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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 25-OCT-2024

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae TCH8431/19A
  • locus tag: HMPREF0837_RS09090 [old locus tag: HMPREF0837_11902 ]
  • pan locus tag?: PNEUPAN003075000
  • symbol: ileS
  • pan gene symbol?: ileS
  • synonym:
  • product: isoleucine--tRNA ligase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: HMPREF0837_RS09090 [old locus tag: HMPREF0837_11902 ]
  • symbol: ileS
  • product: isoleucine--tRNA ligase
  • replicon: chromosome
  • strand: -
  • coordinates: 1741245..1744037
  • length: 2793
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_014251 (1741245..1744037) NCBI
  • BioCyc: HMPREF0837_RS09090 BioCyc
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_1472 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAAACTCAAAGATACCCTTAATCTTGGGAAAACAGCTTTCCCAATGCGTGCAGGCCTT
    CCGACCAAAGAGCCAGTTTGGCAAAAGGAATGGGAAGATGCAAAACTTTACCAACGTCGT
    CAAGAACTAAATGAAGGGAAACCGCATTTTGTCTTGCATGATGGCCCTCCATACGCAAAC
    GGAAATATCCACGTTGGACATGCCATGAATCATATCTCAAAAGATATCATCATTCGTTCT
    AAGTCTATGTCAGGTTTTAACGCACCTTATATCCCAGGTTGGGATACTCATGGTCTGCCA
    ATCGAGCAAGTTTTGGCAAAGCAAGGTGTGAAGCGCAAAGAAATGGACTTGGTTGAGTAC
    TTGAAACTCTGTCGTGAGTACGCTCTTTCTCAAGTTTATAAACAACGTGATGATTTCAAA
    CGCTTGGGGATGTCAGGTGACTGGGAAAATTTATATGTGACTTTAACTCCAGACTATGAA
    GCAGCCCAAATCCGTGTCTTTGGTGAGATGGCTAATAAGGGTTATATCTACCGTGGTGCC
    AAGCCAGTTTACTGGTCATGGTCATCTGAGTCAGCCCTTGCCGAAGCGGAAATTGAATAC
    CATGATTTGGTTTCGACTTCACTTTATTATGCCAATAAGGTAAAAGATGGCAAAGGTATC
    CTAGATACAGATACTTATATCGTAGTCTGGACAACAACTCCATTTACCATCACAGCTTCT
    CGTGGTTTGACTGTTGGTGCAGATATTGATTACGTTTTGGTTCAACCTGCTAGTGAAACT
    CGTAAGTTTGTCGTTGCTGCAGAATTGTTGACTAGCTTGTCTGAGAAATTTGGCTGGGCT
    GATGTTCAAGTGTTGGCAACTTACCGTGGTCAAGAACTCAACCACATCGTAACAGAACAC
    CCATGGGATACAGCTGTAGATGAGTTGGTAATCCTTGGTGATCACGTTACCACTGACTCT
    GGTACAGGTATCGTCCATACAGCCCCTGGTTTTGGTGAGGACGACTACAATGTTGGTATT
