PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 13-DEC-2020

⊟Summary

• organism: Streptococcus pneumoniae ATCC 700669
• locus tag: SPN23F_RS11430 [old locus tags: SPN23F22260 SPN23F_22260 ]
• pan locus tag^?: PNEUPAN003926000
• symbol: hk06
• pan gene symbol^?: hk06
• synonym:
• product: two-component system sensor histidine kinase HK06

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SPN23F_RS11430 [old locus tags: SPN23F22260 SPN23F_22260 ]
• symbol: hk06
• product: two-component system sensor histidine kinase HK06
• replicon: chromosome
• strand: -
• coordinates: 2173496..2174827
• length: 1332
• essential: unknown

⊟Accession numbers

• Location: NC_011900 (2173496..2174827) NCBI
• BioCyc: SPN23F_RS11430 BioCyc
• MicrobesOnline: see SPN23F22260
• PneumoBrowse for strain D39V: SPV_2019 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  
  ATGATAAAAAATCCTAAATTATTAACCAAGTCCTTTTTAAGAAGTTTTGCAATTCTAGGT
  GGTGTTGGTCTAGTCATTCATATAGCTATTTATTTGACCTTTCCTTTTTATTATATTCAA
  CTGGAGGGGGAAAAGTTTAATGAGAGCGCAAGAGTGTTTACGGAGTATTTAAAGACTAAG
  ACATCTGATGAAATTCCAAGCTTACTCCAGTCTTATTCAAAGTCCTTGACCATATCTGCT
  CACCTTAAAAGAGATATTGTAGATAAGCGACTCCCTCTTGTGCATGACTTGGATATTAAA
  GATGGAAAGCTATCAAATTATATCGTGATGTTAGATATGTCTGTTAGTACAGCAGATGGT
  AAACAGGTAACCGTGCAATTTGTTCACGGGGTGGATGTCTACAAAGAAGCAAAGAATATT
  TTGCTTTTGTATCTCCCATATACATTTTTGGTTACAATTGCTTTTTCCTTTGTTTTTTCT
  TATTTTTATACTAAACGCTTGCTCAATCCTCTTTTTTACATTTCAGAAGTGACTAGTAAA
  ATGCAAGATTTGGATGACAATATTCGTTTTGATGAAAGTAGGAAAGATGAAGTTGGTGAA
  GTTGGAAAACAGATTAATGGTATGTATGAGCACTTGTTGAAGGTTATTCATGAGTTGGAA
  AGTCGTAATGAGCAAATTGTAAAATTGCAAAATCAAAAGGTTTCCTTTGTCCGTGGAGCA
  TCACATGAGTTGAAAACCCCTTTAGCCAGTCTTAGAATTATCCTAGAGAATATGCAGCAT
  AATATTGGAGATTACAAAGATCATCCAAAATATATTGCAAAGAGTATAAATAAGATTGAC
  CAGATGAGCCACTTATTGGAAGAAGTACTGGAGTCTTCTAAATTCCAAGAGTGGACAGAG
  TGTCGTGAGACCTTGACTGTTAAGCCAGTTTTAGTAGATATTTTATCACGTTATCAAGAA
  TTAGCTCATTCAATAGGTGTTACAATTGAAAATCAATTGACAGATGCTACCAGGGTCGTC
  ATGAGTCTTAGGGCATTGGATAAGGTTTTGACAAACCTGATTAGTAATGCAATTAAATAT
  TCAGATAAAAATGGGCGTGTAATCATATCTGAGCAAGATGGCTATCTCTCTATCAAAAAT
  ACATGTGCGCCTCTAAGTGACCAAGAACTAGAACATTTATTTGATATATTCTATCATTCT
  CAAATCGTGACAGATAAGGATGAAAGTTCCGGTTTGGGTCTTTACATTGTGAGTAATATT
  TTAGAAAGCTATCAAATGGATTATAGTTTTCTCCCTTATGAACACGGTATGGAATTTAAG
  ATTAGCTTGTAG
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1332
  

