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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 27-FEB-2015

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae SPNA45
  • locus tag: SPNA45_00270 [new locus tag: SPNA45_RS14355 ]
  • pan locus tag?: PNEUPAN003528000
  • symbol: SPNA45_00270
  • pan gene symbol?: lytA
  • synonym:
  • product: Thiol-activated cytolysin

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPNA45_00270 [new locus tag: SPNA45_RS14355 ]
  • symbol: SPNA45_00270
  • product: Thiol-activated cytolysin
  • replicon: chromosome
  • strand: +
  • coordinates: 284123..285502
  • length: 1380
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: HE983624 (284123..285502) NCBI
  • BioCyc:
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_1737 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGCAGGTAGGACCTGTTGATAATGGTGCCTGGGACGTTGGGGGCGGTTGGAATGCTGAG
    ACCTATGCAGCGGTTGAACTGATTGAAAGCCATTCAACTAAAGAAGAGTTCATGACGGAC
    TACCGCCTTTATATCGAACTCTTACGCAATCTAGCAGATGAAGCAGGTTTGCCGAAAACG
    CTTGATACAGGGAGTTTAGCTGGAATTAAAACGCACGAGTATTGCACGAATAACCAACCA
    AACAACCACTCAGACCATGTGGATCCATACCCTTACTTGGCAAAATGGGGCATTAGCCGT
    GAGCAGTTTAAGCATGATATTGAGAACGGCTTGACGATTGAAACAGGCTGGCAGAAGAAT
    GACACTGGCTACTGGTACGTACATTCAGACGGCTCTTATCCAAAAGACAAGTTTGAAAAA
    ATCACGGCTCACAGCATGGAACAACTCAAGGTCAAGTTTGGTTCTGACTTTGAAAAGACA
    GGGAATTCTCTTGATATTGATTTTAACTCTGTCCATTCAGGCGAAAAGCAGATTCAGATT
    GTTAATTTTAAGCAGATTTATTATACAGTCAGCGTAGATGCTGTTAAAAATCCAGGAGAT
    GTGTTTCAAGATACTGTAACGGTAGAGGATTTAAAACAGAGAGGAATTTCTGCAGAGCGT
    CCTTTGGTCTATATTTCGAGTGTTGCTTATGGGCGCCAAGTCTATCTCAAGTTGGAAACC
    ACGAGTAAGAGTGATGAAGTAGAGGCTGCTTTTGAAGCTTTGATAAAAGGAGTCAAGGTA
    GCTCCTCAGACAGAGTGGAAGCAGATTTTGGACAATACAGAAGTGAAGGCGGTTATTTTA
    GGGGGCGACCCAAGTTCGGGTGCCCGAGTTGTAACAGGCAAGGTGGATATGGTAGAGGAC
    TTGATTCAAGAAGGCAGTCGCTTTACAGCAGATCATCCAGGCTTGCCGATTTCCTATACA
    ACTTCTTTTTTACGTGACAATGTAGTTGCGACCTTTCAAAACAGTACAGACTATGTTGAG
    ACTAAGGTTACAGCTTACAGAAACGGAGATTTACTGCTGGATCATAGTGGTGCCTATGTT
    GCCCAATATTATATTACTTGGGATGAATTATCCTATGATCATCAAGGTAAGGAAGTCTTG
    ACTCCTAAGGCTTGGGACAGAAATGGGCAGGATTTGACGGCTCACTTTACCACTAGTATT
    CCTTTAAAAGGGAATGTTCGTAATCTCTCTGTCAAAATTAGAGAGTGTACCGGGCTTGCC
    TGGGAATGGTGGCGTACGGTTTATGAAAAAACCGATTTGCCACTAGTGCGTAAGCGGACG
    ATTTCTATTTGGGGAACAACTCTCTATCCTCAGGTAGAAGATAAGGTAGAAAATGACTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPNA45_00270 [new locus tag: SPNA45_RS14355 ]
  • symbol: SPNA45_00270
  • description: Thiol-activated cytolysin
  • length: 459
  • theoretical pI: 4.81187
  • theoretical MW: 51855.4
  • GRAVY: -0.536601

