From PneumoWiki
Jump to navigation Jump to search
PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 31-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae Taiwan19F-14
  • locus tag: SPT_0165 [new locus tag: SPT_RS00840 ]
  • pan locus tag?: PNEUPAN000794000
  • symbol: trmU
  • pan gene symbol?: mnmA
  • synonym:
  • product: tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPT_0165 [new locus tag: SPT_RS00840 ]
  • symbol: trmU
  • product: tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 147806..148927
  • length: 1122
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAGTGATAACTCTAAAACACGTGTTGTCGTGGGGATGAGTGGTGGTGTTGATTCGTCG
    GTGACGGCTCTTTTGCTCAAGGAGCAGGGCTACGATGTGATCGGTATCTTCATGAAGAAC
    TGGGATGACACAGATGAAAACGGCGTCTGTACGGCGACCGAAGATTACAAGGATGTGGTT
    GCGGTGGCAGACCAGATTGGCATTCCCTACTACTCTGTCAATTTTGAAAAAGAGTACTGG
    GACCGCGTTTTTGAGTATTTCCTAGCGGAATACCGTGCAGGGCGCACGCCAAATCCGGAC
    GTTATGTGCAACAAGGAAATCAAGTTCAAGGCCTTTTTGGACTATGCCATAACCTTGGGG
    GCAGACTATGTAGCGACTGGGCATTATGCTCGAGTGGCGCGTGATGAGGATGGTACCGTT
    CACATGCTTCGTGGCGTGGACAATGGCAAGGATCAGACCTATTTCCTCAGCCAACTTTCG
    CAAGAACAACTTCAAAAAACCATGTTCCCACTAGGACATTTGGAAAAGCCTGAAGTACGC
    AGACTAGCAGAAGAAGCAGGCCTTTCGACTGCTAAGAAGAAAGACTCGACAGGGATTTGC
    TTTATCGGAGAAAAGAACTTTAAAAACTTTCTCAGCAACTACCTGCCAGCTCAGCCTGGT
    CGCATGATGACTGTGGATGGTCGCGATATGGGCGAGCATGCAGGTCTTATGTACTATACA
    ATCGGTCAGCGTGGCGGACTCGGTATCGGTGGGCAACACGGCGGTGACAATGCCCCTTGG
    TTCGTTGTCGGAAAAGATCTAAGCAAGAATATTCTCTATGTAGGACAAGGATTCTACCAT
    GATTCGCTCATGTCAACTAGCCTAGAAGCCAGTCAAGTCCACTTTACTCGTGAAATGCCA
    GAAGAGTTTACGCTAGAATGTACGGCTAAATTCCGTTACCGTCAGCCTGACTCTAAGGTG
    ACCGTTCATGTCAAAGGAGAAAAGACAGAGGTCATCTTTGCGGAACCACAACGCGCGATT
    ACACCAGGACAGGCAGTTGTCTTTTACGATGGCGAAGAGTGTCTAGGTGGCGGTTTGATT
    GACAATGCTTACCGCGATGGACAAGTTTGTCAGTACATTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1122

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPT_0165 [new locus tag: SPT_RS00840 ]
  • symbol: TrmU
  • description: tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase
  • length: 373
  • theoretical pI: 4.88918
  • theoretical MW: 41681.7
  • GRAVY: -0.404826

⊟Function[edit | edit source]

  • reaction:
    EC 2.1.1.61?  ExPASy
    tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase S-adenosyl-L-methionine + tRNA containing 5-aminomethyl-2-thiouridine = S-adenosyl-L-homocysteine + tRNA containing 5-methylaminomethyl-2-thiouridylate
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase (TIGR00420; EC 2.1.1.61; HMM-score: 455.9)
    and 10 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridine nucleotides NAD+ synthetase (TIGR00552; EC 6.3.1.5; HMM-score: 28.4)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis GMP synthase (glutamine-hydrolyzing), C-terminal domain (TIGR00884; EC 6.3.5.2; HMM-score: 21.9)
    Hypothetical proteins Conserved TIGR00268 family protein (TIGR00268; HMM-score: 20)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA(Ile)-lysidine synthetase (TIGR02432; EC 6.3.4.-; HMM-score: 18.5)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA sulfurtransferase ThiI (TIGR00342; EC 2.8.1.4; HMM-score: 16.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine tRNA sulfurtransferase ThiI (TIGR00342; EC 2.8.1.4; HMM-score: 16.8)
    Unknown function General queuosine biosynthesis protein QueC (TIGR00364; HMM-score: 16.8)
    Metabolism Amino acid biosynthesis Glutamate family argininosuccinate synthase (TIGR00032; EC 6.3.4.5; HMM-score: 16.1)
    Metabolism Amino acid biosynthesis Aspartate family asparagine synthase (glutamine-hydrolyzing) (TIGR01536; EC 6.3.5.4; HMM-score: 12.8)
    asparagine synthase family amidotransferase (TIGR03104; HMM-score: 10.4)
  • TheSEED  :
    • tRNA-specific 2-thiouridylase MnmA
  • PFAM:
    HUP (CL0039) tRNA_Me_trans; tRNA methyl transferase HUP domain (PF03054; HMM-score: 294.1)
    and 7 more
    no clan defined tRNA_Me_trans_C; Aminomethyltransferase beta-barrel domain (PF20258; HMM-score: 83.5)
    PRC-barrel (CL0350) tRNA_Me_trans_M; tRNA methyl transferase PRC-barrel domain (PF20259; HMM-score: 78.4)
    HUP (CL0039) NAD_synthase; NAD synthase (PF02540; HMM-score: 28.8)
    QueC; Queuosine biosynthesis protein QueC (PF06508; HMM-score: 19.1)
    ThiI; Thiamine biosynthesis protein (ThiI) (PF02568; HMM-score: 16.9)
    Asn_synthase; Asparagine synthase (PF00733; HMM-score: 16.7)
    ATP_bind_3; PP-loop family (PF01171; HMM-score: 14)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9931
    • Cytoplasmic Membrane Score: 0.001
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0059
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005453
    • TAT(Tat/SPI): 0.000437
    • LIPO(Sec/SPII): 0.001206
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDTDENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVMCNKEIKFKAFLDYAITLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLSQEQLQKTMFPLGHLEKPEVRRLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQPGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQGFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGEKTEVIFAEPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: RegR* (repression) regulon
    RegR*(TF)important in Hyaluronate utilization; RegPrecise    transcription unit transferred from TIGR4 data RegPrecise 

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://pneumowiki.med.uni-greifswald.de/index.php?title=SPT_0165&oldid=165007"