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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 19-NOV-2015

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae A66
  • locus tag: A66_01038 [new locus tag: A66_RS05195 ]
  • pan locus tag?: PNEUPAN002295000
  • symbol: A66_01038
  • pan gene symbol?: zmpA
  • synonym: iga
  • product: IgA1 protease

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: A66_01038 [new locus tag: A66_RS05195 ]
  • symbol: A66_01038
  • product: IgA1 protease
  • replicon: chromosome
  • strand: +
  • coordinates: 997874..1001797
  • length: 3924
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: LN847353 (997874..1001797) NCBI
  • BioCyc:
  • MicrobesOnline:
  • PneumoBrowse for strain D39V:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAAAAGTATTTTGGTGAAAAACAAGAGCGTTTTTCATTTAGAAAATTATCAGTAGGA
    CTTGTATCTGCAACGATTTCAAGTTTATTTTTTATGTCTGTATTAGCTAGTTCATCTGTG
    GATGCTCAAGAAACTGCGGGAGTTCACTATAAATATGTGGCAGATTCAGAGCTATCATCA
    GAAGAAAAGAAGCAGCTTGTCTATGATATTCCGACATACGTGGAGAATGATGATGAAACT
    TATTATCTTGTTTATAAGTTAAATTCTCAAAATCAACTGGCGGAATTACCAAATACTGGA
    AGCAAGAATGAGAGGCAAGCCCTAGTTGCTGGTGCTAGCTTAGCTGCTCTGGGAATTTTA
    ATTTTTGCTGTTTCCAAGAAAAAGGTTAAGAATAAAACGGTATTACATTTAGTATTGGTT
    GCGGGAATAGGAAATGGTGTCTTAGTTTCAGTCCATGCTTTAGAAAATCATCTTTTGCTA
    AATTACAATACGGACTATGAATTGACCTCTGGAGAAAAATTACCTCTTCCTAAAGAGATT
    TCAGGTTACACTTATATTGGATATATCAAAGAGGGAAAAACGACTTCTGATTTTGAAGTA
    AGTAATCAAGAAAAATCAGCAGCCACTCCTACAAAACAACAAAAGGTGGATTATAATGTT
    ACACCAAATTTTGTAGACCATCCATCAACAGTACAAGCTATTCAGGAACAAACACCTGTT
    TCTTCAACTAAGCCGACAGAAGTTCAAGTAGTTGAAAAACCTTTCTCTACTGAATTAATC
    AATCCAAGAAAAGAAGAGAAACAATCTTCAGATTCTCAAGAACAATTAGCCGAACATAAG
    AATCTAGAAACGAAGAAAGAGGAGAAGATTTCTCCAAAAGAAAAGACTGGGGTAAATACA
    TTAAATCCACAGGATGAAGTTTTATCAGGTCAATTGAACAAACCTGAACTCTTATATCGT
    GAGGAAACTATAGAGACAAAAATAGATTTTCAAGAAGAAATTCAAGAAAATCCTGATTTA
    GCTGAAGGAACTGTAAGAGTAAAACAAGAAGGTAAATTAGGTAAGAAAGTTGAAATCGTC
    AGAATATTCTCTGTAAACAAGGAAGAAGTTTCGCGAGAAATTGTTTCAACTTCAACGACT
    GCGCCTATTCCAAGAATAGTCGAAAAAGGTACTAAAAAAACTCAAGTTATAAAGGAACAA
    CCTGAGACTGGTGTAGAACATAAGGACGTACAGTCTGGAGCTATTGTTGAACCCGCAATT
    CAGCCTGAGTTGCCAGAAGCAGTAGTAAGTGATAAAGGTGAACCAGAGCAGGTAGCCCCA
    TTGCCAGAATATAAGGGTAATATTGAGCAAGTAAAACCTGAAACTCCGGTTGAGAAGACC
    AAAGAACAAGGTCCAGAAAAAACTGAAGAAGTTCCAATAAAACCAACAGAAGAAACACCA
    GTAAATCCAAATGAAGGTACTACAGAAGGAACCTCAATTCAAGAAGCAGAAAATCCAGTT
    CAACCTGCAGAAGAATCAACAACGAATTCAGAGAAAGTATCACCAGATACATCTAGCGAA
    AATACTGGGGAAGTGTCCAGTAATCCTAGTGATTCGACAACCTCAGTTGGAGAATCAAAT
    AAACCAGAACATAATGACTCTAAAAATGAAAATTCAGAAAAAACTGGAGAAGAAGTTCCA
