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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 31-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae TIGR4
  • locus tag: SP_1072 [new locus tag: SP_RS05300 ]
  • pan locus tag?: PNEUPAN002210000
  • symbol: dnaG
  • pan gene symbol?: dnaG
  • synonym:
  • product: DNA primase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SP_1072 [new locus tag: SP_RS05300 ]
  • symbol: dnaG
  • product: DNA primase
  • replicon: chromosome
  • strand: +
  • coordinates: 1008420..1010180
  • length: 1761
  • essential: yes [1] other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGTTGACAAACAAGTCATTGAAGAAATCAAAAACAATGCCAACATTGTGGAAGTCATA
    GGAGATGTGATTTCTTTACAAAAGGCAGGACGGAACTATCTAGGGCTCTGTCCTTTTCAT
    GGTGAAAAAACACCATCTTTCAATGTTGTAGAGGACAAGCAGTTTTATCACTGTTTCGGT
    TGTGGTCGCTCAGGTGATGTCTTTAAGTTCATCGAGGAGTACCAAGGGGTTCCCTTTATA
    GAAGCAGTCCAAATCTTAGGTCAGCGTGTTGGAATTGAGGTTGAAAAACCGCTTTATAGT
    GAGCAGAAGTCAGCCTCACCTCACCAAGCTCTTTATGATATGCACGAAGATGCAGCCAAA
    TTTTACCATGCTATTCTCATGACAACGACTATGGGCGAAGAGGCCAGAAATTACCTTTAT
    CAGCGGGGGTTGACAGATGAAGTGCTTAAACATTTTTGGATTGGTTTAGCACCTCCAGAA
    CGAAACTATCTCTATCAACGTTTGTCTGATCAGTATCGTGAAGAGGATTTACTGGATTCA
    GGCCTGTTTTATCTCTCGGATGCCAATCAATTTGTAGACACCTTTCACAATCGCATTATG
    TTTCCCCTGACAAATGACCAAGGAAAGGTCATTGCCTTCTCAGGTCGTATCTGGCAAAAA
    ACGGATTCACAAACTTCTAAGTATAAAAACAGCCGATCGACTGCAATTTTTAACAAAAGT
    TACGAATTATATCATATGGATAGGGCAAAAAGATCTTCTGGAAAAGCTAGTGAGATTTAC
    CTGATGGAAGGATTCATGGATGTTATTGCAGCCTATCGGGCTGGAATCGAAAATGCTGTG
    GCGTCGATGGGAACAGCCTTGAGTCGAGAGCATGTTGAGCATCTGAAAAGGTTAACCAAG
    AAATTGGTTCTTGTTTACGATGGAGATAAGGCTGGGCAAGCCGCGACATTGAAAGCATTG
    GATGAAATTGGTGATATGCCTGTGCAAATCGTCAGCATGCCTGATAACTTGGATCCTGAT
    GAGTATCTACAAAAAAATGGTCCAGAAGACTTGGCCTATCTATTAACGAAAACTCGTATT
    AGTCCGATTGAGTTCTACATTCATCAGTACAAACCTGAAAACAGTGAAAATCTGCAGGCT
    CAGATTGAGTTTCTTGAAAAAATAGCTCCCTTGATTGTTCAAGAAAAGTCCATCGCTGCT
    CAAAACAGCTATATTCATATTTTAGCTGACAGTCTGGCGTCCTTTGATTATACCCAGATT
    GAGCAGATTGTTAATGAGAGTCGTCAGGTGCAAAGGCAGAATCGCATGGAAGGAATTTCC
    AGACCGACGCCAATCACCATGCCTGTCACCAAGCAGTTATCGGCTATTATGAGGGCAGAA
    GCCCATCTACTCTATCGGATGATGGAATCCCCTCTTGTTTTGAACGATTACCGTTTGCGA
    GAAGACTTTGCATTTGCTACACCTGAATTTCAGGTCTTATATGACTTGCTTGGCCAGTAT
    GGAAATCTTCCTCCAGAAGTTTTAGCAGAGCAGACAGAGGAAGTTGAAAGAGCTTGGTAC
    CAAGTTTTAGCTCAGGATTTGCCTGCTGAGATATCGCCGCAGGAACTTAGTGAAGTAGAG
    ATGACTCGAAACAAGGCTCTCTTGAATCAGGACAATATGAGAATCAAAAAGAAGGTGCAG
    GAAGCTAGCCATGTAGGAGATACAGATACAGCCCTAGAAGAATTGGAACGTTTAATTTCC
    CAAAAGAGAAGAATGGAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1761

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SP_1072 [new locus tag: SP_RS05300 ]
  • symbol: DnaG
  • description: DNA primase
  • length: 586
  • theoretical pI: 4.95206
  • theoretical MW: 67255.8
  • GRAVY: -0.454437

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.7.-?  ExPASy
  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA primase (TIGR01391; EC 2.7.7.-; HMM-score: 455)
    and 1 more
    Unknown function General DNA primase-like protein (TIGR00646; HMM-score: 28.5)
  • TheSEED  :
    • DNA primase (EC 2.7.7.-)
    DNA Metabolism DNA replication DNA-replication  DNA primase (EC 2.7.7.-)
  • PFAM:
    Zn_Beta_Ribbon (CL0167) zf-CHC2; CHC2 zinc finger (PF01807; HMM-score: 124.8)
    no clan defined Toprim_N; DNA primase catalytic core, N-terminal domain (PF08275; HMM-score: 120.6)
    and 4 more
    Toprim-like (CL0413) Toprim_4; Toprim domain (PF13662; HMM-score: 67.7)
    Toprim_2; Toprim-like (PF13155; HMM-score: 61.5)
    Toprim; Toprim domain (PF01751; HMM-score: 27.6)
    Toprim_3; Toprim domain (PF13362; HMM-score: 26.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9922
    • Cytoplasmic Membrane Score: 0.0049
    • Cell wall & surface Score: 0.001
    • Extracellular Score: 0.0019
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.028188
    • TAT(Tat/SPI): 0.000906
    • LIPO(Sec/SPII): 0.001948
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKTPSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVPFIEAVQILGQRVGIEVEKPLYSEQKSASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPERNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIWQKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKRSSGKASEIYLMEGFMDVIAAYRAGIENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAATLKALDEIGDMPVQIVSMPDNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFLEKIAPLIVQEKSIAAQNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMEGISRPTPITMPVTKQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVLAEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVGDTDTALEELERLISQKRRME

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription[edit | edit source]

  • transcription start site: 1008390 [2]

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Tim van Opijnen, Andrew Camilli
    A fine scale phenotype-genotype virulence map of a bacterial pathogen.
    Genome Res: 2012, 22(12);2541-51
    [PubMed:22826510] [WorldCat.org] [DOI] (I p)
  2. ↑ 2.0 2.1 2.2 Indu Warrier, Nikhil Ram-Mohan, Zeyu Zhu, Ariana Hazery, Haley Echlin, Jason Rosch, Michelle M Meyer, Tim van Opijnen
    The Transcriptional landscape of Streptococcus pneumoniae TIGR4 reveals a complex operon architecture and abundant riboregulation critical for growth and virulence.
    PLoS Pathog: 2018, 14(12);e1007461
    [PubMed:30517198] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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