PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BM6001

serotype 19F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7465

serotype 1

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 31-JAN-2014

⊟Summary

• organism: Streptococcus pneumoniae Taiwan19F-14
• locus tag: SPT_0377 [new locus tag: SPT_RS01910 ]
• pan locus tag^?: PNEUPAN001082000
• symbol: SPT_0377
• pan gene symbol^?: regR
• synonym:
• product: sugar binding transcriptional regulator RegR

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SPT_0377 [new locus tag: SPT_RS01910 ]
• symbol: SPT_0377
• product: sugar binding transcriptional regulator RegR
• replicon: chromosome
• strand: +
• coordinates: 340582..341583
• length: 1002
• essential: unknown

⊟Accession numbers

• Location: CP000921 (340582..341583) NCBI
• BioCyc: see SPT_RS01910
• MicrobesOnline: 7474129 MicrobesOnline
• PneumoBrowse for strain D39V: SPV_0301 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  
  TTGGAGAAGAAACTGACCATAAAAGATATTGCAGAAATGGCTCAGACCTCGAAAACAACC
  GTGTCATTTTACCTAAACGGGAAATATGAAAAAATGTCCCAAGAGACACGTGAAAAGATT
  GAAAAAGTTATTCATGAAACAAATTACAAACCGAGCATTGTTGCGCGTAGCTTAAACTCC
  AAACGAACAAAATTAATTGGTGTTTTGATTGGTGATATTACCAACAGTTTCTCAACCCAA
  ATTGTTAAGGGAATTGAGGATATCGCCAGCCAGAATGGCTACCAGGTAATGATAGGAAAT
  AGTAACTACAGCCAAGAGAGTGAGGACAGGTATATTGAAAGCATGCTTCTCTTGGGAGTA
  GACGGCTTTATTATTCAGCCTACCTCTAATTTCCGAAAATATTCTCGTATCATCGATGAG
  AAAAAGAAGAAAATGGTCTTTTTTGATAGTCGGCTCTATGAACACCGGACTAGCTGGGTC
  AAAACCAATAACTATGATGCCGTTTATGATATGACCCAGTCCTGTATCGAAAAAGGTTAT
  GAGCATTTTCTCTTGATTACAGCGGATACGAGTCGTTTGAGTACTCGGATTGAGCGGGCA
  AGTGGTTTTGTGGATGCTTTAACAGATGCTAATATGCGCCACGCTAGGCTAACCATTGAA
  GATAAGCATACGAATTTGGAACAAATTAAGGAATTTTTACAAAAAGAAATCAATCCCGAT
  GAAAAAACTCTGGTATTTGTTCCTAACTGTTGGGCCCTACCTCCAGTCTTTACCGTTATC
  AAAGAGTTGAATTATAACTTGCCACAAGTTGGGTTGATTGGTTTTGACAATACGGAGTGG
  ACTTGCTTTTCTTCTCCAAGTGTTTCGACGTTGGTTCAGCCCGCCTTTGAAGAAGGACAA
  CAGGCTACAAAGATTTTGATTGACCAGATTGAAGGCCTCAATCAAGAAGAAAGGCAACAA
  GTCTTGGATTGTAGTGTGAGTTGGAAAGAGTCGACTTTCTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1002
  

⊟Protein

⊟General

• locus tag: SPT_0377 [new locus tag: SPT_RS01910 ]
• symbol: SPT_0377
• description: sugar binding transcriptional regulator RegR
• length: 333
• theoretical pI: 5.51829
• theoretical MW: 38300.2
• GRAVY: -0.455255

⊟Function

• TIGRFAM:
  Signal transduction Regulatory functions DNA interactions catabolite control protein A (TIGR01481; HMM-score: 147.9)
  Signal transduction Regulatory functions DNA interactions D-fructose-responsive transcription factor (TIGR02417; HMM-score: 145.9)
  and 2 more
  Signal transduction Regulatory functions DNA interactions trehalose operon repressor (TIGR02405; HMM-score: 105)
  Signal transduction Regulatory functions DNA interactions transcriptional repressor BetI (TIGR03384; HMM-score: 15.2)
• TheSEED  :
  • Transcriptional regulator RegR, rpressor of hyaluronate and KDG utilization
• PFAM:
  HTH (CL0123) LacI; Bacterial regulatory proteins, lacI family (PF00356; HMM-score: 62.2)
  Periplas_BP (CL0144) Peripla_BP_1; Periplasmic binding proteins and sugar binding domain of LacI family (PF00532; HMM-score: 60.6)
  Peripla_BP_4; Periplasmic binding protein domain (PF13407; HMM-score: 59.8)
  Peripla_BP_3; Periplasmic binding protein-like domain (PF13377; HMM-score: 55.7)
  and 6 more
  HTH (CL0123) HTH_3; Helix-turn-helix (PF01381; HMM-score: 21.9)
  HTH_19; Helix-turn-helix domain (PF12844; HMM-score: 20.5)
  HTH_40; Helix-turn-helix domain (PF14493; HMM-score: 13)
  Terminase_5; Putative ATPase subunit of terminase (gpP-like) (PF06056; HMM-score: 12.5)
  GerE; Bacterial regulatory proteins, luxR family (PF00196; HMM-score: 12)
  HTH_38; Helix-turn-helix domain (PF13936; HMM-score: 12)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effector: Hyaluronate oligosaccharide phosphate
• genes regulated by RegR*, TF important in Hyaluronate utilizationRegPrecise