    GTAAATCCAAATGAAGGCACAGTAGAAGGTACCTCAAATCAAGAAACAGAAACACCAGTT
    CAACCTGCAGAAGAAACACAAACAAACTCTGGGAAAATAGCTAACGAAAATACTGGAGAA
    GTATCCAATAAACCTAGTGATTCAAAACCACCAGTTGAAGAATCAAATCAACCAGAAAAT
    TCAGGTAATACAACATCAGAGAATGGACAAACAGAACCAGAACCATCAAACGGAAATTCA
    ACTGAGGATGTTTCAACCGAATCAAACACATACAATTCAAATGGAAATGAAGAAATTAAA
    CAAGAAAATGAACTAGACCCTGATAAAAAGGTAGAAGAACCAGAGAAAACACTTGAATTA
    AGAAATGTGTCAGAAATTGAATTGTACAGTGTGACAGATAACGTGTATAAACAGCAAGTG
    TTTTTAGAGAAGCTTCCAAGTAATATCGATAGATTCTTTGTAAAAGTAAAATCGACCACC
    ATTAAAGATGTCTATCTACCAGTAGAATCAATTGTGGAAGAAACTAAAGATGGTAAGCAA
    GTTTATAAAATCACAGCAAAAGTAGAGAAGCTTCAGCAGGAGATAGAAAGTAGATATAAA
    GATAATTTTACCTTCTATCTAGCTAAGAAGGGAACAGAAGAAACAACAAACTTTACTTCC
    TTTAGTAATCTGGTCAAAGCTATAAACCAAAATCTCTCTGGAACCTATCATTTAGGAGCT
    AGCCTGAATGCTAACGAAGTGGAGCTTAGTACTGATGATAAATCCTATATCAAGGGAACC
    TTTACTGGTCAGTTGATTGGTGAAAAAGATGGTAAGCATTATGCTATTTACAACTTGAAA
    AAGCCTCTTTTTGAAAGCTTGAGAGGGGCCACAATAGAAAAGTTGAGTCTGAAAAATGTA
    TCTATTTCAGGGAAAGATGATATCGGTTCACTGGCAAATGAAGCTAAAAATGGAACAACC
    ATTAATGAAGTTCATGTTGACGGTCTCTTAGGAGGTGAACGTGGTATTGGTGGTTTAGTG
    GCTAAAGTGGATCAATCCAATATCACAAATAGTAGTTTTACTGGAAGAATTGTTAATACT
    TATGAAACAACTGCTTCCTATAATATCGGCGGCCTGGTCGGTCATTTGACAGGAAGCAAA
    GCTTCACTGACTAAGTCAAAAGCGACAGTGGTCATTTCATCAAATACAAATAGTTCTGAC
    CAGACCGTTGGAGGTATCGCTGGTCTTGTAGACAAAGATGCGCATATTCAGAATAGCTAT
    TCTGAAGGGGATATTAATAATAGCCAACGTTTTGGAAAAGTTGCTGGTATAGCAGGTAAC
    TTATGGGATCGTGAATCAAATAGTGAAAATCATGCTGGAAGATTGACTAATGTGCTTAGT
    GGTGTCAATGTAACTAATGGAAATGCCATTAGTAGTTACCATTATAATGGAATGAAAATT
    ACTGATGCATTTAGTAATAAAGCGAATAAAGTTTTCAATGTCACTTTAGAGAAGGATGAG
    GTCGTCAGCAAAGAATCCTTTGAGGAAAGAGGAACAATGCTAGATGCTTCTCAAATTGCA
    AGCAAAAAAGCAGAAATCAATCTTCTCACTCCACCAATAGTGGAACCCCTCTCAACAAGC
    GGTAAGAAAGACAGTGATTTTTCTAAGATAGCCCATTATCAAGCCAACCGTGCTTTGGTT
    TATAAGAACATTGAAAAATTGTTACCTTTCTATAACAAGGCGACCATCGTGAAATACGGA
    AACCTGGTTAAGGAGAACAGTATCTTATATCAAAAAGAACTCTTGTCTGCAGTTATGATG
    AAGGATGACCAAGTAATCACAGATATTATTTCCAACAAACAGACTGCAAACAAACTCTTG
    CTTCACTACAAGGACCATTCATCTGAAAAGTTTGATCTCAGGTACCAGGATGATTTTGCC
    AATTTGGCAGAATATAGCATTGGAGATAGTGGGCTTCTCTATACGCCAAATCAATTCTTG
    TATGACCAAGACTCTATCATCAATCAAGTTTTACCTGAATTACAGCAGGTTGCCTACGAT
    TCAGAAGCTATCAGAAAGACACTCGGAATTTCTCCAGAGGTCAAGCAAACTGAGCTCTAT
    ATGGAAGACCAGTTTACCAAAACAAAGCAAGATTTGGCAAACAGTTTGAAAAAACTCTTG
    TCTGCAGATGCTGGACTTGCTGGTGACAACCCAGTTACCAGAGGCTATCTTAACGTTGGT
    ATAACTTTAGCTATGGTCAGGTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2760
    2820
    2880
    2940
    3000
    3060
    3120
    3180
    3240
    3300
    3360
    3420
    3480
    3540
    3600
    3660
    3720
    3780
    3840
    3900
    3924

