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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 31-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae TIGR4
  • locus tag: SP_1075 [new locus tag: SP_RS05315 ]
  • pan locus tag?: PNEUPAN002213000
  • symbol: cpoA
  • pan gene symbol?: lafB
  • synonym:
  • product: glycosyl transferase CpoA

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SP_1075 [new locus tag: SP_RS05315 ]
  • symbol: cpoA
  • product: glycosyl transferase CpoA
  • replicon: chromosome
  • strand: +
  • coordinates: 1011777..1012793
  • length: 1017
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGTTGAGTTCAAGTGAGAAAGTAGCAGGACAGGGAGTTTCAGGTGCTTACCGTGAATTA
    GTTCGTCTTCTTCACCGTGCTGCCAAGGACCAATTGATTGTTACAGAAAATCTTCCAATC
    GAGGCAGATGTGACTCACTTTCATACGATTGATTTTCCCTATTATTTATCAACCTTCCAA
    AAGAAACGCTCAGGGAGAAAGATTGGCTATGTGCATTTCTTGCCAGCTACACTTGAGGGA
    AGTTTGAAAATTCCATTTTTCTTAAAGGGAATTGTGAAACGCTATGTATTTTCTTTTTAC
    AACCGGATGGAGCACTTGGTTGTGGTCAATCCTATGTTTATTGAGGATTTGGTAGCAGCT
    GGTATTCCACGTGAAAAAGTGACCTATATTCCTAACTTTGTCAACAAGGAAAAATGGCAT
    CCTCTACCACAAGAAGAGGTAGTCAGACTGCGCACAGATCTTGGTCTTAGTGACAATCAG
    TTTATCGTAGTAGGTGCTGGGCAAGTTCAGAAACGTAAAGGGATTGATGACTTTATCCGT
    CTGGCTGAGGAATTGCCTCAGATTACCTTTATCTGGGCTGGTGGCTTCTCTTTTGGTGGT
    ATGACAGATGGTTATGAACACTATAAGAAAATTATGGAAAATCCCCCTAAAAATTTGATT
    TTTCCAGGCATTGTATCGCCAGAGCGGATGCGCGAATTGTATGCTCTAGCGGATCTTTTC
    TTGTTGCCTAGTTACAATGAGCTCTTTCCTATGACTATTTTAGAAGCTGCGAGTTGTGAG
    GCTCCTATTATGTTGCGTGATTTAGATCTCTATAAGGTGATTTTGGAGGGAAATTATCGG
    GCGACAGCGGGTAGAGAAGAGATGAAAGAGGCTATTTTGGAATATCAAGCAAATCCTGCT
    GTCTTAAAAGATCTCAAAGAAAAGGCTAAGAATATTTCCAGAGAGTATTCTGAAGAGCAT
    CTGTTACAAATCTGGTTGGACTTTTATGAGAAACAAGCCGCTTTAGGGAGAAAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1017

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SP_1075 [new locus tag: SP_RS05315 ]
  • symbol: CpoA
  • description: glycosyl transferase CpoA
  • length: 338
  • theoretical pI: 7.29177
  • theoretical MW: 38825.7
  • GRAVY: -0.187278

⊟Function[edit | edit source]

  • TIGRFAM:
    Unknown function Enzymes of unknown specificity glycosyltransferase, MSMEG_0565 family (TIGR04047; HMM-score: 59.9)
    and 8 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs N-acetyl-alpha-D-glucosaminyl L-malate synthase BshA (TIGR03999; EC 2.4.1.-; HMM-score: 45.2)
    glycosyltransferase, GG-Bacteroidales peptide system (TIGR04157; EC 2.4.1.-; HMM-score: 42.3)
    sugar transferase, PEP-CTERM/EpsH1 system associated (TIGR03088; HMM-score: 37.4)
    PEP-CTERM/exosortase A-associated glycosyltransferase, Daro_2409 family (TIGR04063; EC 2.4.-.-; HMM-score: 35.3)
    colanic acid biosynthesis glycosyltransferase WcaI (TIGR04007; EC 2.4.-.-; HMM-score: 33.8)
    D-inositol-3-phosphate glycosyltransferase (TIGR03449; EC 2.4.1.250; HMM-score: 33.6)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides glycogen synthase, Corynebacterium family (TIGR02149; HMM-score: 29.8)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides glycogen/starch synthase, ADP-glucose type (TIGR02095; EC 2.4.1.21; HMM-score: 12.2)
  • TheSEED  :
    • Glycosyltransferase LafB, responsible for the formation of Gal-Glc-DAG
    Cell Wall and Capsule Gram-Positive cell wall components Polyglycerolphosphate lipoteichoic acid biosynthesis  Glycosyltransferase LafB, responsible for the formation of Gal-Glc-DAG
  • PFAM:
    GT-B (CL0113) Glycos_transf_1; Glycosyl transferases group 1 (PF00534; HMM-score: 52.2)
    Glyco_trans_1_4; Glycosyl transferases group 1 (PF13692; HMM-score: 44.3)
    and 3 more
    Glyco_transf_4; Glycosyltransferase Family 4 (PF13439; HMM-score: 30.8)
    Glyco_trans_1_2; Glycosyl transferases group 1 (PF13524; HMM-score: 17.9)
    Glyco_trans_4_4; Glycosyl transferase 4-like domain (PF13579; HMM-score: 15.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.0678
    • Cytoplasmic Membrane Score: 0.9005
    • Cell wall & surface Score: 0.0005
    • Extracellular Score: 0.0313
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004978
    • TAT(Tat/SPI): 0.001029
    • LIPO(Sec/SPII): 0.001518
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVTENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVKRYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLRTDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYKKIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRDLDLYKVILEGNYRATAGREEMKEAILEYQANPAVLKDLKEKAKNISREYSEEHLLQIWLDFYEKQAALGRK

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription[edit | edit source]

  • transcription start sites: 1011750, 1011536 [1]

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 1.2 Indu Warrier, Nikhil Ram-Mohan, Zeyu Zhu, Ariana Hazery, Haley Echlin, Jason Rosch, Michelle M Meyer, Tim van Opijnen
    The Transcriptional landscape of Streptococcus pneumoniae TIGR4 reveals a complex operon architecture and abundant riboregulation critical for growth and virulence.
    PLoS Pathog: 2018, 14(12);e1007461
    [PubMed:30517198] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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