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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 07-FEB-2021

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae AP200
  • locus tag: SPAP_RS03230 [old locus tag: SPAP_0653 ]
  • pan locus tag?: PNEUPAN001606000
  • symbol: SPAP_RS03230
  • pan gene symbol?: zmpB
  • synonym:
  • product: G5 domain-containing protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPAP_RS03230 [old locus tag: SPAP_0653 ]
  • symbol: SPAP_RS03230
  • product: G5 domain-containing protein
  • replicon: chromosome
  • strand: +
  • coordinates: 623931..629594
  • length: 5664
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: NC_014494 (623931..629594) NCBI
  • BioCyc: SPAP_RS03230 BioCyc
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_0577 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
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    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    GTGTTTAAAAAAGACCGTTTTTCAATTCGTAAGATTAAGGGAGTTGTAGGCTCTGTATTT
    CTTGGAAGCCTTTTGATGGCTCCTTCTGTAGTGGACGCAGCCACCTATCACTATGTAAAT
    AAAGAGATTATTTCACAAGAAGCTAAAGATTTAATTCAGACAGGAAAGCCTGACAGGAAT
    GAAGTTGTATATGGTTTGGTGTATCAAAAAGATCAGTTGCCTCAAACAGGGACAGAAGCA
    TCTGTTTTGACAGCTTTTGGTTTGCTGACTGTTGGGAGCTTGCTTTTAATCTACAAGAGA
    AAGAAAATTGCTAGCGTCTTTCTAGTTGGAACTATGGGATTGGTAGTTCTTCCTAGTGCA
    GGGGCTGTAGACCCAGTTGCGACCCTAGCACTGGCTAGTCGAGAGGGTGTTGTTGAAATG
    GAGGGCTATCGCTATGTTGGTTATCTATCAGGTGACATCCTCAAAACGCTTGGCTTGGAC
    ACTGTTTTAGAAGAAACCTCAGCAAAACCTGGAGAGGTGACTGTAGTCGAAGTTGAGACT
    CCTCAATCAACAACAAATCAGGAGCAAGCTAGGACAGAAAACCAAGTAGTAGAGACAGAG
    GAAGCTCCAAAAGAAGAAGCACCTAAAACAGAAGAAAGTCCAAAGGAAGAACCAAAATCG
    GAGGTAAAACCTACTGACGACACCCTTCCTAAAGTAGAAGAGGGGAAAGAAGATTCAGCA
    GAACCAGCTCCAGTTGAAGAAGTAGGTGGAGAAGTTGAGTCAAAACCAGAGGAAAAAGTA
    GCAGTTAAGCCAGAAAGTCAACCATCAGACAAACCAGCTGAGGAATCAAAAGTTGAACAA
    GCAGGTGAACCAGTCGCGCCAAGAAAAGACGAACAGGCACCAGTCGAGCCAGAAAATCAA
    CCAGAAGCTCCTGAAGAAGAGAAGGCTGTAGAGGAAACACCGAAACAAGAAGAGTCAACT
    CCAGATACCAAGGCTGAAGAAACTGTAGAACCAAAAGAGGAGACTAAAACAGCTAAGGGG
    ACGCAAGAAGAAGATTACGTGGGTGAGGCTCTTGTTCCAGAACAATTACCTGAGTATAAG
    GTGACTGAAGGAACACTCGTTGAAGAATCAACTACAGAACTTGACTATAAAACTGAAACG
    ACTGAGGACCCAACCAAGTACACGGACGAAGAAACTGTCCTACGAAATGGTGAAAAAGGA