⊟Protein

⊟General

• locus tag: SPN23F_RS11430 [old locus tags: SPN23F22260 SPN23F_22260 ]
• symbol: Hk06
• description: two-component system sensor histidine kinase HK06
• length: 443
• theoretical pI: 6.28388
• theoretical MW: 50961.3
• GRAVY: -0.13544

⊟Function

• TIGRFAM:
  Signal transduction Signal transduction Two-component systems phosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 93.3)
  and 6 more
  heavy metal sensor kinase (TIGR01386; EC 2.7.13.3; HMM-score: 73.8)
  proteobacterial dedicated sortase system histidine kinase (TIGR03785; HMM-score: 59.6)
  Signal transduction Signal transduction Two-component systems TMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 33.2)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 33)
  Signal transduction Signal transduction Two-component systems putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 33)
  helix-rich protein (TIGR04523; HMM-score: 12.1)
• TheSEED: see SPN23F22260
• PFAM:
  His_Kinase_A (CL0025) HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 37.9)
  HisKA; His Kinase A (phospho-acceptor) domain (PF00512; HMM-score: 37.7)
  HAMP (CL0681) HAMP; HAMP domain (PF00672; HMM-score: 31.2)
  and 10 more
  no clan defined DUF1216; Protein of unknown function (DUF1216) (PF06746; HMM-score: 20.6)
  His_Kinase_A (CL0025) HATPase_c_5; GHKL domain (PF14501; HMM-score: 19.7)
  no clan defined LHC; Antenna complex alpha/beta subunit (PF00556; HMM-score: 17.6)
  DUF6674; Family of unknown function (DUF6674) (PF20379; HMM-score: 14.7)
  PMT2_N; Phosphoethanolamine N-methyltransferase 2 N-terminal (PF17987; HMM-score: 14.3)
  Com_YlbF; Control of competence regulator ComK, YlbF/YmcA (PF06133; HMM-score: 13.9)
  SNARE-fusion (CL0445) Syntaxin; Syntaxin (PF00804; HMM-score: 13.7)
  ArdcN (CL0700) BHD_1; Rad4 beta-hairpin domain 1 (PF10403; HMM-score: 12.4)
  no clan defined Cep57_MT_bd; Centrosome microtubule-binding domain of Cep57 (PF06657; HMM-score: 12)
  DUF1664; Protein of unknown function (DUF1664) (PF07889; HMM-score: 11.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.89
  • Cytoplasmic Membrane Score: 0.09
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 2
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.006266
  • TAT(Tat/SPI): 0.000989
  • LIPO(Sec/SPII): 0.002236
• predicted transmembrane helices (TMHMM): 2

⊟Accession numbers

• GI:
• RefSeq: WP_000594667 NCBI
• UniProt:

⊟Protein sequence

• MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGEKFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDGKLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFSYFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGKQINGMYEHLLKVIHELESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIAKSINKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIENQLTDATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQELEHLFDIFYHSQIVTDKDESSGLGLYIVSNILESYQMDYSFLPYEHGMEFKISL

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: pspC < SPN23F_RS11425 < hk06 < rr06

⊟Regulation

• regulator:

⊟Expression data

• PneumoExpress for strain D39V: SPV_2019 PneumoExpress

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Zhuo Ma, Jing-Ren Zhang
RR06 activates transcription of spr1996 and cbpA in Streptococcus pneumoniae.
J Bacteriol: 2007, 189(6);2497-509
[PubMed:17220227] [WorldCat.org] [DOI] (P p)Alistair J Standish, Uwe H Stroeher, James C Paton
The pneumococcal two-component signal transduction system RR/HK06 regulates CbpA and PspA by two distinct mechanisms.
J Bacteriol: 2007, 189(15);5591-600
[PubMed:17526693] [WorldCat.org] [DOI] (P p)Jason W Rosch, Beth Mann, Justin Thornton, Jack Sublett, Elaine Tuomanen
Convergence of regulatory networks on the pilus locus of Streptococcus pneumoniae.
Infect Immun: 2008, 76(7);3187-96
[PubMed:18443093] [WorldCat.org] [DOI] (I p)