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PangenomeTIGR4
serotype 4
D39serotype 2
D39Vserotype 2
Hungary19A-6serotype 19A
EF3030serotype 19F
670-6Bserotype 6B
6A-10serotype 6A
70585serotype 5
A026serotype 19F
A66serotype 3
AP200serotype 11A
ASP0581serotype 12F
ATCC 49619serotype 19F
ATCC 700669serotype 23F
BVJ1JLserotype 1
CGSP14serotype 14
G54serotype 19F
HU-OHserotype 3
Hu15serotype 19A
Hu17serotype 19A
INV104serotype 1
INV200serotype 14
JJAserotype 14
MDRSPN001serotype 19F
NCTC7466serotype 2
NU83127serotype 4
OXC141serotype 3
P1031serotype 1
R6serotype 2
SP49serotype 19A
SPN032672serotype 1
SPN034156serotype 3
SPN034183serotype 3
SPN994038serotype 3
SPN994039serotype 3
SPNA45serotype 3
ST556serotype 19F
TCH8431/19Aserotype 19A
Taiwan19F-14serotype 19F
Xen35serotype 4
gamPNI0373serotype 1
NCBI: 31-JAN-2014
⊟Summary[edit | edit source]
- organism: Streptococcus pneumoniae Hungary19A-6
- locus tag: SPH_0474 [new locus tag: SPH_RS02305 ]
- pan locus tag?: PNEUPAN001225000
- symbol: SPH_0474
- pan gene symbol?: aliA
- synonym:
- product: oligopeptide binding protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SPH_0474 [new locus tag: SPH_RS02305 ]
- symbol: SPH_0474
- product: oligopeptide binding protein
- replicon: chromosome
- strand: +
- coordinates: 422552..424534
- length: 1983
- essential: unknown
⊟Accession numbers[edit | edit source]
- Location: CP000936 (422552..424534) NCBI
- BioCyc: see SPH_RS02305
- MicrobesOnline: 5695323 MicrobesOnline
- PneumoBrowse for strain D39V: SPV_0334 PneumoBrowse
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
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121
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421
481
541
601
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1021
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1981ATGAAAAGTTCAAAACTACTTGCCCTTGCGGGCGTGACATTATTGGCGGCGACTACTTTA
GCTGCATGCTCTGGATCAGGTTCAAGCGCTAAAGGTGAGAAGACATTCTCATACATTTAT
GAGACAGACCCTGATAACCTCAACTATTTGACAACTGCTAAGGCTGCGACAGCAAATATT
ACCAGTAACGTGGTTGATGGTTTGCTAGAAAATGATCGCTACGGGAACTTTGTGCCGTCT
ATGGCTGAGGATTGGTCTGTATCCAAGGATGGATTGACTTACACTTATACTATCCGTAAG
GATGCAAAATGGTATACTTCTGAAGGTGAAGAATACGCGGCAGTCAAAGCTCAAGACTTT
GTAACAGGACTAAAATATGCTGCTGATAAAAAATCAGATGCTCTTTACCTTGTTCAAGAA
TCAATCAAAGGGTTGGATGCCTATGTAAAAGGGGAAATCAAAGATTTCTCACAAGTAGGA
ATTAAGGCTCTGGATGAACAGACAGTTCAGTACACTTTGAACAAACCAGAAAGCTTCTGG
AATTCTAAGACAACCATGGGTGTGCTTGCGCCAGTTAATGAAGAGTTTTTGAATTCAAAA
GGAGATGATTTTGCCAAAGCTACGGATCCAAGTAGTCTCTTGTATAACGGTCCTTATTTG
TTGAAATCCATTGTGACCAAATCTTCTGTTGAATTTGCGAAAAATCCGAACTACTGGGAT
AAGGACAATGTGCATATTGACAAAGTTAAATTGTCATTCTGGGATGGTCAAGATACCAGC
AAACCTGCAGAAAACTTTAAAGATGGTAGCCTTACAGCAGCTCGTCTCTATCCAACAAGT
GCAAGTTTCGCAGAGCTTGAGAAGAGTATTAAGGACAATATTGTCTATACTCAACAAGAC
TCTATTACGTATCTAGTTGGTACAAATATTGACCGTCAGTCCTATAAATACACATCTAAG
ACCAGCGACGAACAAAAGGCATCGACTAAAAAGGCTCTCTTAAACAAGGATTTCCGTCAG
GCTATTGCCTTTGGTTTTGATCGTACAGCCTATGCCTCTCAGTTGAATGGACAAACTGGA
GCAAGCAAAATCTTACGTAATATCTTTGTTCCACCAACATTTGTTCAAGCAGATGGTAAA
AACTTTGGCGATATGGTCAAAGAGAAATTGGTCACTTATGGGGATGAATGGAAGGATGTT
AATCTTGCAGATTCTCAGGATGGTCTTTACAATCCAGAAAAAGCCAAGGCTGAATTTGCT
AAAGCTAAATCAGCCTTACAAGCAGAAGGTGTGACTTTCCCAATTCATTTGGATATGCCA
GTTGACCAGACAGCAACTACAAAAGTTCAGCGCGTCCAATCTATGAAACAATCCTTGGAA
GCAACTTTAGGAGCTGATAATGTCATTATTGATATCCAACAACTACAAAAAGACGAAGTA
AACAATATTACATATTTTGCTGAAAATGCTGCTGGCGAAGACTGGGATTTATCAGATAAT
