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serotype 4
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D39V
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Hungary19A-6
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EF3030
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670-6B
serotype 6B
6A-10
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70585
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A026
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A66
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AP200
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CGSP14
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HU-OH
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Hu15
serotype 19A
Hu17
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INV104
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INV200
serotype 14
JJA
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MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
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SPN034156
serotype 3
SPN034183
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SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
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TCH8431/19A
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Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 26-AUG-2017

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae OXC141
  • locus tag: SPNOXC08670 [new locus tag: SPNOXC_RS04705 ]
  • pan locus tag?: PNEUPAN001963000
  • symbol: lytB
  • pan gene symbol?: lytB
  • synonym:
  • product: putative endo-beta-N-acetylglucosaminidase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPNOXC08670 [new locus tag: SPNOXC_RS04705 ]
  • symbol: lytB
  • product: putative endo-beta-N-acetylglucosaminidase
  • replicon: chromosome
  • strand: +
  • coordinates: 877016..878992
  • length: 1977
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAAGAAAGTAAGATTTATTTTTTTAGCTCTGCTATTTTTCTTAGCTAGTCCAGAGGGT
    GCAATGGCTAGTGATGGTACTTGGCAAGGAAAACAGTATCTGAAAGAAGATGGCAGTCAA
    GCAGCGAATGAGTGGGTTTTTGATACTCATTATCAATCTTGGTTCTATATAAAAGCAGAT
    GCTAACTATGCTGAAAATGAATGGCTAAAGCAAGGTGACGACTATTTTTACCTCAAATCT
    GGTGGCTATATGGCCAAATCAGAATGGGTAGAAGACAAGGGAGCCTTTTATTATCTTGAC
    CAAGATGGAAAGATGAAACGAAATGCTTGGGTAGGAACTTCCTATGTTGGTGCAACAGGT
    GCCAAAGTAATAGAAGACTGGGTCTATGATTCTCAATACGATGCTTGGTTTTATATCAAA
    GCAGATGGACAGCACGCAGAGAAAGAATGGCTCCAAATTAAAGGGAAGGACTATTATTTC
    AAATCCGGTGGTTATCTACTGACAAGTCAGTGGATTAATCAAGCTTATGTGAATGCTAGT
    GGTGCCAAAGTACAGCAAGGTTGGCTTTTTGACAAACAATACCAATCTTGGTTTTACATC
    AAAGAAAATGGAAACTATGCTGATAAAGAATGGATTTTCGAGAATGGTCACTATTATTAT
    CTAAAATCTGGTGGCTACATGGCAGCCAATGAATGGATTTGGGATAAGGAATCTTGGTTT
    TATCTCAAATTTGATGGGAAAATAGCTGAAAAAGAATGGGTCTACGATTCTCATAGTCAA
    GCTTGGTACTACTTCAAATCCGGTGGCTACATGGCAGCCAATGAATGGATTTGGGATAAG
    GAATCTTGGTTTTACCTCAAGTTTGATGGGAAAATAGCTGAAAAAGAATGGGTCTACGAT
    TCTCATAGTCAAGCCTGGTACTACTTCAAATCCGGTGGTTACATGACAGCCAATGAATGG
    ATTTGGGATAAGGAATCTTGGTTTTACCTCAAGTCTGATGGCAAAATGGCTGAAAAAGAA
    TGGGTCTACGATTCTCATAGTCAAGCTTGGTACTACTTCAAATCTGGTGGCTACATGGCG
    AAAAATGAGACAGTAGATGGTTATCAGCTTGAAAGCGATGGTAAATGGCTTGGAGGAAAA
    GCTACAAATGAAAATGCTGCTTACTATCAAGTAGTGCCTGTTACAGCCAATGTTTATGAT
    TCAGATGGTGAAAAGCTTTCCTATATATCGCAAGGTAGTGTCGTATGGCTAGATAAGGAT
    AGAAAAAGTGATGACAAGCGCTTGGCTATTACTATTTCTGGTTTGTCAGGCTATATGAAA
    ACAGAAGATTTACAAGCGCTAGATGCTAGTAAGGACTTTATCCCTTATTATGAGAGTGAT
    GGCCACCGTTTTTATCACTATGTGGCTCAGAATGCTAGTATCCCAGTAGCTTCTCATCTT
    TCTGATATGGAAGTAGGCAAGAAATATTATTCGGCAGATGGCCTGCATTTTGATGGTTTT
    AAGCTTGAGAATCCCTTCCTTTTCAAAGATTTAACAGAGGCTACAAACTACAGTGCTGAA
    GAATTGGATAAGGTATTTAGTTTGCTAAACATTAACAATAGCCTTTTGGAGAACAAGGGC
    GCTACTTTTAAGGAAGCCGAAGAACATTACCATATCAATGCCCTTTATCTCCTTGCCCAT
    AGTGCCCTAGAAAGTAACTGGGGAAGAAGTAAAATTGCCAAAGATAAGAATAATTTCTTT
    GGCATTACAGCCTATGATACGACCCCTTACCTTTCTGCCAAGACATTTGATGATGTGGAT
    AAGGGAATTTTAGGTGCAACCAAGTGGATTAAGGAAAATTATATCGATAGGGGAAGAACT
    TTCCTTGGAAACAAGGCTTCTGGTATGAATGTGGAATATGCTTCAGACCCTTATTGGGGC
    GAAAAAATTGCTAGTGTGATGATGAAAATCAATGAGAAGCTAGGTGGCAAAGATTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1977