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: A66_01038 [new locus tag: A66_RS05195 ]
  • symbol: A66_01038
  • description: IgA1 protease
  • length: 1307
  • theoretical pI: 4.62297
  • theoretical MW: 143866
  • GRAVY: -0.685616

⊟Function[edit | edit source]

  • reaction:
    EC 3.4.24.13?  ExPASy
    IgA-specific metalloendopeptidase Cleavage of Pro-|-Thr bond in the hinge region of the heavy chain of human IgA
  • TIGRFAM:
    gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 45)
    and 1 more
    Cell structure Cell envelope Other LPXTG cell wall anchor domain (TIGR01167; HMM-score: 21.2)
  • TheSEED: data available for D39, TIGR4
  • PFAM:
    no clan defined Peptidase_M26_N; M26 IgA1-specific Metallo-endopeptidase N-terminal region (PF05342; HMM-score: 280.7)
    and 5 more
    Peptidase_M26_C; M26 IgA1-specific Metallo-endopeptidase C-terminal region (PF07580; HMM-score: 89.9)
    G5 (CL0593) G5; G5 domain (PF07501; HMM-score: 56.2)
    no clan defined Glug; The GLUG motif (PF07581; HMM-score: 46.3)
    YSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 37.6)
    Gram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 30.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 10
    • Extracellular Score: 0
    • Internal Helices: 2
  • DeepLocPro: Cell wall & surface
    • Cytoplasmic Score: 0.0002
    • Cytoplasmic Membrane Score: 0.0028
    • Cell wall & surface Score: 0.9155
    • Extracellular Score: 0.0815
  • SignalP: Signal peptide SP(Sec/SPI) length 42 aa
    • SP(Sec/SPI): 0.91723
    • TAT(Tat/SPI): 0.067255
    • LIPO(Sec/SPII): 0.005174
    • Cleavage Site: CS pos: 42-43. VDA-QE. Pr: 0.9143
  • predicted transmembrane helices (TMHMM): 3

