⊟Summary[edit | edit source]

PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BM6001

serotype 19F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7465

serotype 1

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 30-JAN-2014

⊟Summary

• organism: Streptococcus pneumoniae G54
locus tag: SPG_1901 [new locus tag: SPG_RS09510 ]
pan locus tag^?: PNEUPAN003620000
symbol: SPG_1901
pan gene symbol^?: ccpA
• synonym:
• product: Catabolite control protein CcpA, RegM

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SPG_1901 [new locus tag: SPG_RS09510 ]
• symbol: SPG_1901
• product: Catabolite control protein CcpA, RegM
• replicon: chromosome
• strand: -
• coordinates: 1812444..1813454
• length: 1011
• essential: unknown

⊟Accession numbers

• Location: CP001015 (1812444..1813454) NCBI
• BioCyc: see SPG_RS09510
• MicrobesOnline: 5757168 MicrobesOnline
• PneumoBrowse for strain D39V: SPV_1797 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  
  ATGAATGCAGATGATACAGTAACCATTTATGATGTCGCTCGTGAAGCAGGTGTTTCCATG
  GCGACGGTCAGCCGTGTGGTCAATGGCAATAAAAATGTAAAAGAGAATACCCGTAAAAAA
  GTGCTAGAGGTAATTGATCGCTTGGATTATCGTCCAAATGCAGTTGCGCGTGGTCTTGCA
  AGTAAAAAGACAACCACNGTCGGTGTCGTGATTCCAAATATTACCAATGGTTATTTTTCG
  AGTTTGGCTAAGGGGATTGATGATATTGCAGAAATGTACAAGTACAATATTGTCCTAGCT
  AATAGCGATGAAGATAACGAGAAAGAAGTTTCTGTTGTCAATACCCTCTTTTCAAAGCAG
  GTAGATGGCATTATCTATATGGGTTATCACTTGACAGATAAAATTCGCTCAGAATTTTCG
  CGTTCACGTACTCCGATTGTTCTCGCAGGAACTGTCGATGTTGAGCACCAGTTGCCAAGT
  GTCAATATTGACTATAAGCAAGCAACAATTGATGCAGTGAGTTACCTTGCTAAAGAAAAT
  GAGCGTATTGCTTTCGTTAGCGGTCCGCTAGTGGATGACATCAATGGTAAGGTTCGTTTA
  GTTGGCTACAAGGAAACCTTGAAAAAAGCAGGAATCGCTTATAGTGAGGGTTTGGTATTT
  GAATCTAAATATAGCTATGATGATGGTTACGCCTTAGCAGAGCGTTTGATTTCATCAAAT
  GCAACTGCAGCAGTTGTGACAGGTGATGAGTTGGCAGCAGGAGTCTTGAACGGTTTGGCT
  GATAAGGGTGTTTCTGTGCCAGAAGATTTTGAAATTATTACTAGTGATGATTCACAAATC
  TCACGCTTTACCCGTCCAAACTTGACAACGATTGCCCAACCTCTTTATGACCTTGGTGCC
  ATTAGTATGCGTATGTTGACCAAGATTATGCATAAGGAAGAGTTGGAAGAACGTGAAGTT
  CTCTTACCTCATGGTTTGACAGAACGTAGCTCAACACGAAAACGTAAATAG
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1011
  

⊟Protein

⊟General

• locus tag: SPG_1901 [new locus tag: SPG_RS09510 ]
• symbol: SPG_1901
• description: Catabolite control protein CcpA, RegM
• length: 336
• theoretical pI: 5.32236
• theoretical MW: 37042.7
• GRAVY: -0.253869