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED: data available for D39, Hungary19A-6, TIGR4
  • PFAM:
    CDC (CL0293) Thiol_cytolysin; Thiol-activated cytolysin (PF01289; HMM-score: 325.8)
    and 2 more
    no clan defined Thiol_cytolys_C; Thiol-activated cytolysin beta sandwich domain (PF17440; HMM-score: 168.7)
    Effector_1; Effector from type III secretion system (PF04518; HMM-score: 15.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0.1
    • Cytoplasmic Membrane Score: 0.14
    • Cellwall Score: 0.15
    • Extracellular Score: 9.6
    • Internal Helices: 0
  • DeepLocPro: Extracellular
    • Cytoplasmic Score: 0.0005
    • Cytoplasmic Membrane Score: 0.0014
    • Cell wall & surface Score: 0.0002
    • Extracellular Score: 0.9979
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005672
    • TAT(Tat/SPI): 0.000221
    • LIPO(Sec/SPII): 0.000604
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: CCM07560 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDYRLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGISREQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKITAHSMEQLKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTVTVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQTEWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTSFLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWDELSYDHQGKEVLTPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRKRTISIWGTTLYPQVEDKVEND

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription[edit | edit source]

  • transcription start site:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

P García, M Paz González, E García, J L García, R López
The molecular characterization of the first autolytic lysozyme of Streptococcus pneumoniae reveals evolutionary mobile domains.
Mol Microbiol: 1999, 33(1);128-38
[PubMed:10411730] [WorldCat.org] [DOI] (P p)P Balachandran, S K Hollingshead, J C Paton, D E Briles
The autolytic enzyme LytA of Streptococcus pneumoniae is not responsible for releasing pneumolysin.
J Bacteriol: 2001, 183(10);3108-16
[PubMed:11325939] [WorldCat.org] [DOI] (P p)E Díaz, R López, J L García
Role of the major pneumococcal autolysin in the atypical response of a clinical isolate of Streptococcus pneumoniae.
J Bacteriol: 1992, 174(17);5508-15
[PubMed:1355082] [WorldCat.org] [DOI] (P p)Peter Mellroth, Robert Daniels, Alice Eberhardt, Daniel Rönnlund, Hans Blom, Jerker Widengren, Staffan Normark, Birgitta Henriques-Normark
LytA, major autolysin of Streptococcus pneumoniae, requires access to nascent peptidoglycan.
J Biol Chem: 2012, 287(14);11018-29
[PubMed:22334685] [WorldCat.org] [DOI] (I p)J V Höltje, A Tomasz
Specific recognition of choline residues in the cell wall teichoic acid by the N-acetylmuramyl-L-alanine amidase of Pneumococcus.
J Biol Chem: 1975, 250(15);6072-6
[PubMed:238995] [WorldCat.org] (P p)A M Berry, R A Lock, D Hansman, J C Paton
Contribution of autolysin to virulence of Streptococcus pneumoniae.
Infect Immun: 1989, 57(8);2324-30
[PubMed:2568343] [WorldCat.org] [DOI] (P p)J F Garcia-Bustos, A Tomasz
Teichoic acid-containing muropeptides from Streptococcus pneumoniae as substrates for the pneumococcal autolysin.
J Bacteriol: 1987, 169(2);447-53
[PubMed:2879828] [WorldCat.org] [DOI] (P p)A Tomasz, P Moreillon, G Pozzi
Insertional inactivation of the major autolysin gene of Streptococcus pneumoniae.
J Bacteriol: 1988, 170(12);5931-4
[PubMed:2903859] [WorldCat.org] [DOI] (P p)L V Howard, H Gooder
Specificity of the autolysin of Streptococcus (Diplococcus) pneumoniae.
J Bacteriol: 1974, 117(2);796-804
[PubMed:4149515] [WorldCat.org] [DOI] (P p)P Usobiaga, F J Medrano, M Gasset, J L Garciá, J L Saiz, G Rivas, J Laynez, M Menéndez
Structural organization of the major autolysin from Streptococcus pneumoniae.
J Biol Chem: 1996, 271(12);6832-8
[PubMed:8636107] [WorldCat.org] [DOI] (P p)

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