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serotype 4
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D39V
serotype 2
Hungary19A-6
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EF3030
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670-6B
serotype 6B
6A-10
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70585
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A026
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A66
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AP200
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ATCC 49619
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BM6001
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BVJ1JL
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CGSP14
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G54
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HU-OH
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Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 25-NOV-2024

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae R6
  • locus tag: SPR_RS07460 [old locus tag: spr1502 ]
  • pan locus tag?: PNEUPAN003075000
  • symbol: ileS
  • pan gene symbol?: ileS
  • synonym:
  • product: isoleucine--tRNA ligase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPR_RS07460 [old locus tag: spr1502 ]
  • symbol: ileS
  • product: isoleucine--tRNA ligase
  • replicon: chromosome
  • strand: -
  • coordinates: 1480465..1483257
  • length: 2793
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAAACTCAAAGACACCCTTAATCTTGGGAAAACTGAATTCCCAATGCGTGCAGGCCTT
    CCTACCAAAGAGCCAGTTTGGCAAAAGGAATGGGAATATGCAAAACTTTATCAACGTCGT
    CAAGAATTGAACCAAGGAAAACCTCATTTCACCTTGCATGATGGCCCTCCATACGCTAAC
    GGAAATATCCACGTTGGACATGCTATGAACAAGATTTCAAAAGATATCATTGTTCGTTCT
    AAGTCTATGTCAGGATTTTACGCACCATTTATTCCTGGTTGGGATACTCATGGTCTGCCA
    ATCGAGCAAGTCTTGTCAAAACAAGGTGTCAAACGTAAAGAAATGGACTTGGTTGAGTAC
    TTGAAACTTTGCCGTGAGTACGCTCTTTCTCAAGTAGATAAACAACGTGAAGATTTTAAA
    CGTTTGGGTGTTTCTGGTGACTGGGAAAATCCATATGTGACCTTGACTCCTGACTATGAA
    GCAGCTCAAATTCGTGTATTTGGTGAGATGGCTAATAAGGGTTATATCTACCGTGGTGCT
    AAGCCAGTTTACTGGTCATGGTCATCTGAGTCAGCCCTTGCTGAAGCAGAGATTGAATAC
    CATGACTTGGTTTCAACTTCCCTTTACTATGCCAACAAGGTAAAAGATGGCAAAGGAGTT
    CTAGATACAGATACTTATATCGTTGTCTGGACAACGACTCCATTTACTATCACAGCCTCT
