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PangenomeTIGR4
serotype 4
D39serotype 2
D39Vserotype 2
Hungary19A-6serotype 19A
EF3030serotype 19F
670-6Bserotype 6B
6A-10serotype 6A
70585serotype 5
A026serotype 19F
A66serotype 3
AP200serotype 11A
ASP0581serotype 12F
ATCC 49619serotype 19F
ATCC 700669serotype 23F
BM6001serotype 19F
BVJ1JLserotype 1
CGSP14serotype 14
G54serotype 19F
HU-OHserotype 3
Hu15serotype 19A
Hu17serotype 19A
INV104serotype 1
INV200serotype 14
JJAserotype 14
MDRSPN001serotype 19F
NCTC7465serotype 1
NCTC7466serotype 2
NU83127serotype 4
OXC141serotype 3
P1031serotype 1
R6serotype 2
SP49serotype 19A
SPN032672serotype 1
SPN034156serotype 3
SPN034183serotype 3
SPN994038serotype 3
SPN994039serotype 3
SPNA45serotype 3
ST556serotype 19F
TCH8431/19Aserotype 19A
Taiwan19F-14serotype 19F
Xen35serotype 4
gamPNI0373serotype 1
NCBI: 31-JAN-2014
⊟Summary[edit | edit source]
- organism: Streptococcus pneumoniae TIGR4
- locus tag: SP_0779 [new locus tag: SP_RS03800 ]
- pan locus tag?: PNEUPAN001721000
- symbol: trmD
- pan gene symbol?: trmD
- synonym:
- product: tRNA (guanine-N1)-methyltransferase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SP_0779 [new locus tag: SP_RS03800 ]
- symbol: trmD
- product: tRNA (guanine-N1)-methyltransferase
- replicon: chromosome
- strand: +
- coordinates: 734233..734952
- length: 720
- essential: yes [1] DEG
⊟Accession numbers[edit | edit source]
- Location: AE005672 (734233..734952) NCBI
- BioCyc:
- MicrobesOnline: 116276 MicrobesOnline
- PneumoBrowse for strain D39V: SPV_0679 PneumoBrowse
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
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661ATGAAGATTGATATTTTAACCCTCTTTCCAGAGATGTTTTCTCCACTGGAGCACTCAATC
GTTGGAAAGGCTCGAGAAAAAGGGCTCTTGGATATCCAGTATCATAATTTTCGAGAAAAT
GCTGAAAAGGCCCGTCATGTAGATGATGAGCCCTACGGAGGCGGTCAGGGCATGTTGCTC
AGAGCACAACCTATTTTCGATTCCTTTGATGCTATTGAAAAGAAAAATCCGCGCGTTATT
CTCCTTGATCCTGCTGGAAAGCAGTTTGATCAGGCTTATGCTGAAGATTTGGCTCAAGAG
GAAGAGCTAATCTTTATCTGTGGGCACTATGAGGGTTATGATGAGCGCATTAAGACCTTG
GTAACAGATGAGATTTCCCTAGGTGACTATGTCCTCACTGGTGGAGAATTGGCAGCTATG
ACCATGATTGATGCTACAGTTCGCCTGATTCCAGAAGTGATTGGCAAGGAGTCTAGCCAC
CAAGATGATAGTTTTTCTTCAGGTCTTTTAGAATATCCTCAGTACACACGTCCCTATGAT
TATCGAGGCATGGTCGTGCCAGATGTATTGATGAGTGGCCACCATGAAAAGATTCGTCAG
TGGCGATTGTACGAGAGTTTAAAGAAAACCTACGAGCGCAGACCGGATTTACTTGAACAT
TATCAACTGACAGTAGAAGAAGAAAAAATGCTGGCAGAAATCAAAGAAAACAAAGAATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SP_0779 [new locus tag: SP_RS03800 ]
- symbol: TrmD
- description: tRNA (guanine-N1)-methyltransferase
- length: 239
- theoretical pI: 4.5841
- theoretical MW: 27633.1
- GRAVY: -0.555649
⊟Function[edit | edit source]
- TIGRFAM: Protein synthesis tRNA and rRNA base modification tRNA (guanine(37)-N(1))-methyltransferase (TIGR00088; EC 2.1.1.228; HMM-score: 304.4)
- TheSEED :
- tRNA (Guanine37-N1) -methyltransferase (EC 2.1.1.31)
Protein Metabolism Protein biosynthesis Ribosome biogenesis bacterial tRNA (Guanine37-N1) -methyltransferase (EC 2.1.1.31)and 1 more - PFAM: SPOUT (CL0098) tRNA_m1G_MT; tRNA (Guanine-1)-methyltransferase (PF01746; HMM-score: 219.1)and 2 moreSPOUT_MTase; Predicted SPOUT methyltransferase (PF02590; HMM-score: 17.8)no clan defined DUF2573; Protein of unknown function (DUF2573) (PF10835; HMM-score: 14)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- DeepLocPro: Cytoplasmic
- Cytoplasmic Score: 0.9993
- Cytoplasmic Membrane Score: 0.0002
- Cell wall & surface Score: 0
- Extracellular Score: 0.0006
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.010634
- TAT(Tat/SPI): 0.00031
- LIPO(Sec/SPII): 0.000676
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKARHVDDEPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEELIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSHQDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLLEHYQLTVEEEKMLAEIKENKE
⊟Experimental data[edit | edit source]
- protein localization:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: SP_0777 > rimM > trmD > SP_0780
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription[edit | edit source]
- transcription start site:
⊟Expression data[edit | edit source]
- PneumoExpress for strain D39V: SPV_0679 PneumoExpress
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You can add further information about the gene and protein here. [edit]
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Jane A Thanassi, Sandra L Hartman-Neumann, Thomas J Dougherty, Brian A Dougherty, Michael J Pucci
Identification of 113 conserved essential genes using a high-throughput gene disruption system in Streptococcus pneumoniae.
Nucleic Acids Res: 2002, 30(14);3152-62
[PubMed:12136097] [WorldCat.org] [DOI] (I p)Tim van Opijnen, Andrew Camilli
A fine scale phenotype-genotype virulence map of a bacterial pathogen.
Genome Res: 2012, 22(12);2541-51
[PubMed:22826510] [WorldCat.org] [DOI] (I p) - ↑ 2.0 2.1 Indu Warrier, Nikhil Ram-Mohan, Zeyu Zhu, Ariana Hazery, Haley Echlin, Jason Rosch, Michelle M Meyer, Tim van Opijnen
The Transcriptional landscape of Streptococcus pneumoniae TIGR4 reveals a complex operon architecture and abundant riboregulation critical for growth and virulence.
PLoS Pathog: 2018, 14(12);e1007461
[PubMed:30517198] [WorldCat.org] [DOI] (I e)