PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BM6001

serotype 19F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7465

serotype 1

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 23-APR-2025

⊟Summary

• organism: Streptococcus pneumoniae TIGR4
• locus tag: SP_RS03400 [old locus tag: SP_0695 ]
• pan locus tag^?: PNEUPAN001607000
• symbol: SP_RS03400
• pan gene symbol^?: moeB
• synonym:
• product: HesA/MoeB/ThiF family protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SP_RS03400 [old locus tag: SP_0695 ]
• symbol: SP_RS03400
• product: HesA/MoeB/ThiF family protein
• replicon: chromosome
• strand: +
• coordinates: 664585..665289
• length: 705
• essential: unknown

⊟Accession numbers

• Location: NC_003028 (664585..665289) NCBI
• BioCyc:
• MicrobesOnline: see SP_0695
• PneumoBrowse for strain D39V: SPV_2205 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  
  ATACAGAATAAAACAGTATTAGTATTAGGAGCAGGAGTCTTAGGATGTTATATATCTCTA
  AGTCTAAGTATGTATGGAGTGAGGAAACTTATTGTCGCTGATTACGATATAATAGAACCA
  TCAAATTTAAATAGGCAAATTCTTTATACAGAGTCGGATGTTGGTAAGGAGAAGATTAAT
  GTTCTTTCTGAAAAAATACACAAGTATAATTCAGATGTTCAGGTAGTACCTATTTCTATT
  AAAGTTTCTTCAGTAGAAGAATTAGAAAAAATTGTTGCGGAATATGGGAGTATAGATTTT
  ATCGTTAAAGCAATTGATACGCCCATTGATATTATAAAAATTGTCAATCAATTTGCTGTA
  TCGCATAAGATATCCTACATATCAGGAGGGTTTAATGGATGCTATCTTATTATTGATAAT
  ATATATATCCCTACCATCGGTTCTTGCTTTGGTTGTCGGAATATAAACAAAGATATAAAT
  AAGTACACTTTATCTGATAAGACAAAGTGGCCGACTACACCAGAGATGCCTGCTATTTTG
  GGAGGGATAATGACTAATTTAATAATTAAAATATTTCTGGGATGTTATAATGAAATCCTA
  ATAGATAACGCTTACGTTTATAATATGAGAAATCATGCTCTAAGTCAAGAAAAATATGTT
  CTGGAAAACGGAGAATGTCCAATTTGTAAAAAAATAATAAAGTGA
  60
  120
  180
  240
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  360
  420
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  540
  600
  660
  705
  

⊟Protein

⊟General

• locus tag: SP_RS03400 [old locus tag: SP_0695 ]
• symbol: SP_RS03400
• description: HesA/MoeB/ThiF family protein
• length: 234
• theoretical pI: 7.264
• theoretical MW: 26213.5
• GRAVY: 0.15812

⊟Function

• reaction:
  EC 2.7.7.-?  ExPASy
• TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiazole biosynthesis adenylyltransferase ThiF (TIGR02356; EC 2.7.7.73; HMM-score: 85.8)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiamine biosynthesis protein ThiF (TIGR02354; HMM-score: 70.4)
  and 5 more
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Molybdopterin molybdopterin synthase sulfurylase MoeB (TIGR02355; HMM-score: 67.5)
  ubiquitin-activating enzyme E1 (TIGR01408; HMM-score: 44.3)
  E1-like protein-activating enzyme Gsa7p/Apg7p (TIGR01381; HMM-score: 31)
  PRTRC system ThiF family protein (TIGR03736; HMM-score: 17.6)
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides beta-aspartyl peptidase (TIGR01975; EC 3.4.19.5; HMM-score: 15.9)
• TheSEED: see SP_0695
• PFAM:
  NADP_Rossmann (CL0063) ThiF; ThiF family (PF00899; HMM-score: 122.4)
  and 4 more
  Shikimate_DH; Shikimate / quinate 5-dehydrogenase (PF01488; HMM-score: 15.5)
  FAD_binding_3; FAD binding domain (PF01494; HMM-score: 13.2)
  Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 11.5)
  EspA_CesA (CL0628) T3SchapCesA; Type III secretion system filament chaperone CesA (PF11439; HMM-score: 9.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helix: 1
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.8564
  • Cytoplasmic Membrane Score: 0.0631
  • Cell wall & surface Score: 0.0001
  • Extracellular Score: 0.0803
• SignalP: Signal peptide LIPO(Sec/SPII) length 15 aa
  • SP(Sec/SPI): 0.15649
  • TAT(Tat/SPI): 0.002895
  • LIPO(Sec/SPII): 0.520037
  • Cleavage Site: CS pos: 15-16. VLG-CY. Pr: 0.5157
• predicted transmembrane helices (TMHMM): 3

⊟Accession numbers

• GI:
• RefSeq: WP_078064025 NCBI
• UniProt: see SP_0695

⊟Protein sequence

• MQNKTVLVLGAGVLGCYISLSLSMYGVRKLIVADYDIIEPSNLNRQILYTESDVGKEKINVLSEKIHKYNSDVQVVPISIKVSSVEELEKIVAEYGSIDFIVKAIDTPIDIIKIVNQFAVSHKISYISGGFNGCYLIIDNIYIPTIGSCFGCRNINKDINKYTLSDKTKWPTTPEMPAILGGIMTNLIIKIFLGCYNEILIDNAYVYNMRNHALSQEKYVLENGECPICKKIIK

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SP_RS03400 > SP_RS03405

⊟Regulation

• regulator:

⊟Transcription

• transcription start site:

⊟Expression data

• PneumoExpress for strain D39V: SPV_2205 PneumoExpress

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications