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INV200
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JJA
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MDRSPN001
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NCTC7465
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NCTC7466
serotype 2
NU83127
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SPN032672
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SPN034183
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Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 19-NOV-2015

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae A66
  • locus tag: A66_00883 [new locus tag: A66_RS04390 ]
  • pan locus tag?: PNEUPAN001963000
  • symbol: A66_00883
  • pan gene symbol?: lytB
  • synonym:
  • product: N-acetylmuramoyl-L-alanine amidase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: A66_00883 [new locus tag: A66_RS04390 ]
  • symbol: A66_00883
  • product: N-acetylmuramoyl-L-alanine amidase
  • replicon: chromosome
  • strand: +
  • coordinates: 843075..844919
  • length: 1845
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: LN847353 (843075..844919) NCBI
  • BioCyc:
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_0853 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAAGAAAGTAAGATTTATTTTTTTAGCTCTGCTATTTTTCTTAGCTAGTCCAGAGGGT
    GCAATGGCTAGTGATGGTACTTGGCAAGGAAAACAGTATCTGAAAGAAGATGGCAGTCAA
    GCAGCGAATGAGTGGGTTTTTGATACTCATTATCAATCTTGGTTCTATATAAAAGCAGAT
    GCTAACTATGCTGAAAATGAATGGCTAAAGCAAGGTGACGACTATTTTTACCTCAAATCT
    GGTGGCTATATGGCCAAATCAGAATGGGTAGAAGACAAGGGAGCCTTTTATTATCTTGAC
    CAAGATGGAAAGATGAAAAGAAATGCTTGGGTAGGAACTTCCTATGTTGGTGCAACAGGT
    GCCAAAGTAATAGAAGACTGGGTCTATGATTCTCAATACGATGCTTGGTTTTATATCAAA
    GCAGATGGACAGCACGCAGAGAAAGAATGGCTCCAAATTAAAGGGAAGGACTATTATTTC
    AAATCCGGTGGTTATCTACTGACAAGTCAGTGGATTAATCAAGCTTATGTGAATGCTAGT
    GGTGCCAAAGTACAGCAAGGTTGGCTTTTTGACAAACAATACCAATCTTGGTTTTACATC
    AAAGAAAATGGAAACTATGCTGATAAAGAATGGATTTTCGAGAATGGTCACTATTATTAT
    CTAAAATCCGGTGGCTACATGGCAGCCAATGAATGGATTTGGGATAAGGAATCTTGGTTT
    TACCTCAAGTTTGATGGCAAAATAGCTGAAAAAGAATGGGTCTACGATTATCATAGTCAA
    GCTTGGTACTACTTCAAATCCGGTGGTTACATGACAGCAAATGAATGGATTTGGGATAAG
    GAATCTTGGTTTTACCTCAAGTCTGATGGCAAAATGGCTGAAAAAGAATGGGTCTACGAT
    TCTCATAGTCAAGCCTGGTACTACTTCAAATCTGGTGGCTACATGGCGAAAAATGAGACA
    GTAGATGGTTATCAGCTTGGAAGCGATGGTAAATGGCTTGGAGGAAAAGCTACAAATGAA
    AATGCTGCTTACTATCAAGTAGTGCCTGTCACAGCCAATGTTTATGATTCAGATGGTGAA
    AAGCTTTCCTATATATCGCAAGGTAGTGTCGTATGGCTAGATAAGGATAGAAAAAGTGAT
    GACAAGCGCTTGGCTATTACTATTTCTGGTTTGTCAGGCTATATGAAAACAGAAGATTTA
    CAAGCGCTAGATGCTAGTAAGGACTTTATCCCTTATTATGAGAGTGATGGCCACCGTTTT
    TATCACTATGTGGCTCAGAATGCTAGTATCCCAGTAGCTTCTCATCTTTCTGATATGGAA
    GTAGGCAAGAAATATTATTCGGCAGATGGCCTGCATTTTGATGGTTTTAAGCTTGAGAAT
    CCCTTCCTTTTCAAAGATTTAACAGAGGCTACAAACTACAGTGCTGAAGAATTGGATAAG
    GTATTTAGTTTGCTAAACATTAACAATAGCCTTTTGGAGAACAAGGGCGCTACTTTTAAG
    GAAGCCGAAGAACATTACCATATCAATGCTCTTTATCTCCTTGCCCATAGTGCCCTAGAA
    AGTAACTGGGGAAGAAGTAAAATTGCCAAAGATAAGAATAATTTCTTTGGCATTACAGCC
    TATGATACGACCCCTTACCTTTCTGCTAAGACATTTGATGATGTGGATAAGGGAATTTTA
    GGTGCAACCAAGTGGATTAAGGAAAATTATATCGATAGGGGAAGAACTTTCCTTGGAAAC
    AAGGCTTCTGGTATGAATGTGGAATATGCTTCAGACCCTTATTGGGGCGAAAAAATTGCT
    AGTGTGATGATGAAAATCAATGAGAAGCTAGGTGGCAAAGATTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1845

