⊟Summary[edit | edit source]
- organism: Streptococcus pneumoniae SPN032672
- locus tag: SPN032672_RS08140
- pan locus tag?: PNEUPAN001225000
- symbol: SPN032672_RS08140
- pan gene symbol?: aliA
- synonym:
- product: peptide ABC transporter substrate-binding protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SPN032672_RS08140
- symbol: SPN032672_RS08140
- product: peptide ABC transporter substrate-binding protein
- replicon: chromosome
- strand: +
- coordinates: 1513290..1515272
- length: 1983
- essential: unknown
⊟Accession numbers[edit | edit source]
- Location: NC_021003 (1513290..1515272) NCBI
- BioCyc:
- MicrobesOnline:
- PneumoBrowse for strain D39V: SPV_0334 PneumoBrowse
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1981ATGAAAAGTTCAAAACTACTTGCCCTTGCGGGCGTGACATTATTGGCGGCGACTACTTTA
GCTGCATGCTCTGGATCAGGTTCAAGCACTAAAGGTGAGAAGACATTCTCATACATTTAT
GAGACAGACCCTGATAACCTCAACTATTTGACAACTGCTAAGGCTGCGACAGCAAATATT
ACCAGTAACGTGGTTGATGGTTTGCTAGAAAATGATCGCTACGGGAACTTTGTGCCGTCT
ATGGCTGAGGATTGGTCTGTATCCAAGGATGGATTGACTTACACTTATACTATCCGTAAG
GATGCAAAATGGTATACTTCTGAAGGTGAAGAATACGCGGCAGTCAAAGCTCAAGACTTT
GTAACAGGACTAAAATATGCTGCTGATAAAAAATCAGATGCTCTTTACCTTGTTCAAGAA
TCAATCAAAGGGTTGGATGCCTATGTAAAAGGGGAAATCAAAGATTTCTCACAAGTAGGA
ATTAAGGCTTTGGATGATCAGACAGTTCAGTACACTTTGAACAAACCAGAAAGCTTCTGG
AATTCTAAGACAACCATGGGTGTGCTTGCGCCAGTTAATGAAGAGTTTTTGAACTCAAAA
GGGGATGATTTTGCCAAAGCGACGGATCCAAGTAGTCTCTTGTATAACGGTCCTTATTTG
TTGAAATCCATTGTGACAAAATCCTCTGTTGAATTTGCGAAAAATCCGAACTACTGGGAT
AAGGACAATGTGCATGTTGACAAAGTTAAATTGTCATTCTGGGATGGTCAAGATACCAGC
AAACCTGCAGAAAACTTTAAAGATGGTAGCCTTACAGCAGCTCGTCTCTATCCAACAAGT
GCAAGTTTCGCAGAGCTTGAGAAGAGTATGAAGGACAATATTGTCTATACTCAACAAGGC
TCTATTACGTATCTAGTTGGTACAAATATTGACCGTCAGTCCTATAAATATACATCTAAG
ACCAGCGACGAACAAAAGGCATCGACTAAAAAGGCTCTCTTAAACAAGGATTTCCGTCAG
GCTATTGCCTTTGGTTTTGATCGTACAGCCTATGCCTCTCAGTTGAATGGACAAACTGGA
GCAAGCAAAATCTTACGTAATATCTTTGTTCCACCAACATTTGTTCAAGCAGATGGTAAA
AACTTTGGCGATATGGTCAAAGAGAAATTGGTCACTTATGGGGATGAATGGAAGGATGTT
AATCTTGCAGATTCTCAGGATGGTCTTTACAATCCAGAAAAAGCCAAGGCTGAATTTGCT
AAAGCTAAATCAGCCTTACAAGCAGAAGGTGTGACTTTCCCAATTCATTTGGATATGCCA
GTTGACCAGACAGCAACTACAAAAGTTCAGCGCGTCCAATCTATGAAACAATCCTTGGAA
GCAACTTTAGGAGCTGATAATGTCATTATTGATATCCAACAACTACAAAAAGACGAAGTA
AACAATATTACATATTTTGCTGAAAATGCTGCTGGCGAAGACTGGGATTTATCAGATAAT
GTCGGTTGGGGTCCAGACTTTGCCGATCCATCAACCTACCTTGATATTATCAAACCTTCT
GTAGGAGAAAGTACTAAAACATATTTAGGGTTTGACTCAGGGGAAGATAATGTAGCTGCT
AAAAAAGTAGGTCTATATGACTACGAAAAATTGGTCACTGAGGCTGGTGATGAGGCTACA
GATGTTGCTAAACGCTATGATAAATACGCTGCAGCCCAAGCTTGGTTGACAGATAGTGCT
TTGATTATTCCAACTACATCTCGTACAGGGCGCCCAATCTTGTCTAAGATGGTACCATTT
ACAATACCATTTGCATTGTCAGGAAATAAAGGTACAAGTGAACCAGTCTTGTATAAATAC
TTGGAACTTCAAGACAAGGCAGTCACTGTAGATGAATACCAAAAAGCTCAGGAAAAATGG
ATGAAAGAAAAAGAAGAGTCTAATAAAAAGGCTCAAGAAGATCTCGCAAAACATGTGAAA
TAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SPN032672_RS08140