    AGTCAAGTTACAAAAACAACCTATAAAACAATAGGAGGGGTTAAAACTGACCAAGTTCTT
    TCAACCAGTACGGAAGTCACCAAGGAGCCTGTAAATCAACAGGTAATCCGTGGTACAAAA
    CCAATTGAAGGGACAACGGTTGAGACAAGAGAAGAAATCATTCCTTTTGAAACAAAAGAA
    CAAGAAGATGACACTTTAAAACGCGGAACAAGACAAGTAACTCAAGAAGGTGTAAACGAT
    AAGAAACAAATTACTGAAACCTATAAAACAATTCGCGGAGAAAAAACAAGTGACGCTCCT
    ACTATCACAGAAACAGTGATTGAAAAACCTCAAGACAAAATTATTAAGAATGGCACAAAA
    GAGTTAGAGAAACCAACCTTGGAATGGGCTAAAACAGATAAAGATGTCTTGAAGAAGAGT
    GCGACAGCTAGTTATACATTGACTAAACCAGCTGGAGTAGAAATTAAATCGATTAAAGTA
    GCTCTAAAAGATAATACTGGAACAGTTGTTAAAGAAGTAACGGTAGAAGAAAATAACTTA
    CAAGCTACTTTAGACAACTTGAAATATTATCAAGGTTATACACTATCCACAACCATGGTC
    TATGATCGAGGTAACGGTGAAGAAACTGAAATATTAGAAGATAAAGAAGTTCAGTTAGAC
    CTTAAAAAAGTTGAGATTAAAGATATTAAAGAAACAAGATTGATGAAAGTGGATGAGAAT
    GGAAATGAAACAGATAGTAGCCTTCTAGAAACTGTTCCAGAAAATCTAACGTCATATTAT
    CTAAAAATTACCACTAATCATAATAAAGTAACAAGGCTTGCCATTACTAATATAGAGGAA
    GTAACTGAGGAGGAGACATCCTTATTTAAAGTTACTGCTTCAGCTCCTGATTTAATCCAA
    CGTGATTCAAATAATAGTTTGAGTCAAACATACACTTATTACATAGAGAAACCCAAAGCA
    TCGCAAAATAACGTTTACTATAACTTCAAAGATTTAGTAGATGCTATGCAGAAAAATCCT
    AACGGTGAGTTTAAATTAGGATCGGATTTAAATGCAACAAATGTACCTACACCAAGTAAA
    TCATATGTAACAGGTAAATTTACAGGTCACTTAACAAGTGTTGATGGTAAGCATTTCTCC
    ATTCATAACACTGCTCGTCCATTATTTGATAGAGTTGAAGGGGGTTCTATCAAAAATATT
    AATTTAATTAACGTTAATATTAATATGCCTTGGGCAAATCAATTAGCTTCATTAGCTCAA
    ACAGTGAAAAATGCAACTGTTGAAAATATTAAAGTAACGGGTACTATCGTTGGTAATAAC
    AATGTTGCGGGTATTTTGGATAAATTAGATGATGCAGGTATTATGACTAATGTTGCTTTT
    ATCGGAAAAGTTCAATCTGTTGGTGATAAAGGGTGGAACTTGGCTGGGATTGCATCTGAG
    ATTTATCGAGGCAATTTAAATAAAGCTTATGCAGATGTTGAACTTAAAGGTTTAAATACT
    CGTGCAGGTGCTATAGTAGGACATTTAGCTCATGATTTTAATCCAAGAGATGTTGGTGCT
    TGGTCTAATGTAAGAAATTCTGTAGCCAAAGGTACTATTGATGTTGCTAGAGGTGATGTA
    GGCGGTTTAATTGGTAAAGACTGGCCTTGGGGAACCATTGATAACAATATCAGCTTAGTG
    AATGTTAAGCGTGGTGATATTTTCTTTGGTTCTAATGATATGGATGATGAATTTGTAAGC
    AATTGGATTAAACCTAATAATGCAACAGTTATCGGTGTGAGTTCTGGAGATAAAACCCAT
    AAACAATCGTCTAAAATTAAAGAAATTTCACAGGTAGAAGCTGATAAGAAAATTAAGGAT
    ATGGGTATCACTGCTGATACATTTGAAGTAACTGAGCATTTGGTTGATAAGTTGAATCGC
    CGTATATTACGTGATGAAGAATACAAAACAATTCAAGATTATAATGCAAATCGTGAGCTT
    GTTTATCGTAATGTTGAAAAACTCCAACCGTTCTACAACAAAGAATGGATTGTGAATCAA
    GGGAACAAACTTACAACTGATAGTCCATTAATGAATAAAGAAGTATTGTCTGTGACTGCA
    ATGAAAGGTAATGACTTTATTACAGACCTTACAGATGCTGACCATATTATGGTTCATTAC
