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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 23-APR-2024

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae TIGR4
  • locus tag: SP_RS02085 [old locus tag: SP_0422 ]
  • pan locus tag?: PNEUPAN001311000
  • symbol: fabF
  • pan gene symbol?: fabF
  • synonym:
  • product: beta-ketoacyl-ACP synthase II

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SP_RS02085 [old locus tag: SP_0422 ]
  • symbol: fabF
  • product: beta-ketoacyl-ACP synthase II
  • replicon: chromosome
  • strand: +
  • coordinates: 399709..400944
  • length: 1236
  • essential: yes [1] DEG

⊟Accession numbers[edit | edit source]

  • Location: NC_003028 (399709..400944) NCBI
  • BioCyc: SP_RS02085 BioCyc
  • MicrobesOnline: see SP_0422
  • PneumoBrowse for strain D39V: SPV_0385 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAAACTAAATCGAGTAGTGGTAACAGGTTATGGAGTAACATCTCCAATCGGAAATACA
    CCAGAAGAATTTTGGAATAGTTTAGCAACTGGGAAAATCGGCATTGGTGGCATTACAAAA
    TTTGATCATAGTGACTTTGATGTGCATAATGCGGCAGAAATCCAAGATTTTCCGTTCGAT
    AAATACTTTGTAAAAAAAGATACCAACCGTTTTGATAACTATTCTTTATATGCCTTGTAT
    GCAGCCCAAGAGGCTGTAAATCATGCCAATCTTGATGTAGAGGCTCTTAATAGGGATCGT
    TTTGGTGTTATCGTTGCATCTGGTATTGGTGGAATCAAGGAAATTGAAGATCAGGTACTT
    CGCCTTCATGAAAAAGGACCCAAACGTGTCAAACCAATGACTCTTCCAAAAGCTTTACCA
    AATATGGCTTCTGGGAATGTAGCCATGCGTTTTGGTGCAAACGGTGTTTGTAAATCTATC
    AATACTGCCTGCTCTTCATCAAATGATGCGATTGGGGATGCCTTCCGCTCCATTAAGTTT
    GGTTTCCAAGATGTGATGTTGGTGGGAGGAACAGAAGCTTCTATCACACCTTTTGCCATC
    GCTGGTTTCCAAGCCTTAACAGCTCTCTCTACTACAGAGGATCCAACTCGTGCTTCGATC
    CCATTTGATAAGGATCGCAATGGGTTTGTTATGGGTGAAGGTTCAGGGATGTTGGTTCTA
    GAAAGTCTTGAACACGCTGAAAAACGTGGAGCTACTATCCTGGCTGAAGTGGTTGGTTAC
    GGAAATACTTGTGATGCCTACCACATGACTTCTCCACATCCAGAAGGTCAGGGAGCTATC
    AAGGCCATCAAACTAGCCTTGGAAGAAGCTGAGATTTCTCCAGAGCAAGTAGCCTATGTC
    AATGCTCACGGAACGTCAACTCCTGCCAATGAAAAAGGAGAAAGTGGTGCTATCGTAGCT
    GTTCTTGGTAAGGAAGTACCTGTATCATCAACCAAGTCTTTTACAGGACATTTGCTGGGG
    GCTGCGGGTGCAGTAGAAGCTATCGTCACCATCGAAGCTATGCGTCATAACTTTGTACCA
    ATGACAGCTGGGACAAGTGAAGTATCAGATTATATCGAAGCTAATGTCGTTTATGGACAA
    GGCTTGGAGAAAGAAATTCCATACGCTATTTCAAATACTTTTGGTTTTGGAGGCCACAAT
    GCAGTTCTTGCTTTCAAACGTTGGGAGAATAGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1236

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SP_RS02085 [old locus tag: SP_0422 ]
  • symbol: FabF
  • description: beta-ketoacyl-ACP synthase II
  • length: 411
  • theoretical pI: 5.43076
  • theoretical MW: 43931.3
  • GRAVY: -0.118005

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 2.3.1.179?  ExPASy
    Beta-ketoacyl-[acyl-carrier-protein] synthase II (Z)-hexadec-11-enoyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = (Z)-3-oxooctadec-13-enoyl-[acyl-carrier-protein] + CO2 + [acyl-carrier-protein]
  • ⊞TIGRFAM:
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis beta-ketoacyl-acyl-carrier-protein synthase II (TIGR03150; EC 2.3.1.179; HMM-score: 564.9)
    and 4 more
    polyketide-type polyunsaturated fatty acid synthase PfaA (TIGR02813; HMM-score: 101.5)
    Metabolism Fatty acid and phospholipid metabolism Other acetyl-CoA C-acyltransferase (TIGR01930; EC 2.3.1.16; HMM-score: 39.8)
    3-oxoadipyl-CoA thiolase (TIGR02430; EC 2.3.1.174; HMM-score: 21.3)
    Metabolism Fatty acid and phospholipid metabolism Degradation acetyl-CoA C-acyltransferase FadA (TIGR02445; EC 2.3.1.16; HMM-score: 16.3)
  • TheSEED: see SP_0422
  • ⊞PFAM:
    Thiolase (CL0046) ketoacyl-synt; Beta-ketoacyl synthase, N-terminal domain (PF00109; HMM-score: 189.8)
    and 2 more
    Ketoacyl-synt_C; Beta-ketoacyl synthase, C-terminal domain (PF02801; HMM-score: 106.9)
    Thiolase_N; Thiolase, N-terminal domain (PF00108; HMM-score: 28.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.17
    • Cytoplasmic Membrane Score: 9.51
    • Cellwall Score: 0.16
    • Extracellular Score: 0.15
    • Internal Helices: 0
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.026387
    • TAT(Tat/SPI): 0.000973
    • LIPO(Sec/SPII): 0.002855
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHSDFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFGVIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSINTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRASIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEGQGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSFTGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISNTFGFGGHNAVLAFKRWENR

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Expression data[edit | edit source]

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Jane A Thanassi, Sandra L Hartman-Neumann, Thomas J Dougherty, Brian A Dougherty, Michael J Pucci
    Identification of 113 conserved essential genes using a high-throughput gene disruption system in Streptococcus pneumoniae.
    Nucleic Acids Res: 2002, 30(14);3152-62
    [PubMed:12136097] [WorldCat.org] [DOI] (I p)    Tim van Opijnen, Andrew Camilli
    A fine scale phenotype-genotype virulence map of a bacterial pathogen.
    Genome Res: 2012, 22(12);2541-51
    [PubMed:22826510] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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