  repression

  SPT_0027 > SPT_0029 > SPT_0030 > SPT_0031 > SPT_0033 > SPT_0034 > SPT_0035 > dinD* > SPT_0037 > ychF > pth > mfd > hslR* > divIC* > SPT_0043 > SPT_0044 > tilS* > hpt > ftsH* > comX1* > tRNA-Glu-3* > rrs1 > tRNA-Ala-1* > rrl1 > rrf_1 > tRNA-Asn-1* > comW* > purA > tadA* > srpB > dut* > SPT_0062 > radA > pca* > prsW* > prs1* > polA* > yccU* > araT* > recO > plsX > acpP_1* > IS1381_1* > blpK* > blpO_2* > comA* > comB* > purC > SPT_0083 > purL* > purF > purM > purN > vanZ* > purH > purD > purE > purK > SPT_0093 > purB > bgaC* > gadV* > gadW* > gadE* > gadF* > agaS* > galM* > catE* > csoR* > deoD_1* > trkH_1* > cabP* > SPT_0113 > SPT_0115 > pavB* > saeR* > hk08* > rpsD > SPT_0120 > SPT_0121 > ABC-MSP_1* > ABC-MSP_2* > ABC-SBP_1* > glpE_2* > SPT_0126 > SPT_0127 > SPT_0128 > SPT_0129 > SPT_0130 > pgaC* > aatA* > lytN* > scaA* > scaB* > scaC* > apbA* > argG* > rtgC* > lagD_2* > SPT_0152 > rtgL* > SPT_0154 > rtgP* > rtgQ* > rtgT* > rtgU* > rtgY1* > SPT_0160 > rtgZ1* > SPT_0162 > pspA* > SPT_0164 > trmU > SPT_0166 > gidA > tsaB* > rimI > tsaD* > SPT_0179 > wfgD* > ABC-NP_1* > SPT_0183 > SPT_0184 > SPT_0185 > ugd* > mutR1* > SPT_0189 > vpoB* > vpoC* > ygaZ* > SPT_0194 > gshT* > metQ* > dapE* > metN* > metP* > SPT_0201 > yesM* > yesN* > SPT_0204 > nrdI* > hexB* > ruvA > tag* > SPT_0218 > SPT_0225 > SPT_0226 > SPT_0227 > SPT_0228 > SPT_0229 > SPT_0230 > SPT_0231 > SPT_0232 > ps105* > SPT_0234 > SPT_0235 > SPT_0236 > SPT_0237 > uvrA > ypdF* > spxA2* > SPT_0241 > SPT_0242 > yqgF* > SPT_0244 > folC* > SPT_0246 > cls* > ccs4* > nrdD > SPT_0250 > SPT_0251 > SPT_0252 > nrdG > udk_1* > rpsJ > rplC > rplD > rplW > rplB > rpsS > rplV > rpsC > rplP > rpmC > rpsQ > rplN > rplX > rplE* > rpsN* > rpsH > rplF* > rplR > rpsE > rpmD > rplO > secY* > adk* > infA > rpmJ > rpsM* > rpsK > rpoA > rplQ > SPT_0284 > SPT_0285 > gpmB1* > deoR* > sorC_2* > PTS-EIIA* > PTS-EIIB_2* > PTS-EIIC_2* > pfl* > talC* > gldA* > leuS > SPT_0303 > ydaF_1* > SPT_0305 > ruvB > SPT_0307 > uppS > cdsA > rseP > proS > bglA* > glmS > luxA* > rpsL > rpsG > fusA > polC > relB1* > relE1* > polC_1* > pepS* > SPT_0324 > rsuA-1* > pepC* > pbuO* > SPT_0334 > folP > sulB* > folE > sulD* > rplM > rpsI > SPT_0352 > SPT_0353 > celA* > SPT_0355 > celB* > celR* > celC* > SPT_0359 > celD* > SPT_0361 > xylS* > tnpA* > PTS-EII_3* > ugl* > PTS-EII_4* > PTS-EII_5* > PTS-EII_6* > yajC-1* > SPT_0376 > regR*

  spnHL*

  eda* < kdgK* < rpiB* < gno*

  transcription units transferred from TIGR4 data RegPrecise

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9091
  • Cytoplasmic Membrane Score: 0.0789
  • Cell wall & surface Score: 0.0005
  • Extracellular Score: 0.0115
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.008042
  • TAT(Tat/SPI): 0.000515
  • LIPO(Sec/SPII): 0.002346
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: ACO23375 NCBI
• UniProt:

⊟Protein sequence

• MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVIHETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSTQIVKGIEDIASQNGYQVMIGNSNYSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSRLYEHRTSWVKTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHARLTIEDKHTNLEQIKEFLQKEINPDEKTLVFVPNCWALPPVFTVIKELNYNLPQVGLIGFDNTEWTCFSSPSVSTLVQPAFEEGQQATKILIDQIEGLNQEERQQVLDCSVSWKESTF

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SPT_0370 > SPT_0371 > SPT_0372 > SPT_0373 > SPT_0374 > SPT_0375 > SPT_0376 > SPT_0377 > SPT_0378

⊟Regulation

• regulator: RegR* (repression) regulon

  RegR*

  (TF)

  important in Hyaluronate utilization; RegPrecise    transcription unit transferred from TIGR4 data RegPrecise

⊟Expression data

• PneumoExpress for strain D39V: SPV_0301 PneumoExpress

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Sabine Chapuy-Regaud, A David Ogunniyi, Nicole Diallo, Yvette Huet, Jean-François Desnottes, James C Paton, Sonia Escaich, Marie-Claude Trombe
RegR, a global LacI/GalR family regulator, modulates virulence and competence in Streptococcus pneumoniae.
Infect Immun: 2003, 71(5);2615-25
[PubMed:12704136] [WorldCat.org] [DOI] (P p)