    GCAGATAAGACAAAAGATATCTTTACGATTTCTCCTAAAGACTCTCAAGTTAAGCAAGTA
    AAAGAATACAGTGTCGCAGAGCTTGGTGAAGTCGTCTATACACCAAATATGGTTGTTAAA
    GATCGTGCAGATTTAATTAGTGCTATTGAAGGTATTTTAAGTCCAATTGAATTACAATCT
    GATCCAATTTATCAACGATTAGGAAGAACTGGGGATAACAGAGTTAATGCAATTAAGAAT
    CTTTACTTAGAAGAAAGCTTTAAAGAAGTAAAAGATCATCTTAATACATTTGTGACAAAA
    TTAGTTGAAAATGAAGATCATCAACTGAATACAGATGAAGCTGCAAAACGTGCCTTGATT
    AAGAAAATTGAAGATAACAAAGCTGCTGTCCTGTTAGGATTAACATACCTAAATCGTTAT
    TACGGTGTGAAATTTGATAACTTCAATATTAAAGAATTAATGCTATTCAAACCAGATTTT
    TATGGTAAGAATGTAAGTGTTTTAGATCGATTAATAGAAATTGGTTCAAAAGAGGACTAT
    ATTAAGGGAAGTCGTACACAGGAGTCCTTCCGTGAAGTGGTGGCGAAAAATACTCTTTCT
    GGAAACTTACATGACTTCTTGACCTACAATATGAAACTATTCACAAATGAAACAGACATA
    AATGTTTGGTTTAAGAAAGCTATTGAAAATAATGCTTATGTAGTTGAACAACCGTCAACT
    AACCCTGCATTTGCTAATAAAAAATATCGTCTATATGAAGGACTTAATAACGGACAACAT
    GGACGCATGATTTTATCGCTTCTGAACTTGAAAGATGCACATTTATTTATGATTTCAACA
    TATAATACTATCTCATTTAGTTCGTTTGAAAAGTATGGTAAAGATACAGAAGAAAAACGT
    GAAACGTTCAAGAGTGAAATCAATAAACGTGCTAAAGAGCAAGTTAATTACTTAGATTTT
    TGGTCACGCCTTGCCACTGATAATGTCCGCGATAAACTTCTTAAGAGTCAAAATGTAGTT
    CCAACTCCTGTATGGGATAATCATGGTGCACCCGGTGGTTGGCCTGATAGATTTGGTCAT
    CGAAAAGATAAACCGGATTATGCACCTATTCGTGAATTCTTTGGACGTATTGATAAGTAT
    CATGATTATAAGTATGGGTATGGAGCGTATGCTTACATCTTTGCTGATCCACAGCCTATG
    GATGCTGTATATTTTGTTATGTCGGATTTAATAAGTGAATATGGCACATCTGCCTTTACA
    CATGAGACTACCCACGTAAATGACCGAATGGCATATCTGGGAGGTCATAGACATCGACAG
    GGTACAGATCTTGAAGCATTTGCTCAAGGTATGTTACAAACTCCAGCAGAACATGGACAT
    CAAGGTGAATATGGCGCACTTGGATTAAATATGGCATTTGAACGTTCAAATGATGGAAAC
    CAATGGTATAATCCAGATCCTACCAAACTTCAAACACGTGACCAAATCGACCATTACATG
    AAGAACTACAATGAAGCTATGATGATGCTCGATTATGCAGAAGCTGAGGCTGTTCTTCCT
    AAGGTTAAAGGTGACAATAGTAAGTGGTTCAAGAAAATTGATCGCGAAACACGTCGACCA
    ATGGACAGAAATAAACTAAGTGGTCCTCACCAATGGGACAAAGTGCGTGATTTGATAGAC
    GCTGAGAAAACAACTAACCTTGAAACCATCGATGACTTGGTAAATAATAATTTCATGACC
    ATTCACGGAAATCCAGGTAATAAGGTCTTCCACCCAGAGGACTTTGGTACGGCATATGTC
    AATGTTAATATGATGGCTGGTATTTATGGCGGTAATACGAGTCAAGGTGCCCCTGGTTCT
    CTATCCTTCAAACATAACGCCTTCCGTATGTGGGGTTACTATGGATATGAACATGGGTTC
    ATTGACTATGTTTCAAGCAAACATCAAGGTGCAGCCAATAAAGAAAATAACGGTCTATTA
    GGTGATGATTTCATTATTAAGAAAGTTTCTAATAACCAATTTTCAAACCTTGAAGATTGG
    AAGAAGCAATGGTATGGGGAAGTTCTTACAAAATCTAAAAAAGGTTTTGCACCAATTGAA