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPNOXC08670 [new locus tag: SPNOXC_RS04705 ]
  • symbol: LytB
  • description: putative endo-beta-N-acetylglucosaminidase
  • length: 658
  • theoretical pI: 4.96689
  • theoretical MW: 76540.5
  • GRAVY: -0.656687

⊟Function[edit | edit source]

  • TIGRFAM:
    glucan-binding repeat (TIGR04035; HMM-score: 22.2)
    flagellar rod assembly protein/muramidase FlgJ (TIGR02541; HMM-score: 19.4)
  • TheSEED  :
    • Endo-beta-N-acetylglucosaminidase (EC 3.2.1.96)
  • PFAM:
    Choline_binding (CL0694) Choline_bind_2; Choline-binding repeat (PF19085; HMM-score: 142.3)
    Choline_bind_1; Putative cell wall binding repeat (PF01473; HMM-score: 125.5)
    and 5 more
    no clan defined WW_like; Peptidoglycan hydrolase LytB WW-like domain (PF17890; HMM-score: 110.7)
    Lysozyme (CL0037) Glucosaminidase; Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase (PF01832; HMM-score: 71.2)
    no clan defined LytB_WW; Endo-beta-N-acetylglucosaminidase LytB WW domain (PF18342; HMM-score: 62.7)
    Choline_binding (CL0694) Choline_bind_3; Choline-binding repeat (PF19127; HMM-score: 49.4)
    RRM (CL0221) Nup35_RRM_2; Nup53/35/40-type RNA recognition motif (PF14605; HMM-score: 11.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0.01
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.18
    • Extracellular Score: 9.72
    • Internal Helices: 0
  • DeepLocPro: Extracellular
    • Cytoplasmic Score: 0.0081
    • Cytoplasmic Membrane Score: 0.003
    • Cell wall & surface Score: 0.2598
    • Extracellular Score: 0.7292
  • SignalP: Signal peptide SP(Sec/SPI) length 23 aa
    • SP(Sec/SPI): 0.996142
    • TAT(Tat/SPI): 0.000971
    • LIPO(Sec/SPII): 0.002078
    • Cleavage Site: CS pos: 23-24. AMA-SD. Pr: 0.9843
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: CBW32524 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKKVRFIFLALLFFLASPEGAMASDGTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGYMAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKADGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFYIKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKIAEKEWVYDSHSQAWYYFKSGGYMAANEWIWDKESWFYLKFDGKIAEKEWVYDSHSQAWYYFKSGGYMTANEWIWDKESWFYLKSDGKMAEKEWVYDSHSQAWYYFKSGGYMAKNETVDGYQLESDGKWLGGKATNENAAYYQVVPVTANVYDSDGEKLSYISQGSVVWLDKDRKSDDKRLAITISGLSGYMKTEDLQALDASKDFIPYYESDGHRFYHYVAQNASIPVASHLSDMEVGKKYYSADGLHFDGFKLENPFLFKDLTEATNYSAEELDKVFSLLNINNSLLENKGATFKEAEEHYHINALYLLAHSALESNWGRSKIAKDKNNFFGITAYDTTPYLSAKTFDDVDKGILGATKWIKENYIDRGRTFLGNKASGMNVEYASDPYWGEKIASVMMKINEKLGGKD

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

P García, M P González, E García, R López, J L García
LytB, a novel pneumococcal murein hydrolase essential for cell separation.
Mol Microbiol: 1999, 31(4);1275-81
[PubMed:10096093] [WorldCat.org] [DOI] (P p)Blanca De Las Rivas, José L García, Rubens López, Pedro García
Purification and polar localization of pneumococcal LytB, a putative endo-beta-N-acetylglucosaminidase: the chain-dispersing murein hydrolase.
J Bacteriol: 2002, 184(18);4988-5000
[PubMed:12193614] [WorldCat.org] [DOI] (P p)Xiao-Hui Bai, Hui-Jie Chen, Yong-Liang Jiang, Zhensong Wen, Yubin Huang, Wang Cheng, Qiong Li, Lei Qi, Jing-Ren Zhang, Yuxing Chen, Cong-Zhao Zhou
Structure of pneumococcal peptidoglycan hydrolase LytB reveals insights into the bacterial cell wall remodeling and pathogenesis.
J Biol Chem: 2014, 289(34);23403-16
[PubMed:25002590] [WorldCat.org] [DOI] (I p)

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