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serotype 1

NCBI: 17-JAN-2018

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae AP200
  • locus tag: SPAP_0997 [new locus tag: SPAP_RS04955 ]
  • pan locus tag?: PNEUPAN001963000
  • symbol: SPAP_0997
  • pan gene symbol?: lytB
  • synonym:
  • product: endo-beta-N-acetylglucosaminidase LytB

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPAP_0997 [new locus tag: SPAP_RS04955 ]
  • symbol: SPAP_0997
  • product: endo-beta-N-acetylglucosaminidase LytB
  • replicon: chromosome
  • strand: +
  • coordinates: 945100..946944
  • length: 1845
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAAGAAAGTAAGATTTATTTTTTTAGCTCTGCTATTTTTCTTAGCTAGTCCAGAGGGT
    GCAATGGCTAGTGATGGTACTTGGCAAGGAAAACAGTATCTGAAAGAAGATGGCAGTCAA
    GCAGCAAATGAGTGGGTTTTTGATACTCATTATCAATCTTGGTTCTATATAAAAGCAGAT
    GCTAACTATGCTGAAAATGAATGGCTAAAGCAAGGTGACGACTATTTTTACCTCAAATCT
    GGTGGCTATATGGCCAAATCAGAATGGGTAGAAGACAAGGGAGCCTTTTATTATCTTGAT
    CAAGATGGAAAGATGAAAAGAAATGCTTGGGTAGGAACTTCCTATGTTGGTGCAACAGGT
    GCCAAAGTAATAGAAGACTGGGTCTATGATTCTCAATACGATGCTTGGTTTTATATCAAA
    GCAGATGGACAGCACGCAGAGAAAGAATGGCTCCAAATTAAAGGGAAGGACTATTATTTC
    AAATCCGGTGGTTATCTACTGACAAGTCAGTGGATTAATCAAGCTTATGTGAATGCTAGT
    GGTGCCAAAGTACAGCAAGGTTGGCTTTTTGACAAACAATACCAATCTTGGTTTTACATC
    AAAGAAAATGGAAACTATGCTGATAAAGAATGGATTTTCGAGAATGGTCACTATTATTAT
    CTAAAATCCGGTGGCTACATGGCAGCCAATGAATGGATTTGGGATAAGGAATCTTGGTTT
    TATCTCAAATTTGATGGGAAAATGGCTGAAAAAGAATGGGTCTACGATTCTCATAGTCAA
    GCTTGGTACTACTTCAAATCCGGTGGTTACATGACAGCCAATGAATGGATTTGGGATAAG
    GAATCTTGGTTTTACCTCAAGTCTGATGGTAAAATGGCTGAAAAAGAATGGGTCTACGAT
    TCTCATAGTCAAGCCTGGTACTACTTCAAATCTGGTGGCTACATGGCGAAAAATGAGACA
    GTAGATGGTTATCAGCTTGGAAGCGATGGTAAATGGCTTGGAGGAAAAACTACAAATGAA
    AATGCTGCTTACTATCAAGTAGTGCCTGTTACAGCCAATGTTTATGATTCAGATGGTGAA
    AAGCTTTCCTATATATCGCAAGGTAGTGTCGTATGGCTAGATAAGGATAGAAAAAGTGAT
    GACAAGCGCTTGGCTATTACTATTTCTGGTTTGTCAGGCTATATGAAAACAGAAGATTTA
    CAAGCGCTAGATGCTAGTAAGGACTTTATCCCTTATTATGAGAGTGATGGCCACCGTTTT
    TATCACTATGTGGCTCAGAATGCTAGTATCCCAGTAGCTTCTCATCTTTCTGATATGGAA
    GTAGGCAAGAAATATTATTCGGCAGATGGCCTGCATTTTGATGGTTTTAAGCTTGAGAAT
    CCCTTCCTTTTCAAAGATTTAACAGAGGCTACAAACTACAGTGCTGAAGAATTGGATAAG
    GTATTTAGTTTGCTAAACATTAACAATAGCCTTTTGGAGAACAAGGGCGCTACTTTTAAG
    GAAGCCGAAGAACATTACCATATCAATGCTCTTTATCTCCTTGCCCATAGTGCCCTAGAA
    AGTAACTGGGGAAGAAGTAAAATTGCCAAAGATAAGAATAATTTCTTTGGCATTACAGCC
    TATGATACGACCCCTTACCTTTCTGCTAAGACATTTGATGATGTGGATAAGGGAATTTTA
    GGTGCAACCAAGTGGATTAAGGAAAATTATATCGATAGGGGAAGAACTTTCCTTGGAAAC
    AAGGCTTCTGGTATGAATGTGGAATATGCTTCAGACCCTTATTGGGGCGAAAAAATTGCT
    AGTGTGATGATGAAAATCAATGAGAAGCTAGGTGGCAAAGATTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1845

