From PneumoWiki
Jump to navigation Jump to search
PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 31-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae Hungary19A-6
  • locus tag: SPH_2087 [new locus tag: SPH_RS10205 ]
  • pan locus tag?: PNEUPAN003528000
  • symbol: SPH_2087
  • pan gene symbol?: lytA
  • synonym:
  • product: autolysin

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPH_2087 [new locus tag: SPH_RS10205 ]
  • symbol: SPH_2087
  • product: autolysin
  • replicon: chromosome
  • strand: -
  • coordinates: 1920562..1921335
  • length: 774
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGCAGGTAGGACCTGTTGATAATGGTGCCTGGGACGTTGGGGGCGGTTGGAATGCTGAG
    ACCTATGCAGCGGTTGAACTGATTGAAAGCCATTCAACTAAAGAAGAGTTCATGACGGAC
    TACCGCCTTTATATCGAACTCTTACGCAATCTAGCAGATGAAGCAGGTTTGCCGAAAACG
    CTTGATACAGGGAGTTTAGCTGGAATTAAAACGCACGAGTATTGCACGAATAACCAACCA
    AACAACCACTCAGACCATGTGGATCCATACCCTTACTTGGCAAAATGGGGCATTAGCCGT
    GAGCAGTTTAAGCATGATATTGAGAACGGCTTGACGATTGAAACAGGCTGGCAGAAGAAT
    GACACTGGCTACTGGTACGTACATTCAGACGGCTCTTATCCAAAAGACAAGTTTGAGAAA
    ATCAATGGCACTTGGTACTACTTTGACAGTTCAGGCTATATGCTTGCAGACCGCTGGAGG
    AAGCACACAGACGGCAACTGGTACTGGTTCGACAACTCAGGCGAAATGGCTACAGGCTGG
    AAGAAAATCGCTGATAAGTGGTACTATTTCAACGAAGAAGGTGCCATGAAGACAGGCTGG
    GTCAAGTACAAGGACACTTGGTACTACTTAGACGCTAAAGAAGGCGCCATGGTATCAAAT
    GCCTTTATCCAGTCAGCGGACGGAACAGGCTGGTACTACCTCAAACCAGACGGAACACTG
    GCAGACAAGCCAGAATTCACAGTAGAGCCAGATGGCTTGATTACAGTAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    774

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPH_2087 [new locus tag: SPH_RS10205 ]
  • symbol: SPH_2087
  • description: autolysin
  • length: 257
  • theoretical pI: 4.59912
  • theoretical MW: 29649.5
  • GRAVY: -0.795331

⊟Function[edit | edit source]

  • reaction:
    EC 3.5.1.28?  ExPASy
    N-acetylmuramoyl-L-alanine amidase Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides
  • TIGRFAM:
    glucan-binding repeat (TIGR04035; HMM-score: 57.5)
    and 1 more
    Signal transduction Regulatory functions Protein interactions exported signaling peptide, YydF/SAG_2028 family (TIGR04077; HMM-score: 8.3)
  • TheSEED  :
    • Cell wall-associated murein hydrolase LytA
    Regulation and Cell signaling Programmed Cell Death and Toxin-antitoxin Systems Fratricide in Streptococcus  Cell wall-associated murein hydrolase LytA
  • PFAM:
    Choline_binding (CL0694) Choline_bind_3; Choline-binding repeat (PF19127; HMM-score: 101)
    Choline_bind_1; Putative cell wall binding repeat (PF01473; HMM-score: 93.2)
    and 1 more
    Choline_bind_2; Choline-binding repeat (PF19085; HMM-score: 25.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0.01
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.18
    • Extracellular Score: 9.72
    • Internal Helices: 0
  • DeepLocPro: Extracellular
    • Cytoplasmic Score: 0.0585
    • Cytoplasmic Membrane Score: 0.0004
    • Cell wall & surface Score: 0.0425
    • Extracellular Score: 0.8985
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005672
    • TAT(Tat/SPI): 0.000221
    • LIPO(Sec/SPII): 0.000604
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDYRLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGISREQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLADRWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEGAMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription[edit | edit source]

  • transcription start site:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

P García, M Paz González, E García, J L García, R López
The molecular characterization of the first autolytic lysozyme of Streptococcus pneumoniae reveals evolutionary mobile domains.
Mol Microbiol: 1999, 33(1);128-38
[PubMed:10411730] [WorldCat.org] [DOI] (P p)P Balachandran, S K Hollingshead, J C Paton, D E Briles
The autolytic enzyme LytA of Streptococcus pneumoniae is not responsible for releasing pneumolysin.
J Bacteriol: 2001, 183(10);3108-16
[PubMed:11325939] [WorldCat.org] [DOI] (P p)E Díaz, R López, J L García
Role of the major pneumococcal autolysin in the atypical response of a clinical isolate of Streptococcus pneumoniae.
J Bacteriol: 1992, 174(17);5508-15
[PubMed:1355082] [WorldCat.org] [DOI] (P p)Peter Mellroth, Robert Daniels, Alice Eberhardt, Daniel Rönnlund, Hans Blom, Jerker Widengren, Staffan Normark, Birgitta Henriques-Normark
LytA, major autolysin of Streptococcus pneumoniae, requires access to nascent peptidoglycan.
J Biol Chem: 2012, 287(14);11018-29
[PubMed:22334685] [WorldCat.org] [DOI] (I p)J V Höltje, A Tomasz
Specific recognition of choline residues in the cell wall teichoic acid by the N-acetylmuramyl-L-alanine amidase of Pneumococcus.
J Biol Chem: 1975, 250(15);6072-6
[PubMed:238995] [WorldCat.org] (P p)A M Berry, R A Lock, D Hansman, J C Paton
Contribution of autolysin to virulence of Streptococcus pneumoniae.
Infect Immun: 1989, 57(8);2324-30
[PubMed:2568343] [WorldCat.org] [DOI] (P p)J F Garcia-Bustos, A Tomasz
Teichoic acid-containing muropeptides from Streptococcus pneumoniae as substrates for the pneumococcal autolysin.
J Bacteriol: 1987, 169(2);447-53
[PubMed:2879828] [WorldCat.org] [DOI] (P p)A Tomasz, P Moreillon, G Pozzi
Insertional inactivation of the major autolysin gene of Streptococcus pneumoniae.
J Bacteriol: 1988, 170(12);5931-4
[PubMed:2903859] [WorldCat.org] [DOI] (P p)L V Howard, H Gooder
Specificity of the autolysin of Streptococcus (Diplococcus) pneumoniae.
J Bacteriol: 1974, 117(2);796-804
[PubMed:4149515] [WorldCat.org] [DOI] (P p)P Usobiaga, F J Medrano, M Gasset, J L Garciá, J L Saiz, G Rivas, J Laynez, M Menéndez
Structural organization of the major autolysin from Streptococcus pneumoniae.
J Biol Chem: 1996, 271(12);6832-8
[PubMed:8636107] [WorldCat.org] [DOI] (P p)

Retrieved from "http://pneumowiki.med.uni-greifswald.de/index.php?title=SPH_2087&oldid=13006"