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: CRI61749 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQETAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSKNERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGIGNGVLVSVHALENHLLLNYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDYNVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQLAEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYREETIETKIDFQEEIQENPDLAEGTVRVKQEGKLGKKVEIVRIFSVNKEEVSREIVSTSTTAPIPRIVEKGTKKTQVIKEQPETGVEHKDVQSGAIVEPAIQPELPEAVVSDKGEPEQVAPLPEYKGNIEQVKPETPVEKTKEQGPEKTEEVPIKPTEETPVNPNEGTTEGTSIQEAENPVQPAEESTTNSEKVSPDTSSENTGEVSSNPSDSTTSVGESNKPEHNDSKNENSEKTGEEVPVNPNEGTVEGTSNQETETPVQPAEETQTNSGKIANENTGEVSNKPSDSKPPVEESNQPENSGNTTSENGQTEPEPSNGNSTEDVSTESNTYNSNGNEEIKQENELDPDKKVEEPEKTLELRNVSEIELYSVTDNVYKQQVFLEKLPSNIDRFFVKVKSTTIKDVYLPVESIVEETKDGKQVYKITAKVEKLQQEIESRYKDNFTFYLAKKGTEETTNFTSFSNLVKAINQNLSGTYHLGASLNANEVELSTDDKSYIKGTFTGQLIGEKDGKHYAIYNLKKPLFESLRGATIEKLSLKNVSISGKDDIGSLANEAKNGTTINEVHVDGLLGGERGIGGLVAKVDQSNITNSSFTGRIVNTYETTASYNIGGLVGHLTGSKASLTKSKATVVISSNTNSSDQTVGGIAGLVDKDAHIQNSYSEGDINNSQRFGKVAGIAGNLWDRESNSENHAGRLTNVLSGVNVTNGNAISSYHYNGMKITDAFSNKANKVFNVTLEKDEVVSKESFEERGTMLDASQIASKKAEINLLTPPIVEPLSTSGKKDSDFSKIAHYQANRALVYKNIEKLLPFYNKATIVKYGNLVKENSILYQKELLSAVMMKDDQVITDIISNKQTANKLLLHYKDHSSEKFDLRYQDDFANLAEYSIGDSGLLYTPNQFLYDQDSIINQVLPELQQVAYDSEAIRKTLGISPEVKQTELYMEDQFTKTKQDLANSLKKLLSADAGLAGDNPVTRGYLNVGITLAMVR

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription[edit | edit source]

  • transcription start site:

⊟Expression data[edit | edit source]

  • PneumoExpress for strain D39V:

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Jeffrey N Weiser, Deborah Bae, Claudine Fasching, Ronald W Scamurra, Adam J Ratner, Edward N Janoff
Antibody-enhanced pneumococcal adherence requires IgA1 protease.
Proc Natl Acad Sci U S A: 2003, 100(7);4215-20
[PubMed:12642661] [WorldCat.org] [DOI] (P p)Damiana Chiavolini, Guido Memmi, Tiziana Maggi, Francesco Iannelli, Gianni Pozzi, Marco R Oggioni
The three extra-cellular zinc metalloproteinases of Streptococcus pneumoniae have a different impact on virulence in mice.
BMC Microbiol: 2003, 3;14
[PubMed:12841855] [WorldCat.org] [DOI] (I e)E N Janoff, J B Rubins, C Fasching, D Charboneau, J T Rahkola, A G Plaut, J N Weiser
Pneumococcal IgA1 protease subverts specific protection by human IgA1.
Mucosal Immunol: 2014, 7(2);249-56
[PubMed:23820749] [WorldCat.org] [DOI] (I p)H Lomholt
Evidence of recombination and an antigenically diverse immunoglobulin A1 protease among strains of Streptococcus pneumoniae.
Infect Immun: 1995, 63(11);4238-43
[PubMed:7591053] [WorldCat.org] [DOI] (P p)K Poulsen, J Reinholdt, M Kilian
Characterization of the Streptococcus pneumoniae immunoglobulin A1 protease gene (iga) and its translation product.
Infect Immun: 1996, 64(10);3957-66
[PubMed:8926055] [WorldCat.org] [DOI] (P p)J H Wani, J V Gilbert, A G Plaut, J N Weiser
Identification, cloning, and sequencing of the immunoglobulin A1 protease gene of Streptococcus pneumoniae.
Infect Immun: 1996, 64(10);3967-74
[PubMed:8926056] [WorldCat.org] [DOI] (P p)

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