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPAP_0997 [new locus tag: SPAP_RS04955 ]
  • symbol: SPAP_0997
  • description: endo-beta-N-acetylglucosaminidase LytB
  • length: 614
  • theoretical pI: 5.03547
  • theoretical MW: 71072.5
  • GRAVY: -0.649837

⊟Function[edit | edit source]

  • TIGRFAM:
    glucan-binding repeat (TIGR04035; HMM-score: 22.1)
    flagellar rod assembly protein/muramidase FlgJ (TIGR02541; HMM-score: 19.7)
  • TheSEED  :
    • Endo-beta-N-acetylglucosaminidase (EC 3.2.1.96)
  • PFAM:
    Choline_binding (CL0694) Choline_bind_2; Choline-binding repeat (PF19085; HMM-score: 133.4)
    no clan defined WW_like; Peptidoglycan hydrolase LytB WW-like domain (PF17890; HMM-score: 110.8)
    and 4 more
    Choline_binding (CL0694) Choline_bind_1; Putative cell wall binding repeat (PF01473; HMM-score: 105.9)
    Lysozyme (CL0037) Glucosaminidase; Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase (PF01832; HMM-score: 69.7)
    no clan defined LytB_WW; Endo-beta-N-acetylglucosaminidase LytB WW domain (PF18342; HMM-score: 63)
    Choline_binding (CL0694) Choline_bind_3; Choline-binding repeat (PF19127; HMM-score: 44.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0.01
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.18
    • Extracellular Score: 9.72
    • Internal Helices: 0
  • DeepLocPro: Extracellular
    • Cytoplasmic Score: 0.003
    • Cytoplasmic Membrane Score: 0.0024
    • Cell wall & surface Score: 0.2303
    • Extracellular Score: 0.7643
  • SignalP: Signal peptide SP(Sec/SPI) length 23 aa
    • SP(Sec/SPI): 0.996142
    • TAT(Tat/SPI): 0.000971
    • LIPO(Sec/SPII): 0.002078
    • Cleavage Site: CS pos: 23-24. AMA-SD. Pr: 0.9843
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: ADM84586 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKKVRFIFLALLFFLASPEGAMASDGTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGYMAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKADGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFYIKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKMAEKEWVYDSHSQAWYYFKSGGYMTANEWIWDKESWFYLKSDGKMAEKEWVYDSHSQAWYYFKSGGYMAKNETVDGYQLGSDGKWLGGKTTNENAAYYQVVPVTANVYDSDGEKLSYISQGSVVWLDKDRKSDDKRLAITISGLSGYMKTEDLQALDASKDFIPYYESDGHRFYHYVAQNASIPVASHLSDMEVGKKYYSADGLHFDGFKLENPFLFKDLTEATNYSAEELDKVFSLLNINNSLLENKGATFKEAEEHYHINALYLLAHSALESNWGRSKIAKDKNNFFGITAYDTTPYLSAKTFDDVDKGILGATKWIKENYIDRGRTFLGNKASGMNVEYASDPYWGEKIASVMMKINEKLGGKD

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription[edit | edit source]

  • transcription start site:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

P García, M P González, E García, R López, J L García
LytB, a novel pneumococcal murein hydrolase essential for cell separation.
Mol Microbiol: 1999, 31(4);1275-81
[PubMed:10096093] [WorldCat.org] [DOI] (P p)Blanca De Las Rivas, José L García, Rubens López, Pedro García
Purification and polar localization of pneumococcal LytB, a putative endo-beta-N-acetylglucosaminidase: the chain-dispersing murein hydrolase.
J Bacteriol: 2002, 184(18);4988-5000
[PubMed:12193614] [WorldCat.org] [DOI] (P p)Xiao-Hui Bai, Hui-Jie Chen, Yong-Liang Jiang, Zhensong Wen, Yubin Huang, Wang Cheng, Qiong Li, Lei Qi, Jing-Ren Zhang, Yuxing Chen, Cong-Zhao Zhou
Structure of pneumococcal peptidoglycan hydrolase LytB reveals insights into the bacterial cell wall remodeling and pathogenesis.
J Biol Chem: 2014, 289(34);23403-16
[PubMed:25002590] [WorldCat.org] [DOI] (I p)

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