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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 02-FEB-2021

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae D39
  • locus tag: SPD_RS10670 [old locus tag: SPD_2017 ]
  • pan locus tag?: PNEUPAN003841000
  • symbol: cbpA
  • pan gene symbol?: pspC
  • synonyms: pbcA, spsA
  • product: choline-binding protein CbpA

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPD_RS10670 [old locus tag: SPD_2017 ]
  • symbol: cbpA
  • product: choline-binding protein CbpA
  • replicon: chromosome
  • strand: -
  • coordinates: 1995045..1997150
  • length: 2106
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: NC_008533 (1995045..1997150) NCBI
  • BioCyc: G1G6V-2181 BioCyc
  • MicrobesOnline: see SPD_2017
  • PneumoBrowse for strain D39V: SPV_2017 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTTTGCATCAAAAAGCGAAAGAAAAGTACATTATTCAATTCGTAAATTTAGTATTGGA
    GTAGCTAGTGTAGCTGTTGCCAGTCTTGTTATGGGAAGTGTGGTTCATGCGACAGAGAAC
    GAGGGAAGTACCCAAGCAGCCACTTCTTCTAATATGGCAAAGACAGAACATAGGAAAGCT
    GCTAAACAAGTCGTCGATGAATATATAGAAAAAATGTTGAGGGAGATTCAACTAGATAGA
    AGAAAACATACCCAAAATGTCGCCTTAAACATAAAGTTGAGCGCAATTAAAACGAAGTAT
    TTGCGTGAATTAAATGTTTTAGAAGAGAAGTCGAAAGATGAGTTGCCGTCAGAAATAAAA
    GCAAAGTTAGACGCAGCTTTTGAGAAGTTTAAAAAAGATACATTGAAACCAGGAGAAAAG
    GTAGCAGAAGCTAAGAAGAAGGTTGAAGAAGCTAAGAAAAAAGCCGAGGATCAAAAAGAA
    GAAGATCGTCGTAACTACCCAACCAATACTTACAAAACGCTTGAACTTGAAATTGCTGAG
    TTCGATGTGAAAGTTAAAGAAGCGGAGCTTGAACTAGTAAAAGAGGAAGCTAAAGAATCT
    CGAAACGAGGGCACAATTAAGCAAGCAAAAGAGAAAGTTGAGAGTAAAAAAGCTGAGGCT
    ACAAGGTTAGAAAACATCAAGACAGATCGTAAAAAAGCAGAAGAAGAAGCTAAACGAAAA
    GCAGATGCTAAGTTGAAGGAAGCTAATGTAGCGACTTCAGATCAAGGTAAACCAAAGGGG
    CGGGCAAAACGAGGAGTTCCTGGAGAGCTAGCAACACCTGATAAAAAAGAAAATGATGCG
    AAGTCTTCAGATTCTAGCGTAGGTGAAGAAACTCTTCCAAGCTCATCCCTGAAATCAGGA
    AAAAAGGTAGCAGAAGCTGAGAAGAAGGTTGAAGAAGCTGAGAAAAAAGCCAAGGATCAA
    AAAGAAGAAGATCGCCGTAACTACCCAACCAATACTTACAAAACGCTTGACCTTGAAATT
    GCTGAGTCCGATGTGAAAGTTAAAGAAGCGGAGCTTGAACTAGTAAAAGAGGAAGCTAAG
    GAACCTCGAGACGAGGAAAAAATTAAGCAAGCAAAAGCGAAAGTTGAGAGTAAAAAAGCT
    GAGGCTACAAGGTTAGAAAACATCAAGACAGATCGTAAAAAAGCAGAAGAAGAAGCTAAA
    CGAAAAGCAGCAGAAGAAGATAAAGTTAAAGAAAAACCAGCTGAACAACCACAACCAGCG
    CCGGCTACTCAACCAGAAAAACCAGCTCCAAAACCAGAGAAGCCAGCTGAACAACCAAAA
    GCAGAAAAAACAGATGATCAACAAGCTGAAGAAGACTATGCTCGTAGATCAGAAGAAGAA
    TATAATCGCTTGACTCAACAGCAACCGCCAAAAACTGAAAAACCAGCACAACCATCTACT
    CCAAAAACAGGCTGGAAACAAGAAAACGGTATGTGGTACTTCTACAATACTGATGGTTCA
    ATGGCAACAGGATGGCTCCAAAACAACGGTTCATGGTACTATCTAAACGCTAATGGTGCT
    ATGGCGACAGGATGGCTCCAAAACAATGGTTCATGGTACTATCTAAACGCTAATGGTTCA
    ATGGCAACAGGATGGCTCCAAAACAATGGTTCATGGTACTACCTAAACGCTAATGGTGCT
    ATGGCGACAGGATGGCTCCAATACAATGGTTCATGGTACTACCTAAACAGCAATGGCGCT
    ATGGCGACAGGATGGCTCCAATACAATGGCTCATGGTACTACCTCAACGCTAATGGTGAT
    ATGGCGACAGGATGGCTCCAAAACAACGGTTCATGGTACTACCTCAACGCTAATGGTGAT
    ATGGCGACAGGATGGCTCCAATACAACGGTTCATGGTATTACCTCAACGCTAATGGTGAT
    ATGGCGACAGGTTGGGTGAAAGATGGAGATACCTGGTACTATCTTGAAGCATCAGGTGCT
    ATGAAAGCAAGCCAATGGTTCAAAGTATCAGATAAATGGTACTATGTCAATGGCTCAGGT
    GCCCTTGCAGTCAACACAACTGTAGATGGCTATGGAGTCAATGCCAATGGTGAATGGGTA
    AACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2106