    GCTAATGGTCTTGAAGTCGCAGTGACTGTTGACGAACGCGGTATCATGATGGCGAATGCT
    GGTCCTGAGTTTGAAGGTCAGTTCTACGACAAGGTTGTACCAACTGTTATTGAAAAACTT
    GGTAACCTCCTTCTAGCCCAAGAAGAAATCTCTCACTCATACCCATTTGACTGGCGTACT
    AAGAAACCAATCATCTGGCGTGCAGTTCCACAATGGTTTGCCTCAGTTTCAAAATTCCGC
    CAAGAAATCTTGGATGCTATTGATAAAGTTAAATTCCATACAGAATGGGGTAAAGTACGT
    CTATATAATATGATTCGTGACCGTGGTGACTGGGTTATCTCTCGTCAACGTGCTTGGGGT
    GTTCCACTTCCAATCTTCTATGCAGAAGACGGTACAGCTATCATGACAGCTGAGACTATT
    GAACATGTGGCGCAACTCTTTGAAGTACATGGTTCAAGCATTTGGTGGGAACGTGATGCC
    AAAGACCTCTTACCAGAAGGATTTACTCATCCAGGTTCACCAAACGGCGAGTTCAAGAAA
    GAAACAGATATTATGGATGTATGGTTTGACTCAGGTTCATCATGGAATGGTGTTTTGGTA
    AATCGTCCAAATCTGACTTATCCAGCTGACCTTTACCTTGAAGGTTCTGACCAATACCGT
    GGTTGGTTCAACTCATCACTCATTACATCTGTTGCCAACCATGGCGTAGCACCTTACAAA
    CAAATCTTGTCACAAGGTTTTACCCTTGATGGTAAAGGTGAGAAGATGTCTAAATCACTT
    GGAAATACCATTGCTCCAAGTGATGTTGAAAAACAATTTGGTGCAGAAATCTTGCGTCTC
    TGGGTAACAAGTGTTGACTCAAGCAATGACGTGCGTATCTCTATGGATATTTTGAGCCAA
    GTTTCTGAAACTTACCGTAAGATTCGTAACACTCTTCGTTTCTTGATTGCCAATACATCT
    GACTTTAACCCAGCTCAAGATGTAGTCGCTTACGATGAACTTCGTTCAGTTGATAAGTAC
    ATGACGATTCGCTTTAACCAGCTTGTCAAGACCATTCGTGATGCCTATGCGGACTTTGAA
    TTCTTGACAATCTACAAGGCCTTGGTGAACTTTATCAACGTTGACTTGTCAGCCTTCTAC
    CTTGATTTTGCCAAAGATGTTGTTTACATTGAAGGTGCCAAATCATTGGAACGCCGTCAA
    ATGCAGACTGTTTTCTATGACATTCTTGTCAAAATCACCAAACTCTTGACACCAATCCTT
    CCTCACACTGCGGAAGAAATCTGGTCATATCTTGAGTTTGAAGCTGAAGACTTCGTTCAA
    TTGTCAGAATTGCCAGAAGCTCAAACTTTTGCTAATCAAGAAGAAGTCTTGGATACATGG
    GCAGCCTTCATGGACTTCCGTGGACAAGCTCAAAAAGCCTTGGAAGAAGCTCGTAATGCA
    AAAGTTATCGGGAAATCGCTTGAAGCACACTTGACAGTTTATCCAAATGAAGTTGTGAAA
    ACTCTACTCGAAGCAGTAAACAGCAATGTGGCTCAACTTTTGATCGTGTCAGACTTGACC
    ATCGCAGAAGGGCCAGCTCCAGAAGCTGCCCTTAGCTTCGAAGATGTAGCCTTCACAGTT
    GAACGCGCTGCAGGTCAAGTATGTGACCGTTGCCGTCGTATCGACCCAACAACAGCAGAA
    CGCAGCTACCAGGCAGTCATCTGTGACCACTGTGCAAGCATCGTAGAAGAAAATTTTGCG
    GAAGCAGTCGCAGAAGGATTTGAAGAGAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2760
    2793

