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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 22-NOV-2024

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae gamPNI0373
  • locus tag: HMPREF1038_RS04850 [old locus tag: HMPREF1038_00985 ]
  • pan locus tag?: PNEUPAN001963000
  • symbol: HMPREF1038_RS04850
  • pan gene symbol?: lytB
  • synonym:
  • product: glucosaminidase domain-containing protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: HMPREF1038_RS04850 [old locus tag: HMPREF1038_00985 ]
  • symbol: HMPREF1038_RS04850
  • product: glucosaminidase domain-containing protein
  • replicon: chromosome
  • strand: +
  • coordinates: 896033..898330
  • length: 2298
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: NC_018630 (896033..898330) NCBI
  • BioCyc: HMPREF1038_RS04850 BioCyc
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_0853 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAATTTAGGAGAATTTTGGTACAATAAAATAAATAAGAACAGAGGAAGAAGGTTAATG
    AAGAAAGTAAGATTTATTTTTTTAGCTCTGCTATTTTTCTTAGCTAGTCCAGAGGGTGCA
    ATGGCTAGTGATGGTACTTGGCAAGGAAAACAGTATCTGAAAGAAGATGGCAGTCAAGCA
    GCGAATGAGTGGGTTTTTGATACTCATTATCAATCTTGGTTCTATATAAAAGCAGATGCT
    AACTATACTGAAAATGAATGGCTAAAGCAAGGTGACGACTATTTTTACCTCAAATCTGGT
    GGCTATATGGCCAAATCAGAATGGGTAGAAGACAAGGGAGCCTTTTATTATCTTGACCAA
    GATGGAAAGATGAAAAGAAATGCTTGGGTAGGAACTTCCTATGTTGGTGCAACAGGTGCC
    AAAGTAATAGAAGACTGGGTCTATGATTCTCAATACGATGCTTGGTTTTATATCAAAGCA
    GATGGACAGCACGCAGAGAAAGAATGGCTCCAAATTAAAGGGAAGGACTATTATTTCAAA
    TCCGGTGGTTATCTACTAACAAGTCAGTGGATTAATCAAGCTTATGTGAATGCTAGTGGT
    GCCAAAGTACAGCAAGGTTGGCTTTTTGACAAACAATACCAATCTTGGTTTTACATAAAA
    GAAAATGGAAACTATGCTGATAAAGAATGGATTTTCGAGAATGGTCACTATTATTATCTA
    AAATCTGGTGGCTACATGACAGCCAATGAATGGATTTGGGATAAGGAATCTTGGTTTTAT
    CTCAAATTTGATGGGAAAATAGCTGAAAAAGAATGGGTCTACGATTCTCATAGTCAAGCC
    TGGTACTACTTCAAATCCGGTGGTTACATGACAGCCAATGAATGGATTTGGGATAAGGAA
    TCTTGGTTTTACCTCAAGTCTGATGGCAAAATGGCTGAAAAAGAATGGGTCTACGATTCT
    CATAGTCAAGCCTGGTACTACTTCAAATCTGGTGGCTACATGACAGCCAATGAATGGATT
    TGGGATAAGGAATCTTGGTTTTACCTCAAGTCTGATGGCAAAATGGCTGAAAAAGAATGG
    GTCTACGATTCTCATAGTCAAGCCTGGTACTACTTCAAATCTGGTGGCTACATGACAGCC
    AATGAATGGATTTGGGATAAGGAATCTTGGTTTTACCTCAAGTCTGATGGCAAAATGGCT
    GAAAAAGAATGGGTCTACGATTCTCATAGTCAAGCTTGGTACTACTTCAAATCCGGTGGT
    TACATGACAGCCAATGAATGGATTTGGGATAAGGAATCTTGGTTTTACCTCAAGTCTGAT
    GGCAAAATGGCTGAAAAAGAATGGGTCTACGATTCTCATAGTCAAGCCTGGTACTACTTC
    AAATCTGGTGGTTACATGGCGAAAAATGAGACAGTAGATGGTTATCAGCTTGGAAGCGAT
    GGTAAATGGCTTGGAGGAAAAGCTACAAATGAAAATGCTGCTTACTATCAAGTAGTGCCT
    GTTACAGCCAATGTTTATGATTCAGATGGTGAAAAGCTTTCCTATATATCGCAAGGTAGT
    GTCGTATGGCTAGATAAGGATAGAAAAAGTGATGACAAGCGCTTGGCTATTACTATTTCT
    GGTTTGTCAGGCTATATGAAAACAGAAGATTTACAAGCGCTAGATGCTAGTAAGGACTTT
    ATCCCTTATTATGAGAGTGATGGCCACCGTTTTTATCACTATGTGGCTCAGAATGCTAGT
    ATCCCAGTAGCTTCTCATCTTTCTGATATGGAAGTAGGCAAGAAATATTATTCGGCAGAT
    GGCCTGCATTTTGATGGTTTTAAGCTTGAGAATCCCTTCCTTTTCAAAGATTTAACAGAG
    GCTACAAACTACAGTGCTGAAGAATTGGATAAGGTATTTAGTTTGCTAAACATTAACAAT
    AGCCTTTTGGAGAACAAGGGCGCTACTTTTAAGGAAGCCGAAGAACATTACCATATCAAT
    GCTCTTTATCTCCTTGCCCATAGTGCCCTAGAAAGTAACTGGGGAAGAAGTAAAATTGCC
    AAAGATAAGAATAATTTCTTTGGCATTACAGCCTATGATACGACCCCTTACCTTTCTGCT
    AAGACATTTGATGATGTGGATAAGGGAATTTTAGGTGCAACCAAGTGGATTAAGGAAAAT
    TATATCGATAGGGGAAGAACTTTCCTTGGAAACAAGGCTTCTGGTATGAATGTGGAATAT
    GCTTCAGACCCTTATTGGGGCGAAAAAATTGCTAGTGTGATGATGAAAATCAATGAGAAG
    CTAGGTGGCAAAGATTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2298