    CGTGGTTTGACGGTTGGTGCAGATATTGATTACGTTTTGGTTCAACCTGCTGGTGAAGCT
    CGTAAGTTTGTCGTTGCTGCTGAATTATTGACTAGCTTGTCTGAGAAATTTGGCTGGGCT
    GATGTTCAAGTTTTGGAAACTTACCGTGGCCAAGAACTCAACCACATCGTAACAGAACAC
    CCATGGGATACAGCTGTAGAAGAGTTGGTAATTCTTGGTGACCACGTTACGACTGACTCT
    GGTACAGGTATTGTCCATACAGCCCCTGGTTTTGGTGAGGACGATTACAATGTTGGTATT
    GCTAATAATCTTGAAGTCGCAGTGACTGTTGATGAACGTGGTATCATGATGAAGAATGCT
    GGTCCTGAATTTGAAGGTCAATTCTATGAAAAGGTAGTTCCAACTGTTATTGAAAAACTT
    GGTAACCTCCTTCTTGCCCAAGAAGAAATCTCTCACTCATATCCATTTGACTGGCGTACT
    AAGAAACCAATCATCTGGCGTGCAGTTCCACAATGGTTTGCCTCAGTTTCTAAATTCCGT
    CAAGAAATCTTGGACGAAATTGAAAAAGTGAAATTCCACTCAGAATGGGGTAAAGTCCGT
    CTTTACAATATGATCCGTGACCGTGGTGACTGGGTTATCTCTCGTCAACGTGCTTGGGGT
    GTTCCACTTCCTATCTTCTACGCTGAAGATGGTACAGCTATCATGGTAGCTGAAACTATT
    GAACACGTAGCTCAACTTTTTGAAGAACATGGTTCAAGCATTTGGTGGGAACGTGATGCC
    AAAGACCTCTTGCCAGAAGGATTTACTCATCCAGGTTCACCAAACGGCGAGTTCAAAAAA
    GAAACTGATATCATGGACGTTTGGTTTGACTCAGGTTCATCATGGAATGGAGTGGTGGTA
    AACCGTCCTGAATTGACTTACCCAGCCGACCTTTACCTAGAAGGTTCTGACCAATACCGT
    GGTTGGTTTAACTCATCACTTATCACATCTGTTGCCAACCATGGCGTAGCACCTTACAAA
    CAAATCTTGTCACAAGGTTTTGCCCTTGATGGTAAAGGTGAGAAGATGTCTAAATCTCTT
    GGAAATACTATTGCTCCAAGCGATGTTGAAAAACAATTCGGTGCTGAAATCTTGCGTCTC
    TGGGTAACAAGTGTTGACTCAAGTAATGACGTGCGTATCTCTATGGATATCTTGAGCCAA
    GTTTCTGAAACTTACCGTAAGATTCGTAACACTCTTCGTTTCTTGATTGCCAATACATCT
    GACTTTAACCCAGCTCAAGATACAGTCGCTTACGATGAGCTTCGTTCAGTTGATAAGTAC
    ATGACGATTCGCTTTAACCAGCTTGTCAAGACCATTCGTGATGCCTATGCAGACTTTGAA
    TTCTTGACGATCTACAAGGCCTTGGTGAACTTTATCAACGTTGACTTGTCAGCCTTCTAC
    CTTGATTTTGCCAAAGATGTTGTTTACATTGAAGGTGCCAAATCACTGGAACGCCGTCAA
    ATGCAGACTGTCTTCTATGACATTCTTGTCAAAATCACCAAACTCTTGACACCAATCCTT
    CCTCACACTGCGGAAGAAATCTGGTCATATCTTGAGTTTGAAACAGAAGACTTCGTCCAA
    TTGTCAGAATTACCAGAAGTTCAAACTTTTGCTAACCAAGAAGAAATCTTGGATACATGG
    GCAGCCTTCATGGACTTTCGTGGACAAGCACAAAAAGCCTTGGAAGAAGCTCGTAATGCA
    AAAGTTATCGGTAAATCACTTGAAGCACACTTGACAGTTTATCCAAATGAAGTTGTGAAA
    ACTCTACTCGAAGCAGTAAACAGCAATGTAGCACAACTTTTGATCGTGTCTGAGTTGACC
    ATCGCAGAAGGACCAGCTCCGGAAGCTGCCCTTAGCTTCGAAGATGTAGCCTTCACAGTT
    GAACGTGCTACTGGTGAAGTATGTGACCGTTGCCGTCGTATCGACCCAACAACAGCAGAA
    CGCAGCTACCAGGCAGTTATCTGTGACCACTGTGCAAGCATCGTAGAAGAAAACTTTGCG
    GAAGCAGTCGCAGAAGGATTTGAAGAGAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2760
    2793