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPD_RS10670 [old locus tag: SPD_2017 ]
  • symbol: CbpA
  • description: choline-binding protein CbpA
  • length: 701
  • theoretical pI: 8.27203
  • theoretical MW: 79097.5
  • GRAVY: -1.08787

⊟Function[edit | edit source]

  • TIGRFAM:
    glucan-binding repeat (TIGR04035; HMM-score: 63)
    gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 53.5)
  • TheSEED: see SPD_2017
  • PFAM:
    Choline_binding (CL0694) Choline_bind_3; Choline-binding repeat (PF19127; HMM-score: 205.7)
    Choline_bind_1; Putative cell wall binding repeat (PF01473; HMM-score: 196.6)
    and 6 more
    no clan defined RICH; RICH domain (PF05062; HMM-score: 101.9)
    Choline_binding (CL0694) Choline_bind_2; Choline-binding repeat (PF19085; HMM-score: 47.4)
    no clan defined YSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 42.3)
    DUF4224; Domain of unknown function (DUF4224) (PF13986; HMM-score: 13.4)
    VirE1; Single-strand DNA-binding protein (PF12189; HMM-score: 12.6)
    tRNA_synt_II (CL0040) DUF366; Domain of unknown function (DUF366) (PF04017; HMM-score: 6.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0.01
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.18
    • Extracellular Score: 9.72
    • Internal Helix: 1
  • SignalP: Signal peptide SP(Sec/SPI) length 37 aa
    • SP(Sec/SPI): 0.953942
    • TAT(Tat/SPI): 0.035984
    • LIPO(Sec/SPII): 0.003031
    • Cleavage Site: CS pos: 37-38. VHA-TE. Pr: 0.9028
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MFASKSERKVHYSIRKFSIGVASVAVASLVMGSVVHATENEGSTQAATSSNMAKTEHRKAAKQVVDEYIEKMLREIQLDRRKHTQNVALNIKLSAIKTKYLRELNVLEEKSKDELPSEIKAKLDAAFEKFKKDTLKPGEKVAEAKKKVEEAKKKAEDQKEEDRRNYPTNTYKTLELEIAEFDVKVKEAELELVKEEAKESRNEGTIKQAKEKVESKKAEATRLENIKTDRKKAEEEAKRKADAKLKEANVATSDQGKPKGRAKRGVPGELATPDKKENDAKSSDSSVGEETLPSSSLKSGKKVAEAEKKVEEAEKKAKDQKEEDRRNYPTNTYKTLDLEIAESDVKVKEAELELVKEEAKEPRDEEKIKQAKAKVESKKAEATRLENIKTDRKKAEEEAKRKAAEEDKVKEKPAEQPQPAPATQPEKPAPKPEKPAEQPKAEKTDDQQAEEDYARRSEEEYNRLTQQQPPKTEKPAQPSTPKTGWKQENGMWYFYNTDGSMATGWLQNNGSWYYLNANGAMATGWLQNNGSWYYLNANGSMATGWLQNNGSWYYLNANGAMATGWLQYNGSWYYLNSNGAMATGWLQYNGSWYYLNANGDMATGWLQNNGSWYYLNANGDMATGWLQYNGSWYYLNANGDMATGWVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNGSGALAVNTTVDGYGVNANGEWVN