    ATTAATGGCACTCAAATTTCAACCTATGAACAGTTGAAGACTATGTTTGATGAGGCTGTT
    ACTAAAGATTTAACGGAACTAAACGATAGTAGTATTAAAGACAAATACCGAAATACAGTT
    GTCTTGAAATCGAAAGTCTTTAAAGCTCTCCTTAAAAACACAGACGGTTTCTCTGGTGAT
    CTATTTACTGCACCACAAGCATAG
    60
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    4140
    4200
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    5220
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    5400
    5460
    5520
    5580
    5640
    5664

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPAP_RS03230 [old locus tag: SPAP_0653 ]
  • symbol: SPAP_RS03230
  • description: G5 domain-containing protein
  • length: 1887
  • theoretical pI: 4.96029
  • theoretical MW: 211796
  • GRAVY: -0.658347

⊟Function[edit | edit source]

  • TIGRFAM:
    Cell structure Cell envelope Other LPXTG cell wall anchor domain (TIGR01167; HMM-score: 26.3)
  • TheSEED: see SPAP_0653
  • PFAM:
    no clan defined Peptidase_M26_C; M26 IgA1-specific Metallo-endopeptidase C-terminal region (PF07580; HMM-score: 774.7)
    and 6 more
    Peptidase_M26_N; M26 IgA1-specific Metallo-endopeptidase N-terminal region (PF05342; HMM-score: 291.1)
    G5 (CL0593) G5; G5 domain (PF07501; HMM-score: 71.1)
    no clan defined Gram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 29.8)
    E-set (CL0159) fn3; Fibronectin type III domain (PF00041; HMM-score: 15.2)
    no clan defined Glug; The GLUG motif (PF07581; HMM-score: 14.1)
    YSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 10.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 10
    • Extracellular Score: 0
    • Internal Helices: 2
  • SignalP: Signal peptide SP(Sec/SPI) length 33 aa
    • SP(Sec/SPI): 0.98483
    • TAT(Tat/SPI): 0.008282
    • LIPO(Sec/SPII): 0.001095
    • Cleavage Site: CS pos: 33-34. VDA-AT. Pr: 0.9782
  • predicted transmembrane helices (TMHMM): 3

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_000473007 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEIISQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGLLTVGSLLLIYKRKKIASVFLVGTMGLVVLPSAGAVDPVATLALASREGVVEMEGYRYVGYLSGDILKTLGLDTVLEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEEAPKEEAPKTEESPKEEPKSEVKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKPEEKVAVKPESQPSDKPAEESKVEQAGEPVAPRKDEQAPVEPENQPEAPEEEKAVEETPKQEESTPDTKAEETVEPKEETKTAKGTQEEDYVGEALVPEQLPEYKVTEGTLVEESTTELDYKTETTEDPTKYTDEETVLRNGEKGSQVTKTTYKTIGGVKTDQVLSTSTEVTKEPVNQQVIRGTKPIEGTTVETREEIIPFETKEQEDDTLKRGTRQVTQEGVNDKKQITETYKTIRGEKTSDAPTITETVIEKPQDKIIKNGTKELEKPTLEWAKTDKDVLKKSATASYTLTKPAGVEIKSIKVALKDNTGTVVKEVTVEENNLQATLDNLKYYQGYTLSTTMVYDRGNGEETEILEDKEVQLDLKKVEIKDIKETRLMKVDENGNETDSSLLETVPENLTSYYLKITTNHNKVTRLAITNIEEVTEEETSLFKVTASAPDLIQRDSNNSLSQTYTYYIEKPKASQNNVYYNFKDLVDAMQKNPNGEFKLGSDLNATNVPTPSKSYVTGKFTGHLTSVDGKHFSIHNTARPLFDRVEGGSIKNINLINVNINMPWANQLASLAQTVKNATVENIKVTGTIVGNNNVAGILDKLDDAGIMTNVAFIGKVQSVGDKGWNLAGIASEIYRGNLNKAYADVELKGLNTRAGAIVGHLAHDFNPRDVGAWSNVRNSVAKGTIDVARGDVGGLIGKDWPWGTIDNNISLVNVKRGDIFFGSNDMDDEFVSNWIKPNNATVIGVSSGDKTHKQSSKIKEISQVEADKKIKDMGITADTFEVTEHLVDKLNRRILRDEEYKTIQDYNANRELVYRNVEKLQPFYNKEWIVNQGNKLTTDSPLMNKEVLSVTAMKGNDFITDLTDADHIMVHYADKTKDIFTISPKDSQVKQVKEYSVAELGEVVYTPNMVVKDRADLISAIEGILSPIELQSDPIYQRLGRTGDNRVNAIKNLYLEESFKEVKDHLNTFVTKLVENEDHQLNTDEAAKRALIKKIEDNKAAVLLGLTYLNRYYGVKFDNFNIKELMLFKPDFYGKNVSVLDRLIEIGSKEDYIKGSRTQESFREVVAKNTLSGNLHDFLTYNMKLFTNETDINVWFKKAIENNAYVVEQPSTNPAFANKKYRLYEGLNNGQHGRMILSLLNLKDAHLFMISTYNTISFSSFEKYGKDTEEKRETFKSEINKRAKEQVNYLDFWSRLATDNVRDKLLKSQNVVPTPVWDNHGAPGGWPDRFGHRKDKPDYAPIREFFGRIDKYHDYKYGYGAYAYIFADPQPMDAVYFVMSDLISEYGTSAFTHETTHVNDRMAYLGGHRHRQGTDLEAFAQGMLQTPAEHGHQGEYGALGLNMAFERSNDGNQWYNPDPTKLQTRDQIDHYMKNYNEAMMMLDYAEAEAVLPKVKGDNSKWFKKIDRETRRPMDRNKLSGPHQWDKVRDLIDAEKTTNLETIDDLVNNNFMTIHGNPGNKVFHPEDFGTAYVNVNMMAGIYGGNTSQGAPGSLSFKHNAFRMWGYYGYEHGFIDYVSSKHQGAANKENNGLLGDDFIIKKVSNNQFSNLEDWKKQWYGEVLTKSKKGFAPIEINGTQISTYEQLKTMFDEAVTKDLTELNDSSIKDKYRNTVVLKSKVFKALLKNTDGFSGDLFTAPQA

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Damiana Chiavolini, Guido Memmi, Tiziana Maggi, Francesco Iannelli, Gianni Pozzi, Marco R Oggioni
The three extra-cellular zinc metalloproteinases of Streptococcus pneumoniae have a different impact on virulence in mice.
BMC Microbiol: 2003, 3;14
[PubMed:12841855] [WorldCat.org] [DOI] (I e)C E Blue, G K Paterson, A R Kerr, M Bergé, J P Claverys, T J Mitchell
ZmpB, a novel virulence factor of Streptococcus pneumoniae that induces tumor necrosis factor alpha production in the respiratory tract.
Infect Immun: 2003, 71(9);4925-35
[PubMed:12933834] [WorldCat.org] [DOI] (P p)

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