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: HMPREF0837_RS09090 [old locus tag: HMPREF0837_11902 ]
  • symbol: IleS
  • description: isoleucine--tRNA ligase
  • length: 930
  • theoretical pI: 4.72181
  • theoretical MW: 105121
  • GRAVY: -0.255484

⊟Function[edit | edit source]

  • reaction:
    EC 6.1.1.5?  ExPASy
    Isoleucine--tRNA ligase ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile)
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA aminoacylation isoleucine--tRNA ligase (TIGR00392; EC 6.1.1.5; HMM-score: 924.1)
    and 6 more
    Genetic information processing Protein synthesis tRNA aminoacylation valine--tRNA ligase (TIGR00422; EC 6.1.1.9; HMM-score: 410.3)
    Genetic information processing Protein synthesis tRNA aminoacylation leucine--tRNA ligase (TIGR00396; EC 6.1.1.4; HMM-score: 146.4)
    Genetic information processing Protein synthesis tRNA aminoacylation leucine--tRNA ligase (TIGR00395; EC 6.1.1.4; HMM-score: 145.4)
    Genetic information processing Protein synthesis tRNA aminoacylation methionine--tRNA ligase (TIGR00398; EC 6.1.1.10; HMM-score: 128.3)
    Genetic information processing Protein synthesis tRNA aminoacylation cysteine--tRNA ligase (TIGR00435; EC 6.1.1.16; HMM-score: 42.4)
    Genetic information processing Protein synthesis tRNA aminoacylation lysine--tRNA ligase (TIGR00467; EC 6.1.1.6; HMM-score: 13.7)
  • TheSEED: see HMPREF0837_11902
  • PFAM:
    HUP (CL0039) tRNA-synt_1; tRNA synthetases class I (I, L, M and V) (PF00133; HMM-score: 640.1)
    and 8 more
    DALR (CL0258) Anticodon_1; Anticodon-binding domain of tRNA ligase (PF08264; HMM-score: 92.8)
    HUP (CL0039) tRNA-synt_1g; tRNA synthetases class I (M) (PF09334; HMM-score: 85.4)
    tRNA-synt_1e; tRNA synthetases class I (C) catalytic domain (PF01406; HMM-score: 32.7)
    tRNA-synt_1f; tRNA synthetases class I (K) (PF01921; HMM-score: 29.6)
    Zn_Beta_Ribbon (CL0167) zf-FPG_IleRS; Zinc finger found in FPG and IleRS (PF06827; HMM-score: 23.4)
    no clan defined ArfGap; Putative GTPase activating protein for Arf (PF01412; HMM-score: 12)
    DUF4729; Domain of unknown function (DUF4729) (PF15866; HMM-score: 10.8)
    RTP1_C2; Required for nuclear transport of RNA pol II C-terminus 2 (PF10304; HMM-score: 9.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9982
    • Cytoplasmic Membrane Score: 0
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0017
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.014024
    • TAT(Tat/SPI): 0.001779
    • LIPO(Sec/SPII): 0.002045
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_000768038 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKLKDTLNLGKTAFPMRAGLPTKEPVWQKEWEDAKLYQRRQELNEGKPHFVLHDGPPYANGNIHVGHAMNHISKDIIIRSKSMSGFNAPYIPGWDTHGLPIEQVLAKQGVKRKEMDLVEYLKLCREYALSQVYKQRDDFKRLGMSGDWENLYVTLTPDYEAAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGKGILDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPASETRKFVVAAELLTSLSEKFGWADVQVLATYRGQELNHIVTEHPWDTAVDELVILGDHVTTDSGTGIVHTAPGFGEDDYNVGIANGLEVAVTVDERGIMMANAGPEFEGQFYDKVVPTVIEKLGNLLLAQEEISHSYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDAIDKVKFHTEWGKVRLYNMIRDRGDWVISRQRAWGVPLPIFYAEDGTAIMTAETIEHVAQLFEVHGSSIWWERDAKDLLPEGFTHPGSPNGEFKKETDIMDVWFDSGSSWNGVLVNRPNLTYPADLYLEGSDQYRGWFNSSLITSVANHGVAPYKQILSQGFTLDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTSVDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDVVAYDELRSVDKYMTIRFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQMQTVFYDILVKITKLLTPILPHTAEEIWSYLEFEAEDFVQLSELPEAQTFANQEEVLDTWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIVSDLTIAEGPAPEAALSFEDVAFTVERAAGQVCDRCRRIDPTTAERSYQAVICDHCASIVEENFAEAVAEGFEEK

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription[edit | edit source]

  • transcription start site:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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