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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 02-MAY-2018

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae D39
  • locus tag: SPD_0190 [new locus tag: SPD_RS01055 ]
  • pan locus tag?: PNEUPAN000941000
  • symbol: nrdG
  • pan gene symbol?: nrdG
  • synonym:
  • product: anaerobic ribonucleoside-triphosphate reductase activating protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPD_0190 [new locus tag: SPD_RS01055 ]
  • symbol: nrdG
  • product: anaerobic ribonucleoside-triphosphate reductase activating protein
  • replicon: chromosome
  • strand: +
  • coordinates: 194080..194670
  • length: 591
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGAATAATCCAAAACCACAAGAATGGAAAAGCGAGGAACTTAGTCAAGGTCGTATCATT
    GACTACAAGGCCTTTAACTTTGTGGACGGCGAAGGCGTGCGCAACTCTCTCTATGTATCA
    GGCTGTATGTTTCACTGCGAGGGATGTTATAATGTTGCGACTTGGTCTTTTAATGCTGGC
    ATTCCCTATACAGCAGAATTAGAAGAGCAGATTATGGCAGACCTTGCCCAACCCTATGTT
    CAAGGCTTGACTTTGCTGGGAGGGGAGCCTTTTCTCAATACTGGCATTCTCTTGCCTCTA
    GTTAAACGCATCCGAAAGGAATTGCCAGACAAGGACATTTGGTCCTGGACGGGCTACACT
    TGGGAAGAAATGATGTTGGAAACTCCAGATAAACTGGAACTCTTATCATTGATTGACATT
    CTTGTCGATGGAAGATATGATCGAACTAAGAGAAATCTCATGCTCCAGTTTCGAGGTTCG
    TCCAACCAACGAATTATCGATGTGCAAAAATCGCTCAAAAGTGGGCAAGTAGTGATTTGG
    GACAAGCTCAATGACGGAAAAGAAAGCTATGAACAGGTGAAGAGAGAATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    591

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPD_0190 [new locus tag: SPD_RS01055 ]
  • symbol: NrdG
  • description: anaerobic ribonucleoside-triphosphate reductase activating protein
  • length: 196
  • theoretical pI: 4.71159
  • theoretical MW: 22634.7
  • GRAVY: -0.458673

⊟Function[edit | edit source]

  • reaction:
    EC 1.97.1.4?  ExPASy
    [Formate-C-acetyltransferase]-activating enzyme S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical
  • TIGRFAM:
    Genetic information processing Protein fate Protein modification and repair anaerobic ribonucleoside-triphosphate reductase activating protein (TIGR02491; EC 1.97.1.-; HMM-score: 198.5)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides 2'-Deoxyribonucleotide metabolism anaerobic ribonucleoside-triphosphate reductase activating protein (TIGR02491; EC 1.97.1.-; HMM-score: 198.5)
    and 13 more
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides 2'-Deoxyribonucleotide metabolism anaerobic ribonucleoside-triphosphate reductase activating protein (TIGR02826; EC 1.97.1.-; HMM-score: 50.6)
    Genetic information processing Protein fate Protein modification and repair pyruvate formate-lyase 1-activating enzyme (TIGR02493; EC 1.97.1.4; HMM-score: 48)
    Metabolism Energy metabolism Anaerobic pyruvate formate-lyase 1-activating enzyme (TIGR02493; EC 1.97.1.4; HMM-score: 48)
    glycyl-radical enzyme activating protein (TIGR02494; EC 1.97.1.-; HMM-score: 34.2)
    Genetic information processing Protein fate Protein modification and repair glycine radical enzyme activase, YjjW family (TIGR04041; EC 1.97.1.-; HMM-score: 33.9)
    Genetic information processing Protein fate Protein modification and repair anaerobic ribonucleoside-triphosphate reductase activating protein (TIGR02495; EC 1.97.-.-; HMM-score: 27.8)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides 2'-Deoxyribonucleotide metabolism anaerobic ribonucleoside-triphosphate reductase activating protein (TIGR02495; EC 1.97.-.-; HMM-score: 27.8)
    Genetic information processing Protein synthesis tRNA and rRNA base modification putative 7-cyano-7-deazaguanosine (preQ0) biosynthesis protein QueE (TIGR03963; HMM-score: 22.1)
    Metabolism Energy metabolism Amino acids and amines choline TMA-lyase-activating enzyme (TIGR04395; EC 1.97.-.-; HMM-score: 21.3)
    Genetic information processing Protein fate Protein modification and repair [benzylsuccinate synthase]-activating enzyme (TIGR04003; EC 1.97.-.-; HMM-score: 17)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin heme d1 biosynthesis radical SAM protein NirJ (TIGR04051; HMM-score: 14.3)
    His-Xaa-Ser system radical SAM maturase HxsC (TIGR03977; HMM-score: 13.7)
    putative 7-cyano-7-deazaguanosine (preQ0) biosynthesis protein QueE (TIGR04349; HMM-score: 12.4)
  • TheSEED  :
    • Anaerobic ribonucleoside-triphosphate reductase-activating protein
    Nucleosides and Nucleotides Nucleosides and Nucleotides - no subcategory Ribonucleotide reduction  Ribonucleotide reductase of class III (anaerobic), activating protein (EC 1.97.1.4)
  • PFAM:
    4Fe-4S (CL0344) Fer4_12; 4Fe-4S single cluster domain (PF13353; HMM-score: 172.8)
    and 2 more
    Fer4_14; 4Fe-4S single cluster domain (PF13394; HMM-score: 103.4)
    TIM_barrel (CL0036) Radical_SAM; Radical SAM superfamily (PF04055; HMM-score: 24.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.249824
    • TAT(Tat/SPI): 0.001033
    • LIPO(Sec/SPII): 0.004118
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMFHCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVKRIRKELPDKDIWSWTGYTWEEMMLETPDKLELLSLIDILVDGRYDRTKRNLMLQFRGSSNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: NrdR* (repression) regulon, ComX1 regulon
    NrdR*(TF)important in Deoxyribonucleotide biosynthesis; RegPrecise    transcription unit transferred from TIGR4 data RegPrecise 
    ComX1(sigma factor)controls the expression of late competence genes [1]

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Jelle Slager, Rieza Aprianto, Jan-Willem Veening
    Refining the Pneumococcal Competence Regulon by RNA Sequencing.
    J Bacteriol: 2019, 201(13);
    [PubMed:30885934] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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