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Hu17
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INV200
serotype 14
JJA
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MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
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serotype 3
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SP49
serotype 19A
SPN032672
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serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
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TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 31-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae JJA
  • locus tag: SPJ_0161 [new locus tag: SPJ_RS00795 ]
  • pan locus tag?: PNEUPAN000805000
  • symbol: SPJ_0161
  • pan gene symbol?: tsaD
  • synonym:
  • product: O-sialoglycoprotein endopeptidase (Glycoprotease)

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPJ_0161 [new locus tag: SPJ_RS00795 ]
  • symbol: SPJ_0161
  • product: O-sialoglycoprotein endopeptidase (Glycoprotease)
  • replicon: chromosome
  • strand: +
  • coordinates: 144546..145556
  • length: 1011
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAGGATAGATATATTTTAGCATTTGAGACATCCTGTGATGAGACCAGTGTCGCCGTC
    TTGAAAAACGACGATGAGCTCTTGTCCAATGTCATTGCTAGTCAAATTGAGAGTCACAAA
    CGTTTTGGTGGCGTAGTGCCCGAAGTAGCCAGTCGTCACCATGTCGAGGTCATTACAGCC
    TGTATCGAGGAGGCATTGGCAGAAGCAGGGATTACCGAAGAGGACGTGACAGCTGTTGCG
    GTTACCTACGGACCAGGCTTGGTCGGAGCCTTGCTAGTTGGTTTGTCAGCTGCCAAGGCC
    TTTGCTTGGGCTCACGGACTTCCACTGATTCCTGTTAATCACATGGCTGGGCACCTCATG
    GCAGCTCAGAGTGTGGAGCCTTTGGAGTTTCCCTTGCTAGCCCTCTTGGTTAGCGGTGGG
    CACACAGAGTTGGTCTATGTTTCGGAGGCAGGAGATTACAAGATTGTTGGGGAGACACGA
    GACGATGCAGTTGGGGAGGCTTATGACAAGGTCGGTCGTGTCATGGGCTTGACCTATCCT
    GCAGGTCGTGAGATTGACGAGCTGGCTCATCAGGGGCAGGATATTTATGATTTCCCCCGT
    GCCATGATTAAGGAAGATAATCTGGAGTTCTCCTTCTCAGGTTTGAAATCTGCCTTTATC
    AATCTTCATCACAATGCCGAGCAAAAGGGAGAAAGCCTGTCTACAGAAGATTTGTGTGCT
    TCCTTCCAAGCAGCAGTTATGGACATTCTCATGGCAAAAACCAAGAAGGCTTTGGAGAAA
    TATCCTGTTAAAACCCTAGTTGTGGCAGGTGGTGTGGCAGCCAATAAAGGTCTCAGAGAA
    CGCCTAGCAGCCGAAATCACAGATGTCAAGGTTATCATCCCCCCTCTGCGACTCTGCGGA
    GACAATGCAGGTATGATTGCCTATGCCAGCGTCAGCGAGTGGAACAAAGAAAACTTCGCA
    GGCTGGGACCTCAATGCCAAACCAAGTCTTGCCTTTGATACCATGGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1011

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPJ_0161 [new locus tag: SPJ_RS00795 ]
  • symbol: SPJ_0161
  • description: O-sialoglycoprotein endopeptidase (Glycoprotease)
  • length: 336
  • theoretical pI: 4.56594
  • theoretical MW: 36173
  • GRAVY: 0.0642857

⊟Function[edit | edit source]

  • reaction:
    EC 3.4.24.57?  ExPASy
    O-sialoglycoprotein endopeptidase Hydrolysis of O-sialoglycoproteins; cleaves 31-Arg-|-Asp-32 bond in glycophorin A. Does not cleave unglycosylated proteins, desialylated glycoproteins or glycoproteins that are only N-glycosylated
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA threonylcarbamoyl adenosine modification protein TsaD (TIGR03723; HMM-score: 431.9)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides metallohydrolase, glycoprotease/Kae1 family (TIGR00329; HMM-score: 351.4)
    and 4 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair universal archaeal protein Kae1 (TIGR03722; HMM-score: 208.5)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA threonylcarbamoyl adenosine modification protein YeaZ (TIGR03725; HMM-score: 63.8)
    putative CoA-substrate-specific enzyme activase (TIGR00241; HMM-score: 16)
    Genetic information processing Protein fate Protein modification and repair carbamoyltransferase HypF (TIGR00143; HMM-score: 14.5)
  • TheSEED  :
    • TsaD/Kae1/Qri7 protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA
    Cell Division and Cell Cycle Cell Division and Cell Cycle - no subcategory YgjD and YeaZ  TsaD/Kae1/Qri7 protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA
    and 2 more
    RNA Metabolism RNA processing and modification YrdC-YciO  TsaD/Kae1/Qri7 protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA
    RNA Metabolism RNA processing and modification YrdC-YciO-Sua5 and associated protein families  TsaD/Kae1/Qri7 protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA
  • PFAM:
    Actin_ATPase (CL0108) TsaD; tRNA N6-adenosine threonylcarbamoyltransferase (PF00814; HMM-score: 350.8)
    and 3 more
    Carbam_trans_N; Carbamoyltransferase N-terminus (PF02543; HMM-score: 29.9)
    BcrAD_BadFG; BadF/BadG/BcrA/BcrD ATPase family (PF01869; HMM-score: 25.9)
    FGGY_C; FGGY family of carbohydrate kinases, C-terminal domain (PF02782; HMM-score: 14.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.983
    • Cytoplasmic Membrane Score: 0.0023
    • Cell wall & surface Score: 0.0018
    • Extracellular Score: 0.0129
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00337
    • TAT(Tat/SPI): 0.000656
    • LIPO(Sec/SPII): 0.000856
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGGVVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFAWAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETRDDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSAFINLHHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANKGLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDTME

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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