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: HMPREF1038_RS04850 [old locus tag: HMPREF1038_00985 ]
  • symbol: HMPREF1038_RS04850
  • description: glucosaminidase domain-containing protein
  • length: 765
  • theoretical pI: 5.19243
  • theoretical MW: 89772.1
  • GRAVY: -0.725882

⊟Function[edit | edit source]

  • TIGRFAM:
    glucan-binding repeat (TIGR04035; HMM-score: 29.7)
    and 1 more
    flagellar rod assembly protein/muramidase FlgJ (TIGR02541; HMM-score: 18.9)
  • TheSEED: see HMPREF1038_00985
  • PFAM:
    Choline_binding (CL0694) Choline_bind_1; Putative cell wall binding repeat (PF01473; HMM-score: 190.8)
    Choline_bind_2; Choline-binding repeat (PF19085; HMM-score: 181)
    and 5 more
    no clan defined WW_like; Peptidoglycan hydrolase LytB WW-like domain (PF17890; HMM-score: 110.4)
    Choline_binding (CL0694) Choline_bind_3; Choline-binding repeat (PF19127; HMM-score: 79.5)
    Lysozyme (CL0037) Glucosaminidase; Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase (PF01832; HMM-score: 70.9)
    no clan defined LytB_WW; Endo-beta-N-acetylglucosaminidase LytB WW domain (PF18342; HMM-score: 62.5)
    SH3 (CL0010) DUF5776; Domain of unknown function (DUF5776) (PF19087; HMM-score: 19.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0.01
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.18
    • Extracellular Score: 9.72
    • Internal Helices: 0
  • DeepLocPro: Extracellular
    • Cytoplasmic Score: 0.0068
    • Cytoplasmic Membrane Score: 0.005
    • Cell wall & surface Score: 0.2726
    • Extracellular Score: 0.7156
  • SignalP: Signal peptide SP(Sec/SPI) length 42 aa
    • SP(Sec/SPI): 0.986228
    • TAT(Tat/SPI): 0.005506
    • LIPO(Sec/SPII): 0.00141
    • Cleavage Site: CS pos: 42-43. AMA-SD. Pr: 0.9778
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_014947327 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASDGTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYTENEWLKQGDDYFYLKSGGYMAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKADGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFYIKENGNYADKEWIFENGHYYYLKSGGYMTANEWIWDKESWFYLKFDGKIAEKEWVYDSHSQAWYYFKSGGYMTANEWIWDKESWFYLKSDGKMAEKEWVYDSHSQAWYYFKSGGYMTANEWIWDKESWFYLKSDGKMAEKEWVYDSHSQAWYYFKSGGYMTANEWIWDKESWFYLKSDGKMAEKEWVYDSHSQAWYYFKSGGYMTANEWIWDKESWFYLKSDGKMAEKEWVYDSHSQAWYYFKSGGYMAKNETVDGYQLGSDGKWLGGKATNENAAYYQVVPVTANVYDSDGEKLSYISQGSVVWLDKDRKSDDKRLAITISGLSGYMKTEDLQALDASKDFIPYYESDGHRFYHYVAQNASIPVASHLSDMEVGKKYYSADGLHFDGFKLENPFLFKDLTEATNYSAEELDKVFSLLNINNSLLENKGATFKEAEEHYHINALYLLAHSALESNWGRSKIAKDKNNFFGITAYDTTPYLSAKTFDDVDKGILGATKWIKENYIDRGRTFLGNKASGMNVEYASDPYWGEKIASVMMKINEKLGGKD

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

P García, M P González, E García, R López, J L García
LytB, a novel pneumococcal murein hydrolase essential for cell separation.
Mol Microbiol: 1999, 31(4);1275-81
[PubMed:10096093] [WorldCat.org] [DOI] (P p)Blanca De Las Rivas, José L García, Rubens López, Pedro García
Purification and polar localization of pneumococcal LytB, a putative endo-beta-N-acetylglucosaminidase: the chain-dispersing murein hydrolase.
J Bacteriol: 2002, 184(18);4988-5000
[PubMed:12193614] [WorldCat.org] [DOI] (P p)Xiao-Hui Bai, Hui-Jie Chen, Yong-Liang Jiang, Zhensong Wen, Yubin Huang, Wang Cheng, Qiong Li, Lei Qi, Jing-Ren Zhang, Yuxing Chen, Cong-Zhao Zhou
Structure of pneumococcal peptidoglycan hydrolase LytB reveals insights into the bacterial cell wall remodeling and pathogenesis.
J Biol Chem: 2014, 289(34);23403-16
[PubMed:25002590] [WorldCat.org] [DOI] (I p)

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