⊟Function

• ⊞⊞TIGRFAM:
  Signal transduction Regulatory functions DNA interactions catabolite control protein A (TIGR01481; HMM-score: 577.2)
  and 8 more
  Signal transduction Regulatory functions DNA interactions D-fructose-responsive transcription factor (TIGR02417; HMM-score: 140.2)
  Signal transduction Regulatory functions DNA interactions trehalose operon repressor (TIGR02405; HMM-score: 93.4)
  rhamnose ABC transporter, rhamnose-binding protein (TIGR02637; HMM-score: 18.1)
  Genetic information processing Mobile and extrachromosomal element functions Other addiction module antidote protein, HigA family (TIGR02607; HMM-score: 14.2)
  Signal transduction Regulatory functions DNA interactions addiction module antidote protein, HigA family (TIGR02607; HMM-score: 14.2)
  Signal transduction Regulatory functions Protein interactions addiction module antidote protein, HigA family (TIGR02607; HMM-score: 14.2)
  Genetic information processing Mobile and extrachromosomal element functions Prophage functions phage-associated protein, BcepMu gp16 family (TIGR04111; HMM-score: 12.8)
  Signal transduction Regulatory functions DNA interactions transcriptional repressor BetI (TIGR03384; HMM-score: 12.2)
• TheSEED  :
  • Catabolite control protein A
  Regulation and Cell signaling Regulation and Cell signaling - no subcategory HPr catabolite repression system  Catabolite control protein A
• ⊞⊞PFAM:
  Periplas_BP (CL0144) Peripla_BP_3; Periplasmic binding protein-like domain (PF13377; HMM-score: 92.1)
  Peripla_BP_1; Periplasmic binding proteins and sugar binding domain of LacI family (PF00532; HMM-score: 78)
  HTH (CL0123) LacI; Bacterial regulatory proteins, lacI family (PF00356; HMM-score: 73.7)
  and 7 more
  Periplas_BP (CL0144) Peripla_BP_4; Periplasmic binding protein domain (PF13407; HMM-score: 58.3)
  HTH (CL0123) HTH_26; Cro/C1-type HTH DNA-binding domain (PF13443; HMM-score: 16.3)
  HTH_3; Helix-turn-helix (PF01381; HMM-score: 16)
  HTH_31; Helix-turn-helix domain (PF13560; HMM-score: 14.1)
  no clan defined DUF5886; Family of unknown function (DUF5886) (PF19233; HMM-score: 13.9)
  HTH (CL0123) Trp_repressor; Trp repressor protein (PF01371; HMM-score: 12.7)
  no clan defined DUF6148; Family of unknown function (DUF6148) (PF19645; HMM-score: 12.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors: Fructose-1,6-bisphosphate; HPr, phosphocarrier protein
• ⊞⊞genes regulated by CcpA*, TF important in Global catabolite repressionRegPrecise

  strH*

  agaR*

  bgaC* > gadV* > gadW* > gadE* > gadF*

  ABC-MSP_1* > ABC-MSP_2* > ABC-SBP_1*

  SPG_0222 > SPG_0223

  pulA

  manN* < manM* < manL*

  hyl

  gno*

  PTS-EII_3* > ugl* > PTS-EII_4* > PTS-EII_5* > PTS-EII_6* > yajC-1* > SPG_0297

  mtlA* > mtlR* > mtlA2* > mtlD*

  tig

  shetA*

  SPG_0418

  pfl*

  xylR*

  PTS-EII_7* > SPG_0430 > SPG_0431

  endoD*

  licT > bglF* > bglA-2*

  fba

  ABC-NBD_2* > SPG_0580 > SPG_0581

  gki

  lctO*

  guaB_1*

  SPG_0689

  ripA > deoB* > SPG_0751 > pnp

  coaA

  bbmA_3*

  yjbK*

  yjbM* > ppnK* > rluD3*

  glgB > glgC > glgD > glgA

  SPG_1045

  eno

  lacG < lacE-2* < lacF-2* < lacT*

  PTS-EII_13*

  nirC*

  ldh

  bglK_1* < iolX_1* < nanC < SPG_1220 < SPG_1221 < nanA_1* < nanE* < rpiR*

  queA

  Pn-aqpC*

  pgl*

  msmK*

  entB* < codY

  merA*

  phnA*

  satC* < satB* < satA*

  nanP* < nanE

  qsrB* < qsrA*

  scrK* < scrA*

  galT-2* < galK*

  galR*

  treC < treP*

  treR*

  msmG* < msmF* < msmE* < agaN*

  msmR

  pflA

  ccpA*

  adhE*

  ackA

  glgP < malQ

  malX > malC > malD > malA

  arcD* > pepV*

  adh2*

  fucI* < fucA* < SPG_2100 < SPG_2101 < galA* < SPG_2103 < SPG_2104 < sugB* < araP* < msmE_2* < fucK*

  fucR*

  Pn-aqpB* < glpO* < glpK

  SPG_2128

  yfiA

  transcription units transferred from TIGR4 data RegPrecise

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• ⊞⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9689
  • Cytoplasmic Membrane Score: 0.0102
  • Cell wall & surface Score: 0.0002
  • Extracellular Score: 0.0207
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.012656
  • TAT(Tat/SPI): 0.002715
  • LIPO(Sec/SPII): 0.003638
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: ACF54874 NCBI
• UniProt:

⊟Protein sequence

• MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEVIDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANSDEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPSVNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGIAYSEGLVFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSDDSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRKRK

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• ⊞⊞regulator: CcpA* regulon

  CcpA*

  (TF)

  important in Global catabolite repression; RegPrecise

⊟Expression data

• PneumoExpress for strain D39V: SPV_1797 PneumoExpress

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Ramkumar Iyer, Nitin S Baliga, Andrew Camilli
Catabolite control protein A (CcpA) contributes to virulence and regulation of sugar metabolism in Streptococcus pneumoniae.
J Bacteriol: 2005, 187(24);8340-9
[PubMed:16321938] [WorldCat.org] [DOI] (P p)Greer E Kaufman, Janet Yother
CcpA-dependent and -independent control of beta-galactosidase expression in Streptococcus pneumoniae occurs via regulation of an upstream phosphotransferase system-encoding operon.
J Bacteriol: 2007, 189(14);5183-92
[PubMed:17496092] [WorldCat.org] [DOI] (P p)