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPR_RS07460 [old locus tag: spr1502 ]
  • symbol: IleS
  • description: isoleucine--tRNA ligase
  • length: 930
  • theoretical pI: 4.66478
  • theoretical MW: 105474
  • GRAVY: -0.292688

⊟Function[edit | edit source]

  • reaction:
    EC 6.1.1.5?  ExPASy
    Isoleucine--tRNA ligase ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile)
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA aminoacylation isoleucine--tRNA ligase (TIGR00392; EC 6.1.1.5; HMM-score: 930.7)
    and 6 more
    Genetic information processing Protein synthesis tRNA aminoacylation valine--tRNA ligase (TIGR00422; EC 6.1.1.9; HMM-score: 416.7)
    Genetic information processing Protein synthesis tRNA aminoacylation leucine--tRNA ligase (TIGR00396; EC 6.1.1.4; HMM-score: 144.6)
    Genetic information processing Protein synthesis tRNA aminoacylation leucine--tRNA ligase (TIGR00395; EC 6.1.1.4; HMM-score: 138.6)
    Genetic information processing Protein synthesis tRNA aminoacylation methionine--tRNA ligase (TIGR00398; EC 6.1.1.10; HMM-score: 127.4)
    Genetic information processing Protein synthesis tRNA aminoacylation cysteine--tRNA ligase (TIGR00435; EC 6.1.1.16; HMM-score: 41.3)
    Genetic information processing Protein synthesis tRNA aminoacylation lysine--tRNA ligase (TIGR00467; EC 6.1.1.6; HMM-score: 14)
  • TheSEED: see spr1502
  • PFAM:
    HUP (CL0039) tRNA-synt_1; tRNA synthetases class I (I, L, M and V) (PF00133; HMM-score: 650.8)
    and 7 more
    DALR (CL0258) Anticodon_1; Anticodon-binding domain of tRNA ligase (PF08264; HMM-score: 92.7)
    HUP (CL0039) tRNA-synt_1g; tRNA synthetases class I (M) (PF09334; HMM-score: 84.8)
    tRNA-synt_1e; tRNA synthetases class I (C) catalytic domain (PF01406; HMM-score: 33.6)
    tRNA-synt_1f; tRNA synthetases class I (K) (PF01921; HMM-score: 28.2)
    Zn_Beta_Ribbon (CL0167) zf-FPG_IleRS; Zinc finger found in FPG and IleRS (PF06827; HMM-score: 24.8)
    Vps51 (CL0295) COG2; COG (conserved oligomeric Golgi) complex component, COG2 (PF06148; HMM-score: 12.3)
    no clan defined RTP1_C2; Required for nuclear transport of RNA pol II C-terminus 2 (PF10304; HMM-score: 10)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9985
    • Cytoplasmic Membrane Score: 0
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0014
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.012761
    • TAT(Tat/SPI): 0.004588
    • LIPO(Sec/SPII): 0.00205
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKLKDTLNLGKTEFPMRAGLPTKEPVWQKEWEYAKLYQRRQELNQGKPHFTLHDGPPYANGNIHVGHAMNKISKDIIVRSKSMSGFYAPFIPGWDTHGLPIEQVLSKQGVKRKEMDLVEYLKLCREYALSQVDKQREDFKRLGVSGDWENPYVTLTPDYEAAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGKGVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPAGEARKFVVAAELLTSLSEKFGWADVQVLETYRGQELNHIVTEHPWDTAVEELVILGDHVTTDSGTGIVHTAPGFGEDDYNVGIANNLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLLAQEEISHSYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKVKFHSEWGKVRLYNMIRDRGDWVISRQRAWGVPLPIFYAEDGTAIMVAETIEHVAQLFEEHGSSIWWERDAKDLLPEGFTHPGSPNGEFKKETDIMDVWFDSGSSWNGVVVNRPELTYPADLYLEGSDQYRGWFNSSLITSVANHGVAPYKQILSQGFALDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTSVDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDTVAYDELRSVDKYMTIRFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQMQTVFYDILVKITKLLTPILPHTAEEIWSYLEFETEDFVQLSELPEVQTFANQEEILDTWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIVSELTIAEGPAPEAALSFEDVAFTVERATGEVCDRCRRIDPTTAERSYQAVICDHCASIVEENFAEAVAEGFEEK

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription[edit | edit source]

  • transcription start site:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Jane A Thanassi, Sandra L Hartman-Neumann, Thomas J Dougherty, Brian A Dougherty, Michael J Pucci
    Identification of 113 conserved essential genes using a high-throughput gene disruption system in Streptococcus pneumoniae.
    Nucleic Acids Res: 2002, 30(14);3152-62
    [PubMed:12136097] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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