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From PneumoWiki
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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 02-MAY-2018

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Expression data
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Streptococcus pneumoniae D39
  • locus tag: SPD_0064 [new locus tag: SPD_RS00325 ]
  • pan locus tag?: PNEUPAN000705000
  • symbol: SPD_0064
  • pan gene symbol?: agaR
  • synonym: cpsR
  • product: transcriptional regulator, GntR family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPD_0064 [new locus tag: SPD_RS00325 ]
  • symbol: SPD_0064
  • product: transcriptional regulator, GntR family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 64781..65524
  • length: 744
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: CP000410 (64781..65524) NCBI
  • BioCyc: see SPD_RS00325
  • MicrobesOnline: 2017015 MicrobesOnline
  • PneumoBrowse for strain D39V: SPV_0064 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGGAGAAGGAAAATAGAGGTAAAAATATGGCAATTCCCAAATATCAATACATAAAAGAC
    GAATTAAAAAATAAAATCATCTCTGGGCAGTTTAGCAGTGGAGATAAATTTTACACTGAA
    GCAGAACTCATTTCAATGTACGATGTCAGCTCTATCACAGTTGTTCGCGCCTTAAACGAC
    CTTGCCAAAGATGGCTATATTGTCCGCCAACAAGGAAAAGGAACCTTCGTTTCACGTGCA
    CGCAAACACAAGCTTGTAGAGTTTTCAGATATTGAACTCTTCAATGCTAAAGATGATAAG
    GTAACTGTTCTTTCAATTGAACGCGGAAACAAGCTTGTTTACTTGGAAAAATTGGGTCTA
    CGCGGCGATCAATTCTACTACAAGATTGAACGTATCCGCGAATCAAACGGTGTCGTTTAT
    ATCTACCATACATCCTACATCCCAGAACAATATATCAACGCAAATTATCCAAACCTTGAA
    TACTATAGTTCTATCTATAATCGTTTCAAGTTAGACTACCACATTCACATGAATGATGAA
    CACTTTGAAGAAATCAACGAAATAGTCTTTCCAACTCCAGAACATGCGGCTTCAGTCCTC
    GGAGTCGATGAACAATTCCCTACTGTCCTACAAACTAAAATCACCAAACTCGAATCAACT
    GGCCAAGTTTTAGAATACAGTGAAACTTACAAACGATCTGATTACTACAAGATTAAATTC
    ATCTCATGTGACCGTGATCACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    744

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPD_0064 [new locus tag: SPD_RS00325 ]
  • symbol: SPD_0064
  • description: transcriptional regulator, GntR family protein
  • length: 247
  • theoretical pI: 6.63969
  • theoretical MW: 29021.6
  • GRAVY: -0.603644

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Metabolism Energy metabolism Amino acids and amines histidine utilization repressor (TIGR02018; HMM-score: 68.6)
    Signal transduction Regulatory functions DNA interactions histidine utilization repressor (TIGR02018; HMM-score: 68.6)
    Signal transduction Regulatory functions DNA interactions trehalose operon repressor (TIGR02404; HMM-score: 66)
    Signal transduction Regulatory functions DNA interactions phosphonate utilization transcriptional regulator PhnR (TIGR03337; HMM-score: 63.1)
    and 6 more
    Metabolism Transport and binding proteins Anions phosphonate metabolism transcriptional regulator PhnF (TIGR02325; HMM-score: 51.2)
    Signal transduction Regulatory functions DNA interactions phosphonate metabolism transcriptional regulator PhnF (TIGR02325; HMM-score: 51.2)
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis fatty acid metabolism transcriptional regulator FadR (TIGR02812; HMM-score: 18.5)
    Metabolism Fatty acid and phospholipid metabolism Degradation fatty acid metabolism transcriptional regulator FadR (TIGR02812; HMM-score: 18.5)
    Signal transduction Regulatory functions DNA interactions fatty acid metabolism transcriptional regulator FadR (TIGR02812; HMM-score: 18.5)
    phosphonate utilization associated transcriptional regulator (TIGR03338; HMM-score: 16.6)
  • TheSEED  :
    • Predicted transcriptional regulator of N-Acetylglucosamine utilization, GntR family
  • ⊞PFAM:
    UTRA (CL0122) UTRA; UTRA domain (PF07702; HMM-score: 60.6)
    HTH (CL0123) GntR; Bacterial regulatory proteins, gntR family (PF00392; HMM-score: 59.1)
    and 7 more
    Costars; Costars (PF14705; HMM-score: 18.3)
    HTH_DeoR; DeoR-like helix-turn-helix domain (PF08220; HMM-score: 15)
    HTH_27; Winged helix DNA-binding domain (PF13463; HMM-score: 14.6)
    MarR; MarR family (PF01047; HMM-score: 14)
    HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 13.8)
    MarR_2; MarR family (PF12802; HMM-score: 12.3)
    Mor; Mor transcription activator family (PF08765; HMM-score: 11.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effector: N-acetylgalactosamine
  • ⊞genes regulated by AgaR/CpsR*, TF important in N-acetylgalactosamine utilizationRegPrecise
    repression
    agaR*
    bgaC > gadV* > gadW* > gadE* > gadF*
    transcription units transferred from TIGR4 data RegPrecise

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005398
    • TAT(Tat/SPI): 0.000302
    • LIPO(Sec/SPII): 0.000544
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: ABJ54247 NCBI
  • UniProt: A0A0H2ZMA3 UniProt

⊟Protein sequence[edit | edit source]

  • MEKENRGKNMAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSITVVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNKLVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRFKLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYSETYKRSDYYKIKFISCDRDH

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

  • MicrobesOnline: no polycistronic organisation predicted

⊟Regulation[edit | edit source]

  • ⊞regulators: AgaR/CpsR* (repression) regulon, CcpA regulon
    AgaR/CpsR*(TF)important in N-acetylgalactosamine utilization; RegPrecise 
    CcpA(TF)important in Global catabolite repression; RegPrecise 

⊟Expression data[edit | edit source]

  • PneumoExpress for strain D39V: SPV_0064 PneumoExpress

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Kaifeng Wu, Hongmei Xu, Yuqiang Zheng, Libin Wang, Xuemei Zhang, Yibing Yin
CpsR, a GntR family regulator, transcriptionally regulates capsular polysaccharide biosynthesis and governs bacterial virulence in Streptococcus pneumoniae.
Sci Rep: 2016, 6;29255
[PubMed:27386955] [WorldCat.org] [DOI] (I e)
Muhammad Afzal, Sulman Shafeeq, Hifza Ahmed, Oscar P Kuipers
N-acetylgalatosamine-Mediated Regulation of the aga Operon by AgaR in Streptococcus pneumoniae.
Front Cell Infect Microbiol: 2016, 6;101
[PubMed:27672623] [WorldCat.org] [DOI] (I e)

Retrieved from "http://pneumowiki.med.uni-greifswald.de/index.php?title=SPD_0064&oldid=6648"
  • This page was last edited on 20 January 2022, at 13:19.
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