- symbol: SPN032672_RS08140
- description: peptide ABC transporter substrate-binding protein
- length: 660
- theoretical pI: 4.78578
- theoretical MW: 72916
- GRAVY: -0.521818
⊟Function[edit | edit source]
- TIGRFAM: Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, nickel/metallophore periplasmic binding protein (TIGR02294; HMM-score: 39.6)
- TheSEED: data available for D39, Hungary19A-6, TIGR4
- PFAM: PBP (CL0177) SBP_bac_5; Bacterial extracellular solute-binding proteins, family 5 Middle (PF00496; HMM-score: 162)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cellwall
- Cytoplasmic Score: 0.01
- Cytoplasmic Membrane Score: 0.53
- Cellwall Score: 8.75
- Extracellular Score: 0.7
- Internal Helix: 1
- SignalP: Signal peptide LIPO(Sec/SPII) length 22 aa
- SP(Sec/SPI): 0.001574
- TAT(Tat/SPI): 0.000211
- LIPO(Sec/SPII): 0.997966
- Cleavage Site: CS pos: 22-23. LAA-CS. Pr: 0.9984
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI:
- RefSeq: WP_000842605 NCBI
- UniProt:
⊟Protein sequence[edit | edit source]
- MKSSKLLALAGVTLLAATTLAACSGSGSSTKGEKTFSYIYETDPDNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDAKWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVGIKALDDQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGPYLLKSIVTKSSVEFAKNPNYWDKDNVHVDKVKLSFWDGQDTSKPAENFKDGSLTAARLYPTSASFAELEKSMKDNIVYTQQGSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALLNKDFRQAIAFGFDRTAYASQLNGQTGASKILRNIFVPPTFVQADGKNFGDMVKEKLVTYGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQRVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFADPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRYDKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPVLYKYLELQDKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK
⊟Experimental data[edit | edit source]
- protein localization:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- data available for TIGR4
⊟Expression data[edit | edit source]
- PneumoExpress for strain D39V: SPV_0334 PneumoExpress
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
⊟Relevant publications[edit | edit source]
J P Claverys, B Grossiord, G Alloing
Is the Ami-AliA/B oligopeptide permease of Streptococcus pneumoniae involved in sensing environmental conditions?
Res Microbiol: 2000, 151(6);457-63
[PubMed:10961459] [WorldCat.org] [DOI] (P p)A R Kerr, P V Adrian, S Estevão, R de Groot, G Alloing, J-P Claverys, T J Mitchell, P W M Hermans
The Ami-AliA/AliB permease of Streptococcus pneumoniae is involved in nasopharyngeal colonization but not in invasive disease.
Infect Immun: 2004, 72(7);3902-6
[PubMed:15213133] [WorldCat.org] [DOI] (P p)Fauzy Nasher, Manfred Heller, Lucy J Hathaway
Streptococcus pneumoniae Proteins AmiA, AliA, and AliB Bind Peptides Found in Ribosomal Proteins of Other Bacterial Species.
Front Microbiol: 2017, 8;2688
[PubMed:29379482] [WorldCat.org] [DOI] (P e)