATCGTATTGAAGGA
    GATGCTGATTCTATCGACTTGGATGAGTTTATCTTTGGTGGCGGCGTGACTGTTATTGAG
    TGGGGAAATCTCTTAGGAGATGCCTTGCCAGATGCTTATTTAGAATTGGAAATTCTAAAA
    GAAGCAGATGGACGCCGTTTAAATTTTCAGGCAAAGGGTTTGCGTGCTGAGAAATTGTTA
    GAGGAGCTTCAATATGGAGTATGA
    60
    120
    180
    240
    300
    360
    420
    444

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SPD_1743 [new locus tag: SPD_RS09285 ]
  • symbol: SPD_1743
  • description: hypothetical protein
  • length: 147
  • theoretical pI: 4.45213
  • theoretical MW: 16401.6
  • GRAVY: -0.189796

Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA threonylcarbamoyl adenosine modification protein YjeE (TIGR00150; HMM-score: 114.2)
    and 5 more
    Genetic information processing Protein fate Protein and peptide secretion and trafficking putative secretion ATPase, PEP-CTERM locus subfamily (TIGR03015; HMM-score: 17.3)
    Genetic information processing Protein synthesis tRNA aminoacylation L-seryl-tRNA(Sec) kinase (TIGR03574; EC 2.7.1.164; HMM-score: 15.4)
    Cellular processes Cellular processes Cell division cell division ATP-binding protein FtsE (TIGR02673; HMM-score: 12.1)
    Unknown function Enzymes of unknown specificity helicase, RecD/TraA family (TIGR01448; HMM-score: 10.7)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair checkpoint protein rad24 (TIGR00602; HMM-score: 9.7)
  • TheSEED  :
    • tRNA threonylcarbamoyladenosine biosynthesis protein TsaE
    Cell Wall and Capsule Cell Wall and Capsule - no subcategory YjeE  TsaE protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA
    and 2 more
    Regulation and Cell signaling Regulation and Cell signaling - no subcategory Cell envelope-associated LytR-CpsA-Psr transcriptional attenuators  TsaE protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA
    RNA Metabolism RNA processing and modification YrdC-YciO-Sua5 and associated protein families  TsaE protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA
  • PFAM:
    P-loop_NTPase (CL0023) TsaE; Threonylcarbamoyl adenosine biosynthesis protein TsaE (PF02367; HMM-score: 126)
    and 16 more
    AAA_18; AAA domain (PF13238; HMM-score: 16.1)
    AAA_33; AAA domain (PF13671; HMM-score: 16)
    NACHT; NACHT domain (PF05729; HMM-score: 15.6)
    AAA_22; AAA domain (PF13401; HMM-score: 15.3)
    AAA_30; AAA domain (PF13604; HMM-score: 13.9)
    RNA_helicase; RNA helicase (PF00910; HMM-score: 13.8)
    dNK; Deoxynucleoside kinase (PF01712; HMM-score: 13.5)
    AAA_25; AAA domain (PF13481; HMM-score: 13.1)
    NTPase_1; NTPase (PF03266; HMM-score: 12.9)
    AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 12.4)
    Rad17; Rad17 P-loop domain (PF03215; HMM-score: 12.3)
    cobW; CobW/HypB/UreG, nucleotide-binding domain (PF02492; HMM-score: 12.2)
    AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 12)
    AAA_7; P-loop containing dynein motor region (PF12775; HMM-score: 11.5)
    VirE; Virulence-associated protein E (PF05272; HMM-score: 11.3)
    Myosin_head; Myosin head (motor domain) (PF00063; HMM-score: 9.6)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.016718
    • TAT(Tat/SPI): 0.008564
    • LIPO(Sec/SPII): 0.001507
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLAKGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWGNLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV

Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator: ComE (activation) regulon
    ComE(response regulator)activating expression of early competence genes [1]

Expression data[edit | edit source]

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Jelle Slager, Rieza Aprianto, Jan-Willem Veening
    Refining the Pneumococcal Competence Regulon by RNA Sequencing.
    J Bacteriol: 2019, 201(13);
    [PubMed:30885934] [WorldCat.org] [DOI] (I e)

Relevant publications[edit | edit source]