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: A66_00883 [new locus tag: A66_RS04390 ]
  • symbol: A66_00883
  • description: N-acetylmuramoyl-L-alanine amidase
  • length: 614
  • theoretical pI: 5.03547
  • theoretical MW: 71100.5
  • GRAVY: -0.642345

⊟Function[edit | edit source]

  • reaction:
    EC 3.5.1.28?  ExPASy
    N-acetylmuramoyl-L-alanine amidase Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides
  • TIGRFAM:
    glucan-binding repeat (TIGR04035; HMM-score: 22.8)
    flagellar rod assembly protein/muramidase FlgJ (TIGR02541; HMM-score: 19.6)
  • TheSEED: data available for D39, Hungary19A-6, TIGR4
  • PFAM:
    Choline_binding (CL0694) Choline_bind_2; Choline-binding repeat (PF19085; HMM-score: 128.4)
    no clan defined WW_like; Peptidoglycan hydrolase LytB WW-like domain (PF17890; HMM-score: 110.8)
    and 5 more
    Choline_binding (CL0694) Choline_bind_1; Putative cell wall binding repeat (PF01473; HMM-score: 100.6)
    Lysozyme (CL0037) Glucosaminidase; Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase (PF01832; HMM-score: 69.7)
    no clan defined LytB_WW; Endo-beta-N-acetylglucosaminidase LytB WW domain (PF18342; HMM-score: 63)
    Choline_binding (CL0694) Choline_bind_3; Choline-binding repeat (PF19127; HMM-score: 36.3)
    Transthyretin (CL0287) PapC_C; PapC C-terminal domain (PF13953; HMM-score: 11.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0.01
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.18
    • Extracellular Score: 9.72
    • Internal Helices: 0
  • DeepLocPro: Extracellular
    • Cytoplasmic Score: 0.0029
    • Cytoplasmic Membrane Score: 0.0019
    • Cell wall & surface Score: 0.2376
    • Extracellular Score: 0.7576
  • SignalP: Signal peptide SP(Sec/SPI) length 23 aa
    • SP(Sec/SPI): 0.996142
    • TAT(Tat/SPI): 0.000971
    • LIPO(Sec/SPII): 0.002078
    • Cleavage Site: CS pos: 23-24. AMA-SD. Pr: 0.9843
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: CRI61594 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKKVRFIFLALLFFLASPEGAMASDGTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGYMAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKADGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFYIKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKIAEKEWVYDYHSQAWYYFKSGGYMTANEWIWDKESWFYLKSDGKMAEKEWVYDSHSQAWYYFKSGGYMAKNETVDGYQLGSDGKWLGGKATNENAAYYQVVPVTANVYDSDGEKLSYISQGSVVWLDKDRKSDDKRLAITISGLSGYMKTEDLQALDASKDFIPYYESDGHRFYHYVAQNASIPVASHLSDMEVGKKYYSADGLHFDGFKLENPFLFKDLTEATNYSAEELDKVFSLLNINNSLLENKGATFKEAEEHYHINALYLLAHSALESNWGRSKIAKDKNNFFGITAYDTTPYLSAKTFDDVDKGILGATKWIKENYIDRGRTFLGNKASGMNVEYASDPYWGEKIASVMMKINEKLGGKD

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

P García, M P González, E García, R López, J L García
LytB, a novel pneumococcal murein hydrolase essential for cell separation.
Mol Microbiol: 1999, 31(4);1275-81
[PubMed:10096093] [WorldCat.org] [DOI] (P p)Blanca De Las Rivas, José L García, Rubens López, Pedro García
Purification and polar localization of pneumococcal LytB, a putative endo-beta-N-acetylglucosaminidase: the chain-dispersing murein hydrolase.
J Bacteriol: 2002, 184(18);4988-5000
[PubMed:12193614] [WorldCat.org] [DOI] (P p)Xiao-Hui Bai, Hui-Jie Chen, Yong-Liang Jiang, Zhensong Wen, Yubin Huang, Wang Cheng, Qiong Li, Lei Qi, Jing-Ren Zhang, Yuxing Chen, Cong-Zhao Zhou
Structure of pneumococcal peptidoglycan hydrolase LytB reveals insights into the bacterial cell wall remodeling and pathogenesis.
J Biol Chem: 2014, 289(34);23403-16
[PubMed:25002590] [WorldCat.org] [DOI] (I p)

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