GTCGGTTGGGGTCCAGACTTTGCCGATCCATCAACCTACCTTGATATTATCAAACCTTCT
GTAGGAGAAAGTACTAAAACATATTTAGGGTTTGACTCAGGGGAAGATAATGTAGCTGCT
AAAAAAGTAGGTCTATATGACTACGAAAAATTGGTTACTGAGGCTGGTGATGAGGCTACA
GATGTTGCTAAACGCTATGATAAATACGCTGCAGCCCAAGCTTGGTTGACAGATAGTGCT
TTGATTATTCCAACTACATCTCGTACAGGGCGTCCAATCTTGTCTAAGATGGTACCATTT
ACAATACCATTTGCATTGTCAGGAAATAAAGGTACAAGTGAACCAGTCTTGTATAAATAC
TTGGAACTTCAAGACAAGGCAGTCACTGTAGATGAATACCAAAAAGCTCAGGAAAAATGG
ATGAAAGAAAAAGAAGAGTCTAATAAAAAGGCTCAAGAAGATCTCGCAAAACATGTGAAA
TAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SPH_0474 [new locus tag: SPH_RS02305 ]
- symbol: SPH_0474
- description: oligopeptide binding protein
- length: 660
- theoretical pI: 4.75494
- theoretical MW: 72954
- GRAVY: -0.518333
⊟Function[edit | edit source]
- TIGRFAM: Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, nickel/metallophore periplasmic binding protein (TIGR02294; HMM-score: 41.1)
- TheSEED :
- Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein OppA (TC 3.A.1.5.1)
- PFAM: PBP (CL0177) SBP_bac_5; Bacterial extracellular solute-binding proteins, family 5 Middle (PF00496; HMM-score: 161.3)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cellwall
- Cytoplasmic Score: 0.01
- Cytoplasmic Membrane Score: 0.53
- Cellwall Score: 8.75
- Extracellular Score: 0.7
- Internal Helix: 1
- SignalP: Signal peptide LIPO(Sec/SPII) length 22 aa
- SP(Sec/SPI): 0.007058
- TAT(Tat/SPI): 0.000279
- LIPO(Sec/SPII): 0.992385
- Cleavage Site: CS pos: 22-23. LAA-CS. Pr: 0.9930
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MKSSKLLALAGVTLLAATTLAACSGSGSSAKGEKTFSYIYETDPDNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDAKWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVGIKALDEQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGPYLLKSIVTKSSVEFAKNPNYWDKDNVHIDKVKLSFWDGQDTSKPAENFKDGSLTAARLYPTSASFAELEKSIKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALLNKDFRQAIAFGFDRTAYASQLNGQTGASKILRNIFVPPTFVQADGKNFGDMVKEKLVTYGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQRVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFADPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRYDKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPVLYKYLELQDKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK
⊟Experimental data[edit | edit source]
- protein localization:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator: CodY (repression) regulon
CodY (TF) important in Amino acid metabolism; RegPrecise
⊟Expression data[edit | edit source]
- PneumoExpress for strain D39V: SPV_0334 PneumoExpress
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
⊟Relevant publications[edit | edit source]
J P Claverys, B Grossiord, G Alloing
Is the Ami-AliA/B oligopeptide permease of Streptococcus pneumoniae involved in sensing environmental conditions?
Res Microbiol: 2000, 151(6);457-63
[PubMed:10961459] [WorldCat.org] [DOI] (P p)A R Kerr, P V Adrian, S Estevão, R de Groot, G Alloing, J-P Claverys, T J Mitchell, P W M Hermans
The Ami-AliA/AliB permease of Streptococcus pneumoniae is involved in nasopharyngeal colonization but not in invasive disease.
Infect Immun: 2004, 72(7);3902-6
[PubMed:15213133] [WorldCat.org] [DOI] (P p)Fauzy Nasher, Manfred Heller, Lucy J Hathaway
Streptococcus pneumoniae Proteins AmiA, AliA, and AliB Bind Peptides Found in Ribosomal Proteins of Other Bacterial Species.
Front Microbiol: 2017, 8;2688
[PubMed:29379482] [WorldCat.org] [DOI] (P e)