⊟Summary[edit | edit source]
- organism: Streptococcus pneumoniae D39
- locus tag: SPD_RS08210 [old locus tag: SPD_1543 ]
- pan locus tag?: PNEUPAN003141000
- symbol: SPD_RS08210
- pan gene symbol?: phpP
- synonym:
- product: Stp1/IreP family PP2C-type Ser/Thr phosphatase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SPD_RS08210 [old locus tag: SPD_1543 ]
- symbol: SPD_RS08210
- product: Stp1/IreP family PP2C-type Ser/Thr phosphatase
- replicon: chromosome
- strand: -
- coordinates: 1565612..1566352
- length: 741
- essential: unknown
⊟Accession numbers[edit | edit source]
- Location: NC_008533 (1565612..1566352) NCBI
- BioCyc: G1G6V-1678 BioCyc
- MicrobesOnline: see SPD_1543
- PneumoBrowse for strain D39V: SPV_1543 PneumoBrowse
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
61
121
181
241
301
361
421
481
541
601
661
721ATGGAAATTTCATTATTAACAGATGTTGGTCAGAAACGAACAAATAACCAAGACTATGTC
AACCACTATGTCAATAGAGCTGGACGTACCATGATTATTTTAGCTGATGGGATGGGAGGT
CATCGCGCAGGGAATATCGCTAGTGAAATGGCGGTCACAGACCTGGGTGTAGCTTGGGTT
GATACCCAGATCGATACAGTCAATGAAGTGCGTGAATGGTTCGCCCATTACCTAGAAATT
GAAAATCAAAAGATTCACCAGCTTGGTCAGGATGAAGCTTACAGAGGCATGGGAACTACT
TTGGAAGTCCTTGCTATTATTGATAATCAGGCTATCTATGCTCATATTGGTGATTCGCGT
ATCGGCTTGATTCGTGGAGAAGAATACCATCAGTTGACGAGCGATCATTCCTTGGTTAAT
GAATTGCTCAAGGCTGGTCAATTGACACCAGAAGAGGCAGAAGCTCATCCGCAAAAAAAT
ATTATCACCCAGTCTATTGGGCAAAAAGATGAAATTCAGCCTGATTTTGGGACAGTTATC
CTTGAGTCAGGTGACTATCTCTTGCTCAATAGTGACGGCTTGACCAACATGATTTCAGGC
AGTGAGATTCGTGATATTGTAACCAGTGATATTCCTTTAGCAGATAAAACGGAGACACTT
GTTCGTTTTGCTAACAATGCAGGAGGTTTAGACAACATTACGGTTGCCCTTGTTTCTATG
AACGAGGAGGATGCAGAATGA60
120
180
240
300
360
420
480
540
600
660
720
741
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SPD_RS08210 [old locus tag: SPD_1543 ]
- symbol: SPD_RS08210
- description: Stp1/IreP family PP2C-type Ser/Thr phosphatase
- length: 246
- theoretical pI: 4.19099
- theoretical MW: 27103
- GRAVY: -0.296341
⊟Function[edit | edit source]
- TIGRFAM: Transcription Degradation of RNA regulator of ribonuclease activity A (TIGR02998; HMM-score: 13)Regulatory functions Protein interactions regulator of ribonuclease activity A (TIGR02998; HMM-score: 13)
- TheSEED: see SPD_1543
- PFAM: PP2C (CL0238) PP2C_2; Protein phosphatase 2C (PF13672; HMM-score: 61.3)and 3 morePP2C; Protein phosphatase 2C (PF00481; HMM-score: 39.8)SpoIIE; Stage II sporulation protein E (SpoIIE) (PF07228; HMM-score: 18.5)no clan defined Suppressor_APC; Adenomatous polyposis coli tumour suppressor protein (PF11414; HMM-score: 14.7)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.007105
- TAT(Tat/SPI): 0.001069
- LIPO(Sec/SPII): 0.000464
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAGNIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLEVLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKNIITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTETLVRFANNAGGLDNITVALVSMNEEDAE
⊟Experimental data[edit | edit source]
- protein localization:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: pknB < SPD_RS08210 < rsmB < SPD_RS08220 < SPD_RS08225 < SPD_RS08230 < gmk
⊟Regulation[edit | edit source]
- regulator:
⊟Expression data[edit | edit source]
- PneumoExpress for strain D39V: SPV_1543 PneumoExpress
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
⊟Relevant publications[edit | edit source]
Linda Nováková, Lenka Sasková, Petra Pallová, Jirí Janecek, Jana Novotná, Ales Ulrych, Jose Echenique, Marie-Claude Trombe, Pavel Branny
Characterization of a eukaryotic type serine/threonine protein kinase and protein phosphatase of Streptococcus pneumoniae and identification of kinase substrates.
FEBS J: 2005, 272(5);1243-54
[PubMed:15720398] [WorldCat.org] [DOI] (P p)Makoto Osaki, Tania Arcondéguy, Amandine Bastide, Christian Touriol, Hervé Prats, Marie-Claude Trombe
The StkP/PhpP signaling couple in Streptococcus pneumoniae: cellular organization and physiological characterization.
J Bacteriol: 2009, 191(15);4943-50
[PubMed:19502404] [WorldCat.org] [DOI] (I p)
Ulrych A, Holečková N, Goldová J, Doubravová L, Benada O, Kofroňová O, Halada P, Branny P. Characterization of pneumococcal Ser/Thr protein phosphatase phpP mutant and identification of a novel PhpP substrate, putative RNA binding protein Jag. BMC Microbiol. 2016 Oct 24;16(1):247. doi: 10.1186/s12866-016-0865-6. PMID: 27776484; PMCID: PMC5078927.
Ulrych A, Fabrik I, Kupčík R, Vajrychová M, Doubravová L, Branny P. Cell Wall Stress Stimulates the Activity of the Protein Kinase StkP of Streptococcus pneumoniae, Leading to Multiple Phosphorylation. J Mol Biol. 2021 Dec 3;433(24):167319. doi: 10.1016/j.jmb.2021.167319. Epub 2021 Oct 21. PMID: 34688688.