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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 31-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae TIGR4
  • locus tag: SP_1542 [new locus tag: SP_RS07595 ]
  • pan locus tag?: PNEUPAN002826000
  • symbol: asnS
  • pan gene symbol?: asnC
  • synonym:
  • product: asparaginyl-tRNA synthetase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SP_1542 [new locus tag: SP_RS07595 ]
  • symbol: asnS
  • product: asparaginyl-tRNA synthetase
  • replicon: chromosome
  • strand: -
  • coordinates: 1449792..1451135
  • length: 1344
  • essential: yes [1] DEG

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGACAAAACGTGTAACGATTATTGACGTAAAAGACTATGTTGGTCAGGAAGTGACGATT
    GGCGCTTGGGTTGCCAACAAATCAGGAAAAGGAAAAATCGCTTTCTTACAATTGCGTGAT
    GGAACAGCCTTCTTTCAAGGTGTGGCTTTTAAACCAAACTTTGTCGAAAAATTTGGTGAA
    GAAGTGGGACTTGAGAAGTTTGATGTTATCAAACGCTTGAGCCAAGAAACGTCTGTTTAT
    GTGACAGGTATTGTCAAAGAGGACGAACGTTCTAAATTTGGCTATGAGTTGGACATCACA
    GACATCGAAGTGATCGGTGAATCTCAAGACTACCCAATCACACCAAAAGAACACGGAACA
    GACTTTTTGATGGATAACCGTCACTTGTGGCTACGCTCTCGTAAGCAAGTAGCTGTGTTG
    CAAATCCGTAACGCTATTATCTATGCAACTTATGAGTTCTTTGACAAGAACGGTTTTATG
    AAGTTTGACAGCCCAATTCTTTCAGGAAATGCGGCAGAAGATTCTACAGAACTCTTTGAA
    ACTGACTACTTCGGAACGCCAGCCTACTTGAGCCAATCAGGTCAGCTTTACCTAGAAGCA
    GGGGCTATGGCTCTTGGTCGTGTCTTTGACTTTGGTCCAGTTTTCCGTGCTGAAAAATCA
    AAAACACGCCGTCACTTGACTGAGTTCTGGATGATGGATGCTGAGTACTCATACTTGACA
    CATGATGAGTCGCTTGACTTGCAAGAAGCTTATGTGAAAGCTCTTCTACAAGGTGTTCTT
    GACCGCGCGCCTCAAGCCTTGGAAACCTTGGAACGTGATACAGAACTCTTGAAACGCTAC
    ATTGCAGAGCCATTCAAACGTATCACTTACGATCAAGCCATTGACCTCTTGCAAGAGCAT
    GAAAATGATGAAGATGCTGACTACGAGCATCTTGAGCATGGTGATGACTTTGGGTCACCA
    CACGAAACTTGGATTTCAAACCACTTTGGTGTGCCAACATTTGTCATGAACTATCCAGCA
    GCCATCAAGGCCTTCTACATGAAACCAGTTCCTGGAAATCCAGAGCGCGTGCTTTGTGCA
    GACTTGCTTGCTCCAGAAGGCTATGGAGAAATTATCGGTGGGTCTATGCGTGAGGAAGAT
    TACGATGCCCTTGTCGCTAAGATGGATGAACTTGGCATGGATCGTACAGAATATGAATTC
    TACCTTGACCTTCGTAAATACGGTACAGTTCCACACGGAGGATTTGGTATCGGTATCGAA
    CGTATGGTAACCTTCGCAGCAGGAACAAAACATATCCGTGAAGCTATTCCATTCCCACGT
    ATGTTGCACCGTATCAAACCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1344

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SP_1542 [new locus tag: SP_RS07595 ]
  • symbol: AsnS
  • description: asparaginyl-tRNA synthetase
  • length: 447
  • theoretical pI: 4.69334
  • theoretical MW: 51066.4
  • GRAVY: -0.380761

⊟Function[edit | edit source]

  • reaction:
    EC 6.1.1.22?  ExPASy
    Asparagine--tRNA ligase ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn)
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA aminoacylation asparagine--tRNA ligase (TIGR00457; EC 6.1.1.22; HMM-score: 469.8)
    and 5 more
    Genetic information processing Protein synthesis tRNA aminoacylation aspartate--tRNA(Asn) ligase (TIGR00458; EC 6.1.1.23; HMM-score: 275)
    Genetic information processing Protein synthesis tRNA aminoacylation aspartate--tRNA ligase (TIGR00459; EC 6.1.1.-; HMM-score: 122.8)
    Genetic information processing Protein synthesis tRNA aminoacylation lysine--tRNA ligase (TIGR00499; EC 6.1.1.6; HMM-score: 85.5)
    Unknown function General EF-P lysine aminoacylase GenX (TIGR00462; HMM-score: 45.8)
    Genetic information processing Protein synthesis tRNA aminoacylation pyrrolysine--tRNA ligase, C-terminal region (TIGR02367; EC 6.1.1.26; HMM-score: 17)
  • TheSEED  :
    • Asparaginyl-tRNA synthetase (EC 6.1.1.22)
    Protein Metabolism Protein biosynthesis tRNA aminoacylation, Glu and Gln  Asparaginyl-tRNA synthetase (EC 6.1.1.22)
    and 1 more
    RNA Metabolism RNA processing and modification tRNA modification Archaea  Asparaginyl-tRNA synthetase (EC 6.1.1.22)
  • PFAM:
    tRNA_synt_II (CL0040) tRNA-synt_2; tRNA synthetases class II (D, K and N) (PF00152; HMM-score: 271.8)
    and 3 more
    OB (CL0021) tRNA_anti-codon; OB-fold nucleic acid binding domain (PF01336; HMM-score: 51.9)
    tRNA_synt_II (CL0040) tRNA-synt_2d; tRNA synthetases class II core domain (F) (PF01409; HMM-score: 13.1)
    EF_hand (CL0220) EF-hand_13; EF-hand domain (PF17958; HMM-score: 12.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 10
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.997
    • Cytoplasmic Membrane Score: 0.0001
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.003
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003752
    • TAT(Tat/SPI): 0.000308
    • LIPO(Sec/SPII): 0.000577
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTAFFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITDIEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGFMKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRAEKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTELLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPTFVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDELGMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription[edit | edit source]

  • transcription start site:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Jane A Thanassi, Sandra L Hartman-Neumann, Thomas J Dougherty, Brian A Dougherty, Michael J Pucci
    Identification of 113 conserved essential genes using a high-throughput gene disruption system in Streptococcus pneumoniae.
    Nucleic Acids Res: 2002, 30(14);3152-62
    [PubMed:12136097] [WorldCat.org] [DOI] (I p)
  2. ↑ Indu Warrier, Nikhil Ram-Mohan, Zeyu Zhu, Ariana Hazery, Haley Echlin, Jason Rosch, Michelle M Meyer, Tim van Opijnen
    The Transcriptional landscape of Streptococcus pneumoniae TIGR4 reveals a complex operon architecture and abundant riboregulation critical for growth and virulence.
    PLoS Pathog: 2018, 14(12);e1007461
    [PubMed:30517198] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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