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

S Hammerschmidt, G Bethe, P H Remane, G S Chhatwal
Identification of pneumococcal surface protein A as a lactoferrin-binding protein of Streptococcus pneumoniae.
Infect Immun: 1999, 67(4);1683-7
[PubMed:10085004] [WorldCat.org] [DOI] (P p)S Hammerschmidt, M P Tillig, S Wolff, J P Vaerman, G S Chhatwal
Species-specific binding of human secretory component to SpsA protein of Streptococcus pneumoniae via a hexapeptide motif.
Mol Microbiol: 2000, 36(3);726-36
[PubMed:10844660] [WorldCat.org] [DOI] (P p)Hanna Jarva, Robert Janulczyk, Jens Hellwage, Peter F Zipfel, Lars Björck, Seppo Meri
Streptococcus pneumoniae evades complement attack and opsonophagocytosis by expressing the pspC locus-encoded Hic protein that binds to short consensus repeats 8-11 of factor H.
J Immunol: 2002, 168(4);1886-94
[PubMed:11823523] [WorldCat.org] [DOI] (P p)Francesco Iannelli, Marco R Oggioni, Gianni Pozzi
Allelic variation in the highly polymorphic locus pspC of Streptococcus pneumoniae.
Gene: 2002, 284(1-2);63-71
[PubMed:11891047] [WorldCat.org] [DOI] (P p)Sandhya Dave, Stephanie Carmicle, Sven Hammerschmidt, Michael K Pangburn, Larry S McDaniel
Dual roles of PspC, a surface protein of Streptococcus pneumoniae, in binding human secretory IgA and factor H.
J Immunol: 2004, 173(1);471-7
[PubMed:15210807] [WorldCat.org] [DOI] (P p)Christine Elm, Manfred Rohde, Jean-Pierre Vaerman, Gursharan S Chhatwal, Sven Hammerschmidt
Characterization of the interaction of the pneumococcal surface protein SpsA with the human polymeric immunoglobulin receptor (hpIgR).
Indian J Med Res: 2004, 119 Suppl;61-5
[PubMed:15232164] [WorldCat.org] (P p)Sven Hammerschmidt, Vaibhav Agarwal, Anja Kunert, Steffi Haelbich, Christine Skerka, Peter F Zipfel
The host immune regulator factor H interacts via two contact sites with the PspC protein of Streptococcus pneumoniae and mediates adhesion to host epithelial cells.
J Immunol: 2007, 178(9);5848-58
[PubMed:17442969] [WorldCat.org] [DOI] (P p)Antoine Dieudonné-Vatran, Stefanie Krentz, Anna M Blom, Seppo Meri, Birgitta Henriques-Normark, Kristian Riesbeck, Barbara Albiger
Clinical isolates of Streptococcus pneumoniae bind the complement inhibitor C4b-binding protein in a PspC allele-dependent fashion.
J Immunol: 2009, 182(12);7865-77
[PubMed:19494311] [WorldCat.org] [DOI] (I p)Tauseef M Asmat, Katharina Klingbeil, Inga Jensch, Gerhard Burchhardt, Sven Hammerschmidt
Heterologous expression of pneumococcal virulence factor PspC on the surface of Lactococcus lactis confers adhesive properties.
Microbiology (Reading): 2012, 158(Pt 3);771-780
[PubMed:22222496] [WorldCat.org] [DOI] (I p)Sylvia Voss, Teresia Hallström, Malek Saleh, Gerhard Burchhardt, Thomas Pribyl, Birendra Singh, Kristian Riesbeck, Peter F Zipfel, Sven Hammerschmidt
The choline-binding protein PspC of Streptococcus pneumoniae interacts with the C-terminal heparin-binding domain of vitronectin.
J Biol Chem: 2013, 288(22);15614-27
[PubMed:23603906] [WorldCat.org] [DOI] (I p)Ulrike Binsker, Thomas P Kohler, Krystin Krauel, Sylvia Kohler, Hansjörg Schwertz, Sven Hammerschmidt
Pneumococcal Adhesins PavB and PspC Are Important for the Interplay with Human Thrombospondin-1.
J Biol Chem: 2015, 290(23);14542-55
[PubMed:25897078] [WorldCat.org] [DOI] (I p)Shanshan Du, Cláudia Vilhena, Samantha King, Alfredo Sahagún-Ruiz, Sven Hammerschmidt, Christine Skerka, Peter F Zipfel
Molecular analyses identifies new domains and structural differences among Streptococcus pneumoniae immune evasion proteins PspC and Hic.
Sci Rep: 2021, 11(1);1701
[PubMed:33462258] [WorldCat.org] [DOI] (I e)S Hammerschmidt, S R Talay, P Brandtzaeg, G S Chhatwal
SpsA, a novel pneumococcal surface protein with specific binding to secretory immunoglobulin A and secretory component.
Mol Microbiol: 1997, 25(6);1113-24
[PubMed:9350867] [WorldCat.org] [DOI] (P p)

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