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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 09-APR-2021

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae TIGR4
  • locus tag: SP_RS10825 [old locus tag: SP_2121 ]
  • pan locus tag?: PNEUPAN003699000
  • symbol: SP_RS10825
  • pan gene symbol?: hisS
  • synonym:
  • product: histidine--tRNA ligase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SP_RS10825 [old locus tag: SP_2121 ]
  • symbol: SP_RS10825
  • product: histidine--tRNA ligase
  • replicon: chromosome
  • strand: -
  • coordinates: 2032438..2033727
  • length: 1290
  • essential: yes [1]

⊟Accession numbers[edit | edit source]

  • Location: NC_003028 (2032438..2033727) NCBI
  • BioCyc: SP_RS10825 BioCyc
  • MicrobesOnline: see SP_2121
  • PneumoBrowse for strain D39V: SPV_1950 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGAAATTACAAAAACCAAAAGGAACGCAGGATATTTTACCTGCTGAGTCTGCTAAGTGG
    CAGTACGTTGAGGGCTTTGCCCGTGAGATTTTCAAACGCTACAACTATGCAGAAGTGCGC
    ACGCCTATTTTTGAGCATTACGAGGTTATCAGTCGCTCTGTCGGAGATACAACGGATATC
    GTAACCAAGGAAATGTACGATTTTTATGACAAGGGTGACCGTCATATTACCCTCCGTCCA
    GAAGGAACTGCACCCGTTGTCCGTTCCTATGTGGAAAATAAACTTTTCGCCCCAGAAGTG
    CAAAAGCCAAGCAAGTTCTACTACATGGGACCTATGTTCCGTTATGAGCGTCCACAGGCA
    GGGCGCTTGCGCCAATTCCACCAGATTGGTGTTGAGTGTTTTGGCTCTAGCAATCCAGCT
    ACCGATGTGGAAACAATCGCTATGGCAGCCCATTTTTTGAAGGAAATCGGTATTCAAGGT
    GTCAAATTGCACCTCAACACTCTTGGAAATCCTGAGAGCCGTGCAGCCTACCGCCAAGCC
    TTGATTGACTATTTGACACCGCTCAAGGAGACCTTGTCTAAGGATAGCCAACGTCGCTTG
    GAGGAAAATCCTCTTCGTGTCTTGGACTCTAAGGAAAAAGAAGACAAGGTGGCAGTAGAG
    AATGCGCCGTCTATCTTGGACTTTCTTGATGAAGAAAGCCAAGCTCATTTTGATGCTGTG
    CGTCAGATGTTGGAAAATCTTGGAGTAGATTACATCATCGATACCAATATGGTGCGTGGT
    CTGGACTACTACAACCACACCATTTTCGAGTTTATCACAGAGATTGAGGGCAATGACCTG
    ACCGTCTGTGCGGGTGGTCGCTACGATGGTTTGGTTGCTTACTTTGGAGGCCCTGAAACT
    GCTGGATTTGGTTTTGGACTTGGTGTAGAGCGCCTGCTTCTCATCCTTGAAAAGCAAGGT
    GTGACCCTCCCTATCGAAAACGCCCTAGATGTCTATATCGCAGTCTTGGGCGAAGGGGCA
    AATATCAAGGCCTTGGAATTGGTACAGGCTCTTCGCCAACAAGGTTTCAAAGCAGAGCGT
    GATTACCTCAACCGTAAACTAAAAGCTCAGTTCAAGTCAGCCGATGTCTTTGCGGCTAAG
    ACCCTCATCACCCTAGGAGAGAGCGAAGTCGAAAGCGGACAAGTGACGGTCAAGAACAAC
    CAAACCCGAGAAGAAGTGCAAGTGTCACTTGAGACAATCAGCCAAAACTTCTCAGAAATC
    TTTGAAAAACTAGGATTTTATACTCAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1290

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SP_RS10825 [old locus tag: SP_2121 ]
  • symbol: SP_RS10825
  • description: histidine--tRNA ligase
  • length: 429
  • theoretical pI: 4.93986
  • theoretical MW: 48653.8
  • GRAVY: -0.374126

⊟Function[edit | edit source]

  • reaction:
    EC 6.1.1.21?  ExPASy
    Histidine--tRNA ligase ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His)
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA aminoacylation histidine--tRNA ligase (TIGR00442; EC 6.1.1.21; HMM-score: 516.5)
    and 7 more
    Metabolism Amino acid biosynthesis Histidine family ATP phosphoribosyltransferase, regulatory subunit (TIGR00443; HMM-score: 182.1)
    Genetic information processing Protein synthesis tRNA aminoacylation threonine--tRNA ligase (TIGR00418; EC 6.1.1.3; HMM-score: 34.5)
    Genetic information processing Protein synthesis tRNA aminoacylation pyrrolysine--tRNA ligase, C-terminal region (TIGR02367; EC 6.1.1.26; HMM-score: 26.2)
    Genetic information processing Protein synthesis tRNA aminoacylation phenylalanine--tRNA ligase, alpha subunit (TIGR00468; EC 6.1.1.20; HMM-score: 22.8)
    Genetic information processing Protein synthesis tRNA aminoacylation proline--tRNA ligase (TIGR00408; EC 6.1.1.15; HMM-score: 20.3)
    Genetic information processing Protein synthesis tRNA aminoacylation proline--tRNA ligase (TIGR00409; EC 6.1.1.15; HMM-score: 18.2)
    Genetic information processing Protein synthesis tRNA aminoacylation glycine--tRNA ligase (TIGR00389; EC 6.1.1.14; HMM-score: 14.3)
  • TheSEED: see SP_2121
  • PFAM:
    tRNA_synt_II (CL0040) tRNA-synt_His; Histidyl-tRNA synthetase (PF13393; HMM-score: 153.3)
    and 5 more
    tRNA-synt_2b; tRNA synthetase class II core domain (G, H, P, S and T) (PF00587; HMM-score: 67.9)
    IIaaRS-ABD (CL0458) HGTP_anticodon; Anticodon binding domain (PF03129; HMM-score: 48.4)
    tRNA_synt_II (CL0040) tRNA-synt_2d; tRNA synthetases class II core domain (F) (PF01409; HMM-score: 31.6)
    tRNA-synt_2; tRNA synthetases class II (D, K and N) (PF00152; HMM-score: 20)
    no clan defined PGM_PMM_IV; Phosphoglucomutase/phosphomannomutase, C-terminal domain (PF00408; HMM-score: 13.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 10
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005938
    • TAT(Tat/SPI): 0.000533
    • LIPO(Sec/SPII): 0.000725
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIFEHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQKPSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIAMAAHFLKEIGIQGVKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVAVENAPSILDFLDEESQAHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIEGNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVTLPIENALDVYIAVLGEGANIKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVESGQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Tim van Opijnen, Andrew Camilli
    A fine scale phenotype-genotype virulence map of a bacterial pathogen.
    Genome Res: 2012, 22(12);2541-51
    